A microtubule RELION-based pipeline for cryo-EM image processing
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J Struct Biol (2019) [ DOI: 10.1016/j.jsb.2019.10.004 Pubmed: 31610239 ]
Image sets:
A microtubule RELION-based pipeline for cryo-EM image processing
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J Struct Biol (2019) [ DOI: 10.1016/j.jsb.2019.10.004 Pubmed: 31610239 ]
Image sets:
Cryo micrographs of microtubules (GDP state) decorated with NDC-NDC chimera of human doublecortin
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J. Struct. Biol. (2019) 209 [ DOI: 10.1016/j.jsb.2019.10.004 Pubmed: 31610239 ]
Image sets:
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6rf8
Q-score: 0.449
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J Struct Biol (2020) 209 pp. 107402-107402 [ DOI: doi:10.1016/j.jsb.2019.10.004 Pubmed: 31610239 ]
- Neuronal migration protein doublecortin (11 kDa, Protein from Homo sapiens)
- Tubulin beta-2b chain (48 kDa, Protein from Bos taurus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Bos taurus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 5-component complex of n-terminal dc domain (ndc) of ndc-ndc chimera (human doublecortin sequence) bound to 13-protofilament gdp-microtubule lattice site composed of 4 tubulin subunits (two alpha-subunits and two beta-subunits) (Complex)
- Tubulin (Complex from Bos taurus)
- Human doublecortin (Complex from Homo sapiens)
HsCKK (human CAMSAP1) decorated 13pf taxol-GDP microtubule
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6qus
Q-score: 0.454
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Calmodulin-regulated spectrin-associated protein 1 (19 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin (Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Calmodulin-regulated spectrin-associated protein 1 (Complex from Homo sapiens)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Hsckk (human camsap1) decorated 13pf taxol-gdp microtubule (Complex)
NgCKK (N.Gruberi CKK) decorated 13pf taxol-GDP microtubule
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6quy
Q-score: 0.434
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Ckk domain protein (20 kDa, Protein from Naegleria gruberi)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- N.gruberi ckk (Complex from Naegleria gruberi)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Tubulin (Complex from Homo sapiens)
- Ngckk (n.gruberi ckk) decorated 13pf taxol-gdp microtubule (Complex)
NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6qve
Q-score: 0.435
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Predicted protein (20 kDa, Protein from Naegleria gruberi)
- Taxol (853 Da, Ligand)
- Beta1-tubulin (49 kDa, Protein from Homo sapiens)
- Ngckk (naegleria gruberi ckk) decorated 14pf taxol-gdp microtubule (Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Naegleria gruberi ckk (Complex from Naegleria gruberi)
- Tubulin (Complex from Homo sapiens)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6qvj
Q-score: 0.429
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Hsckk (human camsap1) decorated 14pf taxol-gdp microtubule (Complex)
- Calmodulin-regulated spectrin-associated protein 1 (19 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Camsap1 (Complex from Homo sapiens)
- Tubilin (Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J Struct Biol (2020) 209 pp. 107402-107402 [ DOI: doi:10.1016/j.jsb.2019.10.004 Pubmed: 31610239 ]
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J Struct Biol (2020) 209 pp. 107402-107402 [ DOI: doi:10.1016/j.jsb.2019.10.004 Pubmed: 31610239 ]