Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8uc7
Q-score: 0.553
Kim ED, Wu X, Lee S, Tibbs GR, Cunningham KP, Di Zanni E, Perez ME, Goldstein PA, Accardi A, Larsson HP, Nimigean CM
Nature (2024) 632 pp. 451-459 [ Pubmed: 39085604 DOI: doi:10.1038/s41586-024-07743-z ]
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (74 kDa, Protein from Homo sapiens)
- Hcn1 with propofol (Complex from Homo sapiens)
- 2,6-bis(1-methylethyl)phenol (178 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8uc8
Q-score: 0.539
Kim ED, Wu X, Lee S, Tibbs GR, Cunningham KP, Di Zanni E, Perez ME, Goldstein PA, Accardi A, Larsson HP, Nimigean CM
Nature (2024) 632 pp. 451-459 [ Pubmed: 39085604 DOI: doi:10.1038/s41586-024-07743-z ]
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 9bc6
Q-score: 0.625
Kim ED, Wu X, Lee S, Tibbs GR, Cunningham KP, Di Zanni E, Perez ME, Goldstein PA, Accardi A, Larsson HP, Nimigean CM
Nature (2024) 632 pp. 451-459 [ Pubmed: 39085604 DOI: doi:10.1038/s41586-024-07743-z ]
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (74 kDa, Protein from Homo sapiens)
- 2,6-bis(1-methylethyl)phenol (178 Da, Ligand)
- Hcn1 (Complex from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9bc7
Q-score: 0.496
Kim ED, Wu X, Lee S, Tibbs GR, Cunningham KP, Di Zanni E, Perez ME, Goldstein PA, Accardi A, Larsson HP, Nimigean CM
Nature (2024) 632 pp. 451-459 [ Pubmed: 39085604 DOI: doi:10.1038/s41586-024-07743-z ]
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (74 kDa, Protein from Homo sapiens)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Hcn1 (Complex from Homo sapiens)
CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Swap
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8ga1
Q-score: 0.626
Fortea E, Lee S, Chadda R, Argyros Y, Sandal P, Mahoney-Kruszka R, Ciftci HD, Falzone ME, Huysmans G, Robertson JL, Boudker O, Accardi A
Nat Struct Mol Biol (2024) 31 pp. 644-656 [ Pubmed: 38279055 DOI: doi:10.1038/s41594-023-01210-5 ]
- H(+)/cl(-) exchange transporter clca (49 kDa, Protein from Escherichia coli)
- Chloride ion (35 Da, Ligand)
- Water (18 Da, Ligand)
- Structure of ecclc-r230c/l249c/c85a mutant at ph 4.5 in 100mm cl (Cell from Escherichia coli)
CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Turn
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8ga3
Q-score: 0.529
Fortea E, Lee S, Chadda R, Argyros Y, Sandal P, Mahoney-Kruszka R, Ciftci HD, Falzone ME, Huysmans G, Robertson JL, Boudker O, Accardi A
Nat Struct Mol Biol (2024) 31 pp. 644-656 [ Pubmed: 38279055 DOI: doi:10.1038/s41594-023-01210-5 ]
CLC-ec1 L25C/A450C/C85A at pH 4.5 100mM Cl Intermediate
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8ga5
Q-score: 0.626
Fortea E, Lee S, Chadda R, Argyros Y, Sandal P, Mahoney-Kruszka R, Ciftci HD, Falzone ME, Huysmans G, Robertson JL, Boudker O, Accardi A
Nat Struct Mol Biol (2024) 31 pp. 644-656 [ Pubmed: 38279055 DOI: doi:10.1038/s41594-023-01210-5 ]
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- H(+)/cl(-) exchange transporter clca (49 kDa, Protein from Escherichia coli)
- Structure of ecclc-l25c/a450c/c85a mutant at ph 4.5 in 100mm cl (Cell from Escherichia coli)
CLC-ec1 L25C/A450C/C85A at pH 4.5 100mM Cl Twist
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8gah
Q-score: 0.571
Fortea E, Lee S, Chadda R, Argyros Y, Sandal P, Mahoney-Kruszka R, Ciftci HD, Falzone ME, Huysmans G, Robertson JL, Boudker O, Accardi A
Nat Struct Mol Biol (2024) 31 pp. 644-656 [ Pubmed: 38279055 DOI: doi:10.1038/s41594-023-01210-5 ]
afTMEM16 in C22 lipid nanodiscs with MSP2N2 scaffold protein in the presnece of Ca2+
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7rwj
Q-score: 0.484
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Aftmem16 scramblase c22 lipid nanodiscs with msp2n2 scaffold in the presence of ca2+ (Complex from Aspergillus fumigatus)
- Plasma membrane channel protein (aqy1), putative (84 kDa, Protein from Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100))
afTMEM16 in C22 lipid nanodiscs with MSP1E3 scaffold protein in the presnece of Ca2+
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7rx2
Q-score: 0.632
Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A
Nat Commun (2022) 13 pp. 2604-2604 [ DOI: doi:10.1038/s41467-022-30300-z Pubmed: 35562175 ]
- Water (18 Da, Ligand)
- Aftmem16 lipid scramblase (84 kDa, Protein from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163))
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Aftmem16 scramblase c22 lipid nanodiscs with msp1e3 scaffold in the presence of ca2+ (Complex from Aspergillus fumigatus)
- Calcium ion (40 Da, Ligand)