Cas nuclease-CRISPR (cr)RNA ribonucleoprotein (RNP) complex
Wandera KG, Schmelz S, Migur A, Kibe A, Lukat P, Achmedov T, Caliskan N, Blankenfeldt W, Beisel CL
Mol Cell (2025) [ DOI: doi:10.1016/j.molcel.2025.01.020 Pubmed: 39965569 ]
- Phage head-tail adaptor (134 kDa, Protein from Segatella buccae)
- Crrna (25 kDa, RNA from synthetic construct)
- Cas13b (cr)rna complex (Complex from Segatella buccae)
PbuCas13b-crRNA complex, open conformation
Wandera KG, Schmelz S, Migur A, Kibe A, Lukat P, Achmedov T, Caliskan N, Blankenfeldt W, Beisel CL
Mol Cell (2025) [ DOI: doi:10.1016/j.molcel.2025.01.020 Pubmed: 39965569 ]
- Cas13b (Protein from Segatella buccae)
- Crrna (RNA from synthetic construct)
- Cas13b (cr)rna complex (Complex from Segatella buccae)
PbuCas13b Helical-2 domain in complex with anti-CRISPR protein (AcrVIB1)
Wandera KG, Schmelz S, Migur A, Kibe A, Lukat P, Achmedov T, Caliskan N, Blankenfeldt W, Beisel CL
Mol Cell (2025) [ DOI: doi:10.1016/j.molcel.2025.01.020 Pubmed: 39965569 ]
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8qwd
Q-score: 0.413
Jabalera Y, Tascon I, Samperio S, Lopez-Alonso JP, Gonzalez-Lopez M, Aransay AM, Abascal-Palacios G, Beisel CL, Ubarretxena-Belandia I, Perez-Jimenez R
Nat Biotechnol (2024) [ DOI: doi:10.1038/s41587-024-02461-3 Pubmed: 39482449 ]
- Water (18 Da, Ligand)
- Apo rechb (Complex from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Rechb (148 kDa, Protein from synthetic construct)
Jabalera Y, Tascon I, Samperio S, Lopez-Alonso JP, Gonzalez-Lopez M, Aransay AM, Abascal-Palacios G, Beisel CL, Ubarretxena-Belandia I, Perez-Jimenez R
Nat Biotechnol (2024) [ DOI: doi:10.1038/s41587-024-02461-3 Pubmed: 39482449 ]
- Rechb (Protein from synthetic construct)
- Apo rechb (Complex from synthetic construct)
Ternary complex of ReChb - crRNA - target dsDNA
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8qwe
Q-score: 0.41
Jabalera Y, Tascon I, Samperio S, Lopez-Alonso JP, Gonzalez-Lopez M, Aransay AM, Abascal-Palacios G, Beisel CL, Ubarretxena-Belandia I, Perez-Jimenez R
Nat Biotechnol (2024) [ DOI: doi:10.1038/s41587-024-02461-3 Pubmed: 39482449 ]
- Crrna (12 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Ternary complex of rechb - crrna - target dsdna (Complex from synthetic construct)
- Rechb (148 kDa, Protein from synthetic construct)
- Target dna (11 kDa, DNA from synthetic construct)
- Non target dna (12 kDa, DNA from synthetic construct)
Quaternary complex of ReChb - crRNA - target dsDNA - collateral dsDNA
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8qwf
Q-score: 0.417
Jabalera Y, Tascon I, Samperio S, Lopez-Alonso JP, Gonzalez-Lopez M, Aransay AM, Abascal-Palacios G, Beisel CL, Ubarretxena-Belandia I, Perez-Jimenez R
Nat Biotechnol (2024) [ DOI: doi:10.1038/s41587-024-02461-3 Pubmed: 39482449 ]
- Quaternary complex of rechb - crrna - target dsdna - collateral dsdna (Complex from synthetic construct)
- Crrna (12 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Non target dna (11 kDa, DNA from synthetic construct)
- Rechb (148 kDa, Protein from synthetic construct)
- Target dna (12 kDa, DNA from synthetic construct)
Cryo-EM micrographs of anti-CRISPR associated protein Aca2 bound to its RNA
Birkholz N, Kamata K, Feussner M, Wilkinson ME, Cuba Samaniego C, Migur A, Kimanius D, Ceelen M, Went SC, Usher B, Blower TR, Brown CM, Beisel CL, Weinberg Z, Fagerlund RD, Jackson SA, Fineran PC
Nature (2024) 631 pp. 670-677 [ Pubmed: 38987591 DOI: 10.1038/s41586-024-07644-1 ]
Image sets:
Aca2 from Pectobacterium phage ZF40 bound to RNA
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8w35
Q-score: 0.558
Birkholz N, Kamata K, Feussner M, Wilkinson ME, Cuba Samaniego C, Migur A, Kimanius D, Ceelen M, Went SC, Usher B, Blower TR, Brown CM, Beisel CL, Weinberg Z, Fagerlund RD, Jackson SA, Fineran PC
Nature (2024) 631 pp. 670-677 [ DOI: doi:10.1038/s41586-024-07644-1 Pubmed: 38987591 ]
Structure of Cas12a2 binary complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8d49
Q-score: 0.5
Bravo JPK, Hallmark T, Naegle B, Beisel CL, Jackson RN, Taylor DW
Nature (2023) 613 pp. 582-587 [ DOI: doi:10.1038/s41586-022-05560-w Pubmed: 36599980 ]
- Rna (26-mer) (8 kDa, RNA from synthetic construct)
- Orfb_zn_ribbon domain-containing protein (143 kDa, Protein from Sulfuricurvum sp. PC08-66)
- Cas12a2 nuclease (Complex from Sulfuricurvum sp. PC08-66)