Cryo-EM structure of S. aureus BlaR1 with C2 symmetry
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8exp
Q-score: 0.178
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ Pubmed: 36599987 DOI: doi:10.1038/s41586-022-05583-3 ]
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Dimeric complex of s. aureus blar1 (142 kDa, Complex from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of S. aureus BlaR1 with C1 symmetry
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 8exq
Q-score: 0.122
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ Pubmed: 36599987 DOI: doi:10.1038/s41586-022-05583-3 ]
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Dimeric complex of s. aureus blar1 (142 kDa, Complex from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of S. aureus BlaR1 TM and zinc metalloprotease domain
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8exr
Q-score: 0.324
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ Pubmed: 36599987 DOI: doi:10.1038/s41586-022-05583-3 ]
- Phosphate ion (94 Da, Ligand)
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Dimeric complex of s. aureus blar1 (142 kDa, Complex from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
- (2s)-3-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-2-[(6e)-hexadec-6-enoyloxy]propyl (8e)-octadec-8-enoate (746 Da, Ligand)
Cryo-EM structure of S. aureus BlaR1 F284A mutant
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 8exs
Q-score: 0.203
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ Pubmed: 36599987 DOI: doi:10.1038/s41586-022-05583-3 ]
- Dimeric complex of s. aureus blar1 f284a mutant (142 kDa, Complex from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
Cryo-EM structure of S. aureus BlaR1 F284A mutant in complex with ampicillin
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8ext
Q-score: 0.146
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ Pubmed: 36599987 DOI: doi:10.1038/s41586-022-05583-3 ]
- Dimeric complex of s. aureus blar1 f284a mutant in complex with ampicillin (142 kDa, Complex from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
PmtCD peptide toxin ABC exporter in nucleotide free (apo) conformation
Zeytuni N, Dickey SW, Hu J, Chou HT, Worrall LJ, Alexander JAN, Carlson ML, Nosella M, Duong F, Yu Z, Otto M, Strynadka NCJ
Sci Adv (2020) 6 pp. eabb8219- [ DOI: doi:10.1126/sciadv.abb8219 Pubmed: 32998902 ]
- Pmtd (Protein from Staphylococcus aureus)
- Pmtcd (120 kDa, Complex from Staphylococcus aureus)
- Pmtc (Protein from Staphylococcus aureus)
Zeytuni N, Dickey SW, Hu J, Chou HT, Worrall LJ, Alexander JAN, Carlson ML, Nosella M, Duong F, Yu Z, Otto M, Strynadka NCJ
Sci Adv (2020) 6 pp. eabb8219- [ DOI: doi:10.1126/sciadv.abb8219 Pubmed: 32998902 ]
PmtCD ABC exporter without the basket domain at C2 symmetry
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6xjh
Q-score: 0.409
Zeytuni N, Dickey SW, Hu J, Chou HT, Worrall LJ, Alexander JAN, Carlson ML, Nosella M, Duong F, Yu Z, Otto M, Strynadka NCJ
Sci Adv (2020) 6 pp. eabb8219- [ DOI: doi:10.1126/sciadv.abb8219 Pubmed: 32998902 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Abc transporter atp-binding protein (33 kDa, Protein from Staphylococcus aureus )
- Phenol-soluble modulin export abc transporter permease subunit pmtd (30 kDa, Protein from Staphylococcus aureus)
- Pmtcd (120 kDa, Complex from Staphylococcus aureus)
- Magnesium ion (24 Da, Ligand)
PmtCD ABC exporter at C1 symmetry
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6xji
Q-score: 0.341
Zeytuni N, Dickey SW, Hu J, Chou HT, Worrall LJ, Alexander JAN, Carlson ML, Nosella M, Duong F, Yu Z, Otto M, Strynadka NCJ
Sci Adv (2020) 6 pp. eabb8219- [ DOI: doi:10.1126/sciadv.abb8219 Pubmed: 32998902 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Abc transporter atp-binding protein (33 kDa, Protein from Staphylococcus aureus)
- Phenol-soluble modulin export abc transporter permease subunit pmtd (30 kDa, Protein from Staphylococcus aureus)
- Pmtcd (120 kDa, Complex from Staphylococcus aureus)
- Magnesium ion (24 Da, Ligand)