Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Cryo-EM structure of human 26S proteasomal RP subcomplex (Ea state) without any bound substrate.
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8jri
Q-score: 0.443
Draczkowski P, Hsu STD, Wang YS
To Be Published
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Human 26s proteasome (Complex from Homo sapiens)
- Aaa+-atpase of 19s regulatory particle (rp) of human 26s proteasome. (Complex from Homo sapiens)
- 26s protease regulatory subunit 6b (47 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 10b (44 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 19s regulatory particle (rp) of human 26s proteasome. (Complex from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 12 (52 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 11 (47 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 7 (37 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 8 (39 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 8 (45 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 13 (42 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 6a (49 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 7 (48 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 6 (45 kDa, Protein from Homo sapiens)
- 26s proteasome complex subunit dss1 (8 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome non-atpase regulatory subunit 4 (40 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 4 (49 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 1 (105 kDa, Protein from Homo sapiens)
- Alpha subunits belonging to the 20s core particle (cp) of human 26s proteasome. (Complex from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 14 (34 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 3 (61 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 2 (100 kDa, Protein from Homo sapiens)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8jrt
Q-score: 0.423
Draczkowski P, Hsu STD, Wang YS
To Be Published
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Human 26s proteasome (Complex from Homo sapiens)
- 26s protease regulatory subunit 6b (47 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 10b (44 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 26s proteasome non-atpase regulatory subunit 12 (52 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 11 (47 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 8 (39 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 7 (37 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 8 (45 kDa, Protein from Homo sapiens)
- 19s regulatory particle (rp) of human 26s proteasome (Complex from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 13 (42 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 6a (49 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 7 (48 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- Aaa+-atpase of 19s regulatory particle (rp) of human 26s proteasome (Complex from Homo sapiens)
- K11/k48-branched ubiquitin (ub) chain composed of three ub (Complex from Homo sapiens)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 6 (45 kDa, Protein from Homo sapiens)
- 26s proteasome complex subunit dss1 (8 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome non-atpase regulatory subunit 4 (40 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- Alpha subunits belonging to the 20s core particle (cp) of human 26s proteasome (Complex from Homo sapiens)
- 26s protease regulatory subunit 4 (49 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 1 (105 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 14 (34 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 3 (61 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 2 (100 kDa, Protein from Homo sapiens)
Cryo-EM structure of human CD163 SRCR2-4 in complex with haptoglobin-hemoglobin
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 9fhb
Q-score: 0.405
Etzerodt A, Mikkelsen JH, Torvund-Jensen M, Hennig D, Boesen T, Graversen JH, Moestrup SK, Kollman JM, Andersen CBF
Nat Commun (2024) 15 pp. 10871-10871 [ DOI: doi:10.1038/s41467-024-55171-4 Pubmed: 39738064 ]
- Cd163 (Complex from Homo sapiens)
- Scavenger receptor cysteine-rich type 1 protein m130 (125 kDa, Protein from Homo sapiens)
- Cd163 in complex with haptoglobin-hemoglobin (Complex)
- Haptoglobin (45 kDa, Protein from Homo sapiens)
- Hemoglobin subunit alpha (15 kDa, Protein from Homo sapiens)
- Hemoglobin subunit beta (16 kDa, Protein from Homo sapiens)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Haptoglobin-hemoglobin complex (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of human CD163 SRCR1-9 in complex with haptoglobin-hemoglobin
Resolution: 4.46 Å
EM Method: Single-particle
Fitted PDBs: 9fmu
Q-score: 0.233
Etzerodt A, Mikkelsen JH, Torvund-Jensen M, Hennig D, Boesen T, Graversen JH, Moestrup SK, Kollman JM, Andersen CBF
Nat Commun (2024) 15 pp. 10871-10871 [ DOI: doi:10.1038/s41467-024-55171-4 Pubmed: 39738064 ]
- Isoform 2 of haptoglobin (38 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hemopressin (15 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Scavenger receptor cysteine-rich type 1 protein m130 (112 kDa, Protein from Homo sapiens)
- Spinorphin (16 kDa, Protein from Homo sapiens)
- Cd163 in complex with haptoglobin-hemoglobin (Complex from Homo sapiens)
Resolution: 1.78 Å
EM Method: Single-particle
Fitted PDBs: 8qyx
Q-score: 0.703
Wiechert F, Unbehaun A, Sprink T, Seibel H, Burger J, Loerke J, Mielke T, Diebolder CA, Schacherl M, Spahn CMT
Nucleic Acids Res (2025) 53 [ DOI: doi:10.1093/nar/gkae1191 Pubmed: 39658079 ]
- Spermine (202 Da, Ligand)
- 1,4-diaminobutane (88 Da, Ligand)
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- Human mature large ribosomal subunit (Complex from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul2 (28 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul30 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- Spermidine (145 Da, Ligand)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)