Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 6zpg
Q-score: 0.125
Atherton J, Hummel JJ, Olieric N, Locke J, Pena A, Rosenfeld SS, Steinmetz MO, Hoogenraad CC, Moores CA
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61481 Pubmed: 33252036 ]
- Kif-binding protein (71 kDa, Protein from Homo sapiens)
- Kinesin binding protein cryo-em density (72 kDa, Complex from Homo sapiens)
Kinesin binding protein complexed with Kif15 motor domain
Resolution: 6.9 Å
EM Method: Single-particle
Fitted PDBs: 6zph
Q-score: 0.175
Atherton J, Hummel JJ, Olieric N, Locke J, Pena A, Rosenfeld SS, Steinmetz MO, Hoogenraad CC, Moores CA
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61481 Pubmed: 33252036 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Kif-binding protein (71 kDa, Protein from Homo sapiens)
- Kinesin binding protein complexed with kif15 motor domain cryo-em density (72 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Kinesin-like protein kif15 (41 kDa, Protein from Homo sapiens)
Microtubule complexed with Kif15 motor domain. Symmetrised asymmetric unit
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6zpi
Q-score: 0.238
Atherton J, Hummel JJ, Olieric N, Locke J, Pena A, Rosenfeld SS, Steinmetz MO, Hoogenraad CC, Moores CA
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61481 Pubmed: 33252036 ]
- Kinesin-like protein kif15 (41 kDa, Protein from Homo sapiens)
- Microtubule (Complex from Sus scrofa)
- Tubulin alpha-1b chain (48 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kif15 motor domain (amppnp bound) complexed with microtubule. symmetrised asymmetric unit. (72 kDa, Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kif15 motor domain (Complex from Homo sapiens)
- Tubulin beta chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Taxol (853 Da, Ligand)
HsCKK (human CAMSAP1) decorated 13pf taxol-GDP microtubule
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6qus
Q-score: 0.454
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Calmodulin-regulated spectrin-associated protein 1 (Complex from Homo sapiens)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin (Complex from Homo sapiens)
- Calmodulin-regulated spectrin-associated protein 1 (19 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Hsckk (human camsap1) decorated 13pf taxol-gdp microtubule (Complex)
- Magnesium ion (24 Da, Ligand)
- Taxol (853 Da, Ligand)
NgCKK (N.Gruberi CKK) decorated 13pf taxol-GDP microtubule
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6quy
Q-score: 0.434
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Ngckk (n.gruberi ckk) decorated 13pf taxol-gdp microtubule (Complex)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin (Complex from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ckk domain protein (20 kDa, Protein from Naegleria gruberi)
- N.gruberi ckk (Complex from Naegleria gruberi)
- Taxol (853 Da, Ligand)
NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6qve
Q-score: 0.435
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Beta1-tubulin (49 kDa, Protein from Homo sapiens)
- Naegleria gruberi ckk (Complex from Naegleria gruberi)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin (Complex from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ngckk (naegleria gruberi ckk) decorated 14pf taxol-gdp microtubule (Complex)
- Magnesium ion (24 Da, Ligand)
- Predicted protein (20 kDa, Protein from Naegleria gruberi)
- Taxol (853 Da, Ligand)
HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6qvj
Q-score: 0.429
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun (2019) 10 pp. 5236-5236 [ Pubmed: 31748546 DOI: doi:10.1038/s41467-019-13247-6 ]
- Hsckk (human camsap1) decorated 14pf taxol-gdp microtubule (Complex)
- Tubilin (Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Camsap1 (Complex from Homo sapiens)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Calmodulin-regulated spectrin-associated protein 1 (19 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Taxol (853 Da, Ligand)
Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 5m5c
Q-score: 0.215
Atherton J, Jiang K, Stangier MM, Luo Y, Hua S, Houben K, van Hooff JJE, Joseph AP, Scarabelli G, Grant BJ, Roberts AJ, Topf M, Steinmetz MO, Baldus M, Moores CA, Akhmanova A
Nat Struct Mol Biol (2017) 24 pp. 931-943 [ DOI: doi:10.1038/nsmb.3483 Pubmed: 28991265 ]
- Calmodulin-regulated spectrin-associated protein 1 (13 kDa, Protein from Homo sapiens)
- Camsap1-n1492a mutant ckk domain (Complex from homo sapiens)
- Tubulin beta-2b chain (47 kDa, Protein from Bos taurus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Complex of two tubulin dimers with bound camsap1-n1492a mutant ckk domain (Complex)
- Magnesium ion (24 Da, Ligand)
- Tubulin dimer (Complex from Bos taurus)
- Water (18 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Bos taurus)
- Taxol (853 Da, Ligand)
Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins
Resolution: 5.3 Å
EM Method: Single-particle
Fitted PDBs: 5m50
Q-score: 0.175
Atherton J, Jiang K, Stangier MM, Luo Y, Hua S, Houben K, van Hooff JJE, Joseph AP, Scarabelli G, Grant BJ, Roberts AJ, Topf M, Steinmetz MO, Baldus M, Moores CA, Akhmanova A
Nat Struct Mol Biol (2017) 24 pp. 931-943 [ DOI: doi:10.1038/nsmb.3483 Pubmed: 28991265 ]
- Complex of two tubulin dimers with bound camsap3-ckk domain (Complex)
- Tubulin (Complex from Bos taurus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Tubulin beta-2b chain (47 kDa, Protein from Bos taurus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Camsap3-ckk domain (Complex from Mus musculus)
- Calmodulin-regulated spectrin-associated protein 3 (13 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Bos taurus)
Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 5m54
Q-score: 0.147
Atherton J, Jiang K, Stangier MM, Luo Y, Hua S, Houben K, van Hooff JJE, Joseph AP, Scarabelli G, Grant BJ, Roberts AJ, Topf M, Steinmetz MO, Baldus M, Moores CA, Akhmanova A
Nat Struct Mol Biol (2017) 24 pp. 931-943 [ DOI: doi:10.1038/nsmb.3483 Pubmed: 28991265 ]
- Camsap1-ckk domain (Complex from Homo sapiens)
- Tubulin beta-2b chain (47 kDa, Protein from Bos taurus)
- Calmodulin-regulated spectrin-associated protein 1 (13 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Complex of two tubulin dimers with bound camsap1-ckk domain (Complex)
- Tubulin (Complex from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Bos taurus)
- Taxol (853 Da, Ligand)