afTMEM16 in C22 lipid nanodiscs with MSP2N2 scaffold protein in the presnece of Ca2+
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7rwj
Q-score: 0.484
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Aftmem16 scramblase c22 lipid nanodiscs with msp2n2 scaffold in the presence of ca2+ (Complex from Aspergillus fumigatus)
- Plasma membrane channel protein (aqy1), putative (84 kDa, Protein from Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100))
afTMEM16 in C22 lipid nanodiscs with MSP1E3 scaffold protein in the presnece of Ca2+
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7rx2
Q-score: 0.632
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- Water (18 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 scramblase c22 lipid nanodiscs with msp1e3 scaffold in the presence of ca2+ (Complex from Aspergillus fumigatus)
- Calcium ion (40 Da, Ligand)
afTMEM16 in C14 lipid nanodiscs with MSP1E3 scaffold protein in the absence of Ca2+
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7rx3
Q-score: 0.533
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- Aftmem16 scramblase in c14 lipid nanodiscs with msp1e3 scaffold in the absence of ca2+ (Complex from Aspergillus fumigatus)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
afTMEM16 DE/AA mutant in C14 lipid nanodiscs in the presence of Ca2+
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7rxa
Q-score: 0.558
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 de/aa mutant in c14 lipid nanodiscs in the presence of ca2+ (Complex from Aspergillus fumigatus)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
afTMEM16 lipid scramblase in C18 lipid nanodiscs in the absence of Ca2+
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 7rxb
Q-score: 0.595
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 lipid scramblase in c18 lipid nanodiscs in the absence of ca2+ (Complex from Aspergillus fumigatus)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
afTMEM16 in C18 lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+, full dimer
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 7rxg
Q-score: 0.648
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- Water (18 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 scramblase c18 lipid nanodiscs with msp1e3 scaffold in the presence of ca2+, full dimer (Complex from Aspergillus fumigatus)
- Calcium ion (40 Da, Ligand)
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7rxh
Q-score: 0.556
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- Aftmem16 scramblase c18 lipid nanodiscs with msp1e3 scaffold in the presence of ca2+, monomer with additional lipids (Complex from Aspergillus fumigatus)
- Water (18 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Calcium ion (40 Da, Ligand)
nhTMEM16 L302A +Ca2+ in nanodiscs
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6oy3
Q-score: 0.456
Khelashvili G, Falzone ME, Cheng X, Lee BC, Accardi A, Weinstein H
Nat Commun (2019) 10 pp. 4972-4972 [ Pubmed: 31672969 DOI: doi:10.1038/s41467-019-12865-4 ]
CryoEM structure of calcium-bound human TMEM16K / Anoctamin 10 in detergent (2mM Ca2+, closed form)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 6r7x
Q-score: 0.516
Bushell SR, Pike ACW, Falzone ME, Rorsman NJG, Ta CM, Corey RA, Newport TD, Christianson JC, Scofano LF, Shintre CA, Tessitore A, Chu A, Wang Q, Shrestha L, Mukhopadhyay SMM, Love JD, Burgess-Brown NA, Sitsapesan R, Stansfeld PJ, Huiskonen JT, Tammaro P, Accardi A, Carpenter EP
Nat Commun (2019) 10 pp. 3956-3956 [ Pubmed: 31477691 DOI: doi:10.1038/s41467-019-11753-1 ]
- Anoctamin 10 (153 kDa, Cellular component from Homo sapiens)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Undecyl-maltoside (496 Da, Ligand)
- Anoctamin-10 (77 kDa, Protein from Homo sapiens)
CryoEM structure of calcium-bound human TMEM16K / Anoctamin 10 in detergent (low Ca2+, closed form)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6r7y
Q-score: 0.417
Bushell SR, Pike ACW, Falzone ME, Rorsman NJG, Ta CM, Corey RA, Newport TD, Christianson JC, Scofano LF, Shintre CA, Tessitore A, Chu A, Wang Q, Shrestha L, Mukhopadhyay SMM, Love JD, Burgess-Brown NA, Sitsapesan R, Stansfeld PJ, Huiskonen JT, Tammaro P, Accardi A, Carpenter EP
Nat Commun (2019) 10 pp. 3956-3956 [ Pubmed: 31477691 DOI: doi:10.1038/s41467-019-11753-1 ]
- Anoctamin 10 (153 kDa, Cellular component from Homo sapiens)
- Anoctamin-10 (77 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)