Yeast tubulin polymerized with GTP in vitro
Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3f
Q-score: 0.366
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dynamic microtubule lattice (Complex from Saccharomyces cerevisiae S288c)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast microtubule stabilized with epothilone
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3h
Q-score: 0.369
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Epothilone a (493 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Microtubule lattice stabilized with epothilone (Complex from Saccharomyces cerevisiae S288c)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast microtubule stabilized with Taxol assembled from mutated tubulin
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3j
Q-score: 0.338
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Yeast microtubule stabilized with taxol assembled from mutated tubulin (Complex from Saccharomyces cerevisiae S288c)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin beta chain (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Near-atomic cryo-EM structure of PRC1 bound to the microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5kmg
Q-score: 0.41
Kellogg EH, Howes S, Ti SC, Ramirez-Aportela E, Kapoor TM, Chacon P, Nogales E
PNAS (2016) 113 pp. 9430-9439 [ Pubmed: 27493215 DOI: doi:10.1073/pnas.1609903113 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Protein regulator of cytokinesis 1 (15 kDa, Protein from Homo sapiens)
- Tubulin beta chain (48 kDa, Protein from Sus scrofa)
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (49 kDa, Protein from Sus scrofa)
- Ternary complex of prc1 fragment (prc1-sc), alpha-tubulin, and beta-tubulin. (Complex)
Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syc
Q-score: 0.426
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5sye
Q-score: 0.372
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Peloruside a (548 Da, Ligand)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of taxol- and peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syf
Q-score: 0.39
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of taxol-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Cryo-EM reconstruction of zampanolide-bound microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syg
Q-score: 0.303
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- (2z,4e)-n-[(s)-[(1s,2e,5s,8e,10z,17s)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide (497 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of zampanolide-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Cryo EM density of microtubule assembled from human TUBB3
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 5ij0
Q-score: 0.345
Ti SC, Pamula MC, Howes SC, Duellberg C, Cade NI, Kleiner RE, Forth S, Surrey T, Nogales E, Kapoor TM
Dev Cell (2016) 37 pp. 72-84 [ DOI: doi:10.1016/j.devcel.2016.03.003 Pubmed: 27046833 ]
- Microtubule assembled from recombinant human tubulin decorated with kinesin (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta-3 chain (47 kDa, Protein from Homo sapiens)
Cryo EM density of microtubule assembled from human TUBB3-D417H mutant
Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 5ij9
Q-score: 0.401
Ti SC, Pamula MC, Howes SC, Duellberg C, Cade NI, Kleiner RE, Forth S, Surrey T, Nogales E, Kapoor TM
Dev Cell (2016) 37 pp. 72-84 [ DOI: doi:10.1016/j.devcel.2016.03.003 Pubmed: 27046833 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta-3 chain (47 kDa, Protein from Homo sapiens)
- Tubulin alpha-1b chain (48 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Microtubule assembled from recombinant human tubulin beta-3 d417h mutant decorated with kinesin (Complex from Homo sapiens)