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author:"Beckert B" AND xref_links:"uniprot" AND author_name:"Chiba" AND xref_links:"cpx"
E. coli ApdP-stalled ribosomal complex
Release date: March 20, 2024
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8qbt
Q-score: 0.611
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8qbt
Q-score: 0.611
Morici M, Gabrielli S, Fujiwara K, Paternoga H, Beckert B, Bock LV, Chiba S, Wilson DN
Nat Commun (2024) 15 pp. 2432-2432 [ DOI: doi:10.1038/s41467-024-46761-3 Pubmed: 38503735 ]
Sample:
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s6, fully modified isoform (15 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl28 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl27 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli BW25113)
- E. coli apdp-stalled ribosomal complex (Complex from Sinorhizobium medicae)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli BW25113)
- 16s rrna (499 kDa, RNA from Escherichia coli BW25113)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli BW25113)
- Mrna (3 kDa, RNA from Sinorhizobium medicae)
- Apdp nascent chain (688 Da, Protein from Sinorhizobium medicae)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli BW25113)
- Ala-trna (24 kDa, RNA from Escherichia coli BW25113)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli BW25113)
- Proline (115 Da, Ligand)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli BW25113)
- 23s rrna (941 kDa, RNA from Escherichia coli BW25113)
- 5s rrna (38 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli BW25113)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl17 (14 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli BW25113)
- Pro-trna (24 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BW25113)
Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis
Release date: Dec. 4, 2019
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 6szs
Q-score: 0.535
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 6szs
Q-score: 0.535
Shimokawa-Chiba N, Muller C, Fujiwara K, Beckert B, Ito K, Wilson DN, Chiba S
Nat Commun (2019) 10 pp. 5397-5397 [ Pubmed: 31776341 DOI: doi:10.1038/s41467-019-13408-7 ]
Sample:
- Mrna (1 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
- Bacillus subtilis ribosome rescue factor (brfa; former yqkk) and peptide chain release factor 2 (rf2) (Complex from Bacillus subtilis (strain 168))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
- Uncharacterized protein yqkk (8 kDa, Protein from Bacillus subtilis (strain 168))
- E. coli 70s stalled on truncated mrna in complex bsbrfa and bsrf2-q251p (Complex)
- P-site trna-pro(cgg) (24 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- Escherichia coli ribosome with p-site trna-pro(cgg, carrying the nascent chain (Complex from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
- Peptide chain release factor 2 (41 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))