Full-length ClpX AAA protein in a complex with ClpP peptidase
C2-symmetric single-particle cryo-EM map of T. vaginalis FDPF3
Abdulaziz EN, Bell TA, Rashid B, Heacock ML, Begic T, Skinner OS, Yaseen MA, Chao LH, Mootha VK, Pierik AJ, Cracan V
J Biol Chem (2022) 298 pp. 102210-102210 [ Pubmed: 35780837 DOI: doi:10.1016/j.jbc.2022.102210 ]
C1-symmetric single-particle cryo-EM map of T. vaginalis FDPF3
Abdulaziz EN, Bell TA, Rashid B, Heacock ML, Begic T, Skinner OS, Yaseen MA, Chao LH, Mootha VK, Pierik AJ, Cracan V
J Biol Chem (2022) 298 pp. 102210-102210 [ Pubmed: 35780837 DOI: doi:10.1016/j.jbc.2022.102210 ]
ClpP and ClpX IGF loop in ClpX-ClpP complex bound to ssrA tagged GFP
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 6wr2
Q-score: 0.518
Fei X, Bell TA, Barkow SR, Baker TA, Sauer RT
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61496 Pubmed: 33089779 ]
- Water (18 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli (strain K12))
- Clpx-clpp-gfpssra-atp/atprs (Complex from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli (strain K12))
ClpX-ClpP complex bound to GFP-ssrA, recognition complex
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 6wrf
Q-score: 0.414
Fei X, Bell TA, Barkow SR, Baker TA, Sauer RT
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61496 Pubmed: 33089779 ]
- Green fluorescent protein (30 kDa, Protein from Aequorea victoria)
- Clpx-clpp-gfpssra-atp/atprs complex during target recognition (Complex from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli (strain K12))
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli (strain K12))
ClpX-ClpP complex bound to ssrA-tagged GFP, intermediate complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 6wsg
Q-score: 0.406
Fei X, Bell TA, Barkow SR, Baker TA, Sauer RT
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61496 Pubmed: 33089779 ]
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Water (18 Da, Ligand)
- Clpx-clpp-gfpssra-atp/atprs (Complex from Escherichia coli)
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Green fluorescent protein, + ssra tag (30 kDa, Protein from synthetic construct)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 4
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6po1
Q-score: 0.364
Fei X, Bell TA, Jenni S, Stinson BM, Baker TA, Harrison SC, Sauer RT
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52774 Pubmed: 32108573 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Substrate peptide (1 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease atp-binding subunit clpx (39 kDa, Protein from Escherichia coli)
- Clpx-clpp-substrate-atprs (Complex from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 3
Resolution: 4.28 Å
EM Method: Single-particle
Fitted PDBs: 6po3
Q-score: 0.345
Fei X, Bell TA, Jenni S, Stinson BM, Baker TA, Harrison SC, Sauer RT
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52774 Pubmed: 32108573 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (39 kDa, Protein from Escherichia coli)
- Clpx-clpp-substrate-atprs (Complex from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Substrate peptide (666 Da, Protein from Escherichia coli)
ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 2
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 6pod
Q-score: 0.368
Fei X, Bell TA, Jenni S, Stinson BM, Baker TA, Harrison SC, Sauer RT
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52774 Pubmed: 32108573 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (39 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Clpx-clpp-substrate-atprs (Complex from Escherichia coli)
- Substrate peptide (1 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)