Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Cryo-EM Structure of the 3HO-HCAR3-Gi complex
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8jef
Q-score: 0.517
Ye F, Pan X, Zhang Z, Xiang X, Li X, Zhang B, Ning P, Liu A, Wang Q, Gong K, Li J, Zhu L, Qian C, Chen G, Du Y
Cell Rep (2024) 43 pp. 114895-114895 [ DOI: doi:10.1016/j.celrep.2024.114895 Pubmed: 39427321 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (3s)-3-hydroxyoctanoic acid (160 Da, Ligand)
- Cryo-em structure of the apo hcar3-gi complex (Complex from Homo sapiens)
- Hydroxycarboxylic acid receptor 3 (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 8xdl
Q-score: 0.628
Huang M, Yan P, Wang G, Li J, Wang L, Liu T
To Be Published
- Adenosine-5'-diphosphate (427 Da, Ligand)
- F-actin-end (Complex from Oryctolagus cuniculus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8xdm
Q-score: 0.626
Huang M, Yan P, Wang G, Li J, Wang L, Liu T
To Be Published
- Adenosine-5'-diphosphate (427 Da, Ligand)
- F-actin-mad (Complex from Oryctolagus cuniculus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of Ca2+-bound TMEM16A in complex with Tamsulosin
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8xlr
Q-score: 0.527
Li S, Sun W, Li S, Zhu L, Guo S, He J, Li Y, Tian C, Zhao Z, Yu T, Li J, Zhang Y, Hai Y, Wang J, Zheng Y, Wang R, Hu X, Ling S, Li H, Li Y
PNAS (2025) 122 pp. e2407493121-e2407493121 [ Pubmed: 39739807 DOI: doi:10.1073/pnas.2407493121 ]
- Tamsulosin (408 Da, Ligand)
- Hexane (86 Da, Ligand)
- Tetradecane (198 Da, Ligand)
- Anoctamin-1 (111 kDa, Protein from Mus musculus)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Structure of tamsulosin- and calcium-bound mtmem16a chloride channel (Complex from Mus musculus)
Cryo-EM structure of Apoferritin collected by EPU on Glacios at 2.5 Angstrom resolution
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8tu7
Q-score: 0.678
Jia L, Ruben EA, Olsen SK, Wasmuth EV
To Be Published
- Apoferritin (Cell from Mus musculus)
- Ferritin heavy chain (21 kDa, Protein from Mus musculus)
- Fe (iii) ion (55 Da, Ligand)
- Zinc ion (65 Da, Ligand)