Structure of the prefusion HKU5-19s Spike trimer (conformation 1)
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 9ea0
Q-score: 0.702
Park YJ, Liu C, Lee J, Brown JT, Ma CB, Liu P, Gen R, Xiong Q, Zepeda SK, Stewart C, Addetia A, Craig CJ, Tortorici MA, Alshukairi AN, Starr TN, Yan H, Veesler D
Cell (2025) [ Pubmed: 39922192 DOI: doi:10.1016/j.cell.2024.12.032 ]
- Water (18 Da, Ligand)
- Prefusion hku5-19s s trimer (Complex from Homo sapiens)
- Folic acid (441 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Spike glycoprotein (150 kDa, Protein from Pipistrellus bat coronavirus HKU5)
- Linoleic acid (280 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the prefusion HKU5-19s Spike trimer (conformation 2)
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 9eh8
Q-score: 0.741
Park YJ, Liu C, Lee J, Brown JT, Ma CB, Liu P, Gen R, Xiong Q, Zepeda SK, Stewart C, Addetia A, Craig CJ, Tortorici MA, Alshukairi AN, Starr TN, Yan H, Veesler D
Cell (2025) [ Pubmed: 39922192 DOI: doi:10.1016/j.cell.2024.12.032 ]
- Water (18 Da, Ligand)
- Prefusion hku5-19s s trimer (Complex from Homo sapiens)
- Folic acid (441 Da, Ligand)
- Spike glycoprotein (150 kDa, Protein from Pipistrellus bat coronavirus HKU5)
- Linoleic acid (280 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the HKU5 RBD bound to the P. abramus ACE2 receptor
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9d32
Q-score: 0.564
Park YJ, Liu C, Lee J, Brown JT, Ma CB, Liu P, Gen R, Xiong Q, Zepeda SK, Stewart C, Addetia A, Craig CJ, Tortorici MA, Alshukairi AN, Starr TN, Yan H, Veesler D
Cell (2025) [ Pubmed: 39922192 DOI: doi:10.1016/j.cell.2024.12.032 ]
- Spike glycoprotein (29 kDa, Protein from Pipistrellus bat coronavirus HKU5)
- Angiotensin-converting enzyme (89 kDa, Protein from Pipistrellus abramus)
- Zinc ion (65 Da, Ligand)
- Hku5 rbd bound to the p. abramus ace2 receptor (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the HKU5-19s RBD bound to the Bos taurus ACE2 receptor
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 9e0i
Q-score: 0.676
Park YJ, Liu C, Lee J, Brown JT, Ma CB, Liu P, Gen R, Xiong Q, Zepeda SK, Stewart C, Addetia A, Craig CJ, Tortorici MA, Alshukairi AN, Starr TN, Yan H, Veesler D
Cell (2025) [ Pubmed: 39922192 DOI: doi:10.1016/j.cell.2024.12.032 ]
- Spike glycoprotein (29 kDa, Protein from Pipistrellus bat coronavirus HKU5)
- Water (18 Da, Ligand)
- Hku5-19s rbd bound to the bos taurus ace2 receptor (Complex from Homo sapiens)
- Angiotensin-converting enzyme 2 (89 kDa, Protein from Bos taurus)
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8fxc
Q-score: 0.507
Addetia A, Piccoli L, Case JB, Park YJ, Beltramello M, Guarino B, Dang H, de Melo GD, Pinto D, Sprouse K, Scheaffer SM, Bassi J, Silacci-Fregni C, Muoio F, Dini M, Vincenzetti L, Acosta R, Johnson D, Subramanian S, Saliba C, Giurdanella M, Lombardo G, Leoni G, Culap K, McAlister C, Rajesh A, Dellota Jr E, Zhou J, Farhat N, Bohan D, Noack J, Chen A, Lempp FA, Quispe J, Kergoat L, Larrous F, Cameroni E, Whitener B, Giannini O, Cippa P, Ceschi A, Ferrari P, Franzetti-Pellanda A, Biggiogero M, Garzoni C, Zappi S, Bernasconi L, Kim MJ, Rosen LE, Schnell G, Czudnochowski N, Benigni F, Franko N, Logue JK, Yoshiyama C, Stewart C, Chu H, Bourhy H, Schmid MA, Purcell LA, Snell G, Lanzavecchia A, Diamond MS, Corti D, Veesler D
Nature (2023) 621 pp. 592-601 [ Pubmed: 37648855 DOI: doi:10.1038/s41586-023-06487-6 ]
- Spike protein s1 (28 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 bq.1.1 spike rbd bound to the human ace2 ectodomain and the s309 neutralizing antibody fab fragment (Complex from Severe acute respiratory syndrome coronavirus 2)
- S309 light chain (23 kDa, Protein from Homo sapiens)
- Sars-cov-2 bq.1.1 spike rbd bound to the human ace2 ectodomain and the s309 neutralizing antibody fab fragment (Complex from Severe acute respiratory syndrome coronavirus 2)
- S309 heavy chain (50 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Processed angiotensin-converting enzyme 2 (74 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)