Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 6zpg
Q-score: 0.125
Atherton J, Hummel JJ, Olieric N, Locke J, Pena A, Rosenfeld SS, Steinmetz MO, Hoogenraad CC, Moores CA
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61481 Pubmed: 33252036 ]
- Kif-binding protein (71 kDa, Protein from Homo sapiens)
- Kinesin binding protein cryo-em density (72 kDa, Complex from Homo sapiens)
Kinesin binding protein complexed with Kif15 motor domain
Resolution: 6.9 Å
EM Method: Single-particle
Fitted PDBs: 6zph
Q-score: 0.175
Atherton J, Hummel JJ, Olieric N, Locke J, Pena A, Rosenfeld SS, Steinmetz MO, Hoogenraad CC, Moores CA
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61481 Pubmed: 33252036 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Kif-binding protein (71 kDa, Protein from Homo sapiens)
- Kinesin binding protein complexed with kif15 motor domain cryo-em density (72 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Kinesin-like protein kif15 (41 kDa, Protein from Homo sapiens)
Microtubule complexed with Kif15 motor domain. Symmetrised asymmetric unit
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6zpi
Q-score: 0.238
Atherton J, Hummel JJ, Olieric N, Locke J, Pena A, Rosenfeld SS, Steinmetz MO, Hoogenraad CC, Moores CA
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61481 Pubmed: 33252036 ]
- Kinesin-like protein kif15 (41 kDa, Protein from Homo sapiens)
- Microtubule (Complex from Sus scrofa)
- Tubulin alpha-1b chain (48 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kif15 motor domain (amppnp bound) complexed with microtubule. symmetrised asymmetric unit. (72 kDa, Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kif15 motor domain (Complex from Homo sapiens)
- Tubulin beta chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Taxol (853 Da, Ligand)
Cryo micrographs of microtubules (GDP state) decorated with NDC-NDC chimera of human doublecortin
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J. Struct. Biol. (2019) 209 [ Pubmed: 31610239 DOI: 10.1016/j.jsb.2019.10.004 ]
Image sets:
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6rf8
Q-score: 0.449
Cook AD, Manka SW, Wang S, Moores CA, Atherton J
J Struct Biol (2020) 209 pp. 107402-107402 [ Pubmed: 31610239 DOI: doi:10.1016/j.jsb.2019.10.004 ]
- Tubulin (Complex from Bos taurus)
- Tubulin alpha-1b chain (48 kDa, Protein from Bos taurus)
- Human doublecortin (Complex from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 5-component complex of n-terminal dc domain (ndc) of ndc-ndc chimera (human doublecortin sequence) bound to 13-protofilament gdp-microtubule lattice site composed of 4 tubulin subunits (two alpha-subunits and two beta-subunits) (Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Neuronal migration protein doublecortin (11 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta-2b chain (48 kDa, Protein from Bos taurus)