EM structure of the DNA wrapping in bacterial open transcription initiation complex
Resolution: 17.0 Å
EM Method: Single-particle
Fitted PDBs: 6n4c
Q-score: 0.019
Sosa R, Florez-Ariza A, Diaz-Celis C, Onoa B, Cassago A, de Oliveira PSL, Portugal RV, Guerra DG, Bustamante C
bioRxiv (2020) [ DOI: doi:10.1101/2020.05.13.070375 ]
- Rna polymerase sigma factor rpod (64 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna (94-mer) (28 kDa, DNA from Enterobacteria phage lambda)
- Dna (94-mer) (29 kDa, DNA from Enterobacteria phage lambda)
- Dna-directed rna polymerase subunit alpha (34 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (150 kDa, Protein from Escherichia coli K-12)
- E. coli rnap-dna wrapped open complex (500 kDa, Complex from Escherichia coli BL21)
Kaiser CJO, Peters C, Schmid PWN, Stavropoulou M, Zou J, Dahiya V, Mymrikov EV, Rockel B, Asami S, Haslbeck M, Rappsilber J, Reif B, Zacharias M, Buchner J, Weinkauf S
Nat Struct Mol Biol (2019) 26 pp. 1141-1150 [ DOI: doi:10.1038/s41594-019-0332-9 Pubmed: 31792453 ]
- Alphaa-crystallin (Protein from Homo sapiens)
- Reduced recombinant human alphaa-crystallin (319 kDa, Complex from Homo sapiens)
Kaiser CJO, Peters C, Schmid PWN, Stavropoulou M, Zou J, Dahiya V, Mymrikov EV, Rockel B, Asami S, Haslbeck M, Rappsilber J, Reif B, Zacharias M, Buchner J, Weinkauf S
Nat Struct Mol Biol (2019) 26 pp. 1141-1150 [ DOI: doi:10.1038/s41594-019-0332-9 Pubmed: 31792453 ]
- Alphaa-crystallin (Protein from Homo sapiens)
- Reduced recombinant human alphaa-crystallin (239 kDa, Complex from Homo sapiens)
Kaiser CJO, Peters C, Schmid PWN, Stavropoulou M, Zou J, Dahiya V, Mymrikov EV, Rockel B, Asami S, Haslbeck M, Rappsilber J, Reif B, Zacharias M, Buchner J, Weinkauf S
Nat Struct Mol Biol (2019) 26 pp. 1141-1150 [ DOI: doi:10.1038/s41594-019-0332-9 Pubmed: 31792453 ]
- Reduced recombinant human alphaa-crystallin (398 kDa, Complex from Homo sapiens)
- Alphaa-crystallin (Protein from Homo sapiens)
Negative stain structure of Vps15/Vps34 complex
Resolution: 28.0 Å
EM Method: Single-particle
Fitted PDBs: 5kc2
Q-score: 0.043
Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O, Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi M, Ktistakis NT, Sachse C, Williams RL
Autophagy (2016) 12 pp. 2129-2144 [ Pubmed: 27630019 DOI: doi:10.1080/15548627.2016.1226736 ]
- Serine/threonine-protein kinase vps15 (166 kDa, Protein from Saccharomyces cerevisiae)
- Phosphatidylinositol 3-kinase vps34 (101 kDa, Protein from Saccharomyces cerevisiae)
- Vps15/34 (Complex from Saccharomyces cerevisiae)
Maltose binding protein genetically fused to dodecameric glutamine synthetase
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 5ldf
Q-score: 0.239
Coscia F, Estrozi LF, Hans F, Malet H, Noirclerc-Savoye M, Schoehn G, Petosa C
Sci Rep (2016) 6 pp. 30909-30909 [ Pubmed: 27485862 DOI: doi:10.1038/srep30909 ]
- Maltose-binding periplasmic protein (40 kDa, Protein from Escherichia coli O157:H7)
- Maltose-binding protein genetically fused to glutamine synthetase (1 MDa, Complex from Escherichia coli)
- Glutamine synthetase (51 kDa, Protein from Salmonella typhi)
Cryo-EM of full-length p97-FAF1 protein complex
Ewens CA, Panico S, Kloppsteck P, McKeown C, Ebong IO, Robinson C, Zhang X, Freemont PS
J Biol Chem (2014) 289 pp. 12077-12084 [ DOI: doi:10.1074/jbc.M114.559591 Pubmed: 24619421 ]
- P97 (550 kDa, Protein from Mus musculus)
- Fas-associated factor 1 (230 kDa, Protein from Mus musculus)
- Full-length p97-faf1 (780 kDa, Sample)
Inter-ring rotations of AAA ATPase p97
Yeung HO, Forster A, Bebeacua C, Niwa H, Ewens C, McKeown C, Zhang X, Freemont PS
Open Biol (2014) 4 pp. 130142-130142 [ Pubmed: 24598262 DOI: doi:10.1098/rsob.130142 ]
- Transitional endoplasmic reticulum atpase (90 kDa, Protein from Mus musculus)
- P97 without nucleotide, conformation 1 (550 kDa, Sample)
Inter-ring rotations of AAA ATPase p97
Yeung HO, Forster A, Bebeacua C, Niwa H, Ewens C, McKeown C, Zhang X, Freemont PS
Open Biol (2014) 4 pp. 130142-130142 [ Pubmed: 24598262 DOI: doi:10.1098/rsob.130142 ]
- P97 in presence of adp, conformation 1 (550 kDa, Sample)
- Transitional endoplasmic reticulum atpase (90 kDa, Protein from Mus musculus)
Inter-ring rotations of AAA ATPase p97
Yeung HO, Forster A, Bebeacua C, Niwa H, Ewens C, McKeown C, Zhang X, Freemont PS
Open Biol (2014) 4 pp. 130142-130142 [ Pubmed: 24598262 DOI: doi:10.1098/rsob.130142 ]
- Transitional endoplasmic reticulum atpase (90 kDa, Protein from Mus musculus)
- P97 in presence of atp-gammas, conformation 1 (550 kDa, Sample)