Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of PDE6C in complex with inhibitory cone p gamma in the presence of cGMP
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cxg
Q-score: 0.453
Singh S, Srivastava D, Boyd K, Artemyev NO
PNAS (2025) 122 pp. e2419732121-e2419732121 [ Pubmed: 39739818 DOI: doi:10.1073/pnas.2419732121 ]
- Cone cgmp-specific 3',5'-cyclic phosphodiesterase subunit alpha' (97 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-monophosphate (363 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cyclic guanosine monophosphate (345 Da, Ligand)
- Pde6c cone p gamma complex (218 kDa, Complex from Homo sapiens)
- Cone p gamma (13 kDa, Protein from Homo sapiens)
Structure of PDE6C in complex with the rod inhibitory p gamma subunit in the presence of cGMP
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9cxh
Q-score: 0.518
Singh S, Srivastava D, Boyd K, Artemyev NO
PNAS (2025) 122 pp. e2419732121-e2419732121 [ Pubmed: 39739818 DOI: doi:10.1073/pnas.2419732121 ]
- Cone cgmp-specific 3',5'-cyclic phosphodiesterase subunit alpha' (97 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-monophosphate (363 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cone pde6 ion complex with rod p gamma (218 kDa, Complex from Homo sapiens)
- Cyclic guanosine monophosphate (345 Da, Ligand)
- Retinal rod rhodopsin-sensitive cgmp 3',5'-cyclic phosphodiesterase subunit gamma (11 kDa, Protein from Bos taurus)
Structure of PDE6C in complex with the rod inhibitory p gamma subunit in the absence of added cGMP
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cxi
Q-score: 0.523
Singh S, Srivastava D, Boyd K, Artemyev NO
PNAS (2025) 122 pp. e2419732121-e2419732121 [ Pubmed: 39739818 DOI: doi:10.1073/pnas.2419732121 ]
- Magnesium ion (24 Da, Ligand)
- Cone cgmp-specific 3',5'-cyclic phosphodiesterase subunit alpha' (97 kDa, Protein from Homo sapiens)
- Cone pde6 (218 kDa, Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Cyclic guanosine monophosphate (345 Da, Ligand)
- Rod pg (11 kDa, Protein from Mus musculus)
Structure of PDE6C in complex with the rod inhibitory p gamma subunit with disordered GafA region
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9cxj
Q-score: 0.445
Singh S, Srivastava D, Boyd K, Artemyev NO
PNAS (2025) 122 pp. e2419732121-e2419732121 [ Pubmed: 39739818 DOI: doi:10.1073/pnas.2419732121 ]
- Cone cgmp-specific 3',5'-cyclic phosphodiesterase subunit alpha' (97 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cone pde6c in complex with rod p gamma (218 kDa, Complex from Homo sapiens)
- Retinal rod rhodopsin-sensitive cgmp 3',5'-cyclic phosphodiesterase subunit gamma (11 kDa, Protein from Bos taurus)
Cryo EM map of full length PLC gamma 2
Resolution: 3.72 Å
EM Method: Single-particle
Fitted PDBs: 8jqg
Q-score: 0.384
Shin YC, Plummer-Medeiros AM, Mungenast A, Choi HW, TenDyke K, Zhu X, Shepard J, Sanders K, Zhuang N, Hu L, Qian D, Song K, Xu C, Wang J, Poda SB, Liao M, Chen Y
Sci Adv (2024) 10 pp. eadn6037-eadn6037 [ DOI: doi:10.1126/sciadv.adn6037 Pubmed: 39612343 ]
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (148 kDa, Protein from Homo sapiens)
- Plcg2 and fgfr1 (147 kDa, Complex from Homo sapiens)