CryoEM Density of Acetylated Microtubules
Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 6o2q
Q-score: 0.428
Eshun-Wilson L, Zhang R, Portran D, Nachury MV, Toso DB, Lohr T, Vendruscolo M, Bonomi M, Fraser JS, Nogales E
PNAS (2019) 116 pp. 10366-10371 [ Pubmed: 31072936 DOI: doi:10.1073/pnas.1900441116 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Acetylated microtubule (Tissue from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
Resolution: 3.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 6o2r
Q-score: 0.461
Eshun-Wilson L, Zhang R, Portran D, Nachury MV, Toso DB, Lohr T, Vendruscolo M, Bonomi M, Fraser JS, Nogales E
PNAS (2019) 116 pp. 10366-10371 [ Pubmed: 31072936 DOI: doi:10.1073/pnas.1900441116 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Deacetylated microtubule (Tissue from Sus scrofa)
13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules decorated with kinesin-1 motor domain
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 6e7b
Q-score: 0.43
Dev Cell (2018) 47 pp. 175- [ DOI: doi:10.1016/j.devcel.2018.08.014 Pubmed: 30245156 ]
- 13-pf 3-start gmpcpp-human alpha1b/beta3 microtubule decorated with kinesin-1 motor domain (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta-3 chain (47 kDa, Protein from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
14-pf 3-start GMPCPP-human alpha1B/beta2B microtubules decorated with kinesin-1 motor domain
Resolution: 3.65 Å
EM Method: Helical reconstruction
Fitted PDBs: 6e7c
Q-score: 0.411
Dev Cell (2018) 47 pp. 175- [ DOI: doi:10.1016/j.devcel.2018.08.014 Pubmed: 30245156 ]
- Tubulin beta-2b chain (47 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 14-pf 3-start gmpcpp-human alpha1b/beta2b microtubule decorated with kinesin-1 motor domain (Complex from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Undecorated GMPCPP microtubule
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6dpu
Q-score: 0.494
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ Pubmed: 29915050 DOI: doi:10.1073/pnas.1802637115 ]
- Magnesium ion (24 Da, Ligand)
- Microtubule stabilized by gmpcpp (Complex from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Resolution: 3.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 6dpv
Q-score: 0.486
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ Pubmed: 29915050 DOI: doi:10.1073/pnas.1802637115 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dynamic microtubule in gdp state (Complex from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
Undecorated GTPgammaS microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 6dpw
Q-score: 0.456
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ Pubmed: 29915050 DOI: doi:10.1073/pnas.1802637115 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Gtpgammas-microtubule polymerized from gmpcpp-microtubule seeds (Complex from Sus scrofa)
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Mu Opioid Receptor-Gi Protein Complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6dde
Q-score: 0.407
Koehl A, Hu H, Maeda S, Zhang Y, Qu Q, Paggi JM, Latorraca NR, Hilger D, Dawson R, Matile H, Schertler GFX, Granier S, Weis WI, Dror RO, Manglik A, Skiniotis G, Kobilka BK
Nature (2018) 558 pp. 547-552 [ DOI: doi:10.1038/s41586-018-0219-7 Pubmed: 29899455 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Damgo (513 Da, Protein from Homo sapiens)
- Ternary complex of damgo-activated mu-type opioid receptor with heterotrimeric gi, further stabilized by addition of scfv-16 (130 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
Mu Opioid Receptor-Gi Protein Complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6ddf
Q-score: 0.416
Koehl A, Hu H, Maeda S, Zhang Y, Qu Q, Paggi JM, Latorraca NR, Hilger D, Dawson R, Matile H, Schertler GFX, Granier S, Weis WI, Dror RO, Manglik A, Skiniotis G, Kobilka BK
Nature (2018) 558 pp. 547-552 [ DOI: doi:10.1038/s41586-018-0219-7 Pubmed: 29899455 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Ternary complex of damgo-activated mu-type opioid receptor with heterotrimeric gi (120 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Damgo (513 Da, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
Atomic resolution cryo-EM structure of beta-galactosidase
Resolution: 1.9 Å
EM Method: Single-particle
Fitted PDBs: 6cvm
Q-score: 0.7
Bartesaghi A, Aguerrebere C, Falconieri V, Banerjee S, Earl LA, Zhu X, Grigorieff N, Milne JLS, Sapiro G, Wu X, Subramaniam S
Structure (2018) 26 pp. 848- [ Pubmed: 29754826 DOI: doi:10.1016/j.str.2018.04.004 ]
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- 2-phenylethyl 1-thio-beta-d-galactopyranoside (300 Da, Ligand)
- Escherichia coli beta-galactosidase bound to phenylethyl beta-d-thiogalactopyranoside (petg) (465 kDa, Complex from Escherichia coli K-12)
- Sodium ion (22 Da, Ligand)
- Beta-galactosidase (116 kDa, Protein from Escherichia coli (strain K12))