Mechanosensitive channel MscS solubilized with LMNG in open conformation
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7onj
Q-score: 0.637
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Water (18 Da, Ligand)
- Homoheptameric complex of mscs coordinated with lipids and detergents ddm and lmng (224 kDa, Complex from Escherichia coli)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Mechanosensitive channel of small conductance (mscs) (31 kDa, Protein from Escherichia coli)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Mechanosensitive channel MscS solubilized with DDM in open conformation
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7oo0
Q-score: 0.559
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Mechanosensitive channel mscs (31 kDa, Protein from Escherichia coli)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Homoheptameric complex of mscs coordinated with lipids and detergent ddm (224 kDa, Complex from Escherichia coli)
Mechanosensitive channel MscS solubilized with LMNG in open conformation with added lipid
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7ooa
Q-score: 0.622
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Mechanosensitive channel of small conductance (mscs) (31 kDa, Protein from Escherichia coli SE11)
- Homoheptameric complex of mscs coordinated with lipids and detergents ddm and lmng (224 kDa, Complex from Escherichia coli SE11)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zyd
Q-score: 0.438
Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Bottcher B
PNAS (2020) 117 pp. 28754-28762 [ Pubmed: 33148804 DOI: doi:10.1073/pnas.2005641117 ]
YnaI in an open-like conformation
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6zye
Q-score: 0.388
Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Bottcher B
PNAS (2020) 117 pp. 28754-28762 [ Pubmed: 33148804 DOI: doi:10.1073/pnas.2005641117 ]
Structure of the ESX-3 core complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6sgw
Q-score: 0.427
Famelis N, Rivera-Calzada A, Degliesposti G, Wingender M, Mietrach N, Skehel JM, Fernandez-Leiro R, Bottcher B, Schlosser A, Llorca O, Geibel S
Nature (2019) 576 pp. 321-325 [ DOI: doi:10.1038/s41586-019-1633-1 Pubmed: 31597161 ]
- Esx-3 secretion system protein ecce3 (30 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Esx-3 secretion system atpase eccb3 (9 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Esx-3 secretion system protein eccd3 (47 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Esx-3 secretion system protein eccc3 (44 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Esx-3 core complex (650 kDa, Complex from Mycolicibacterium smegmatis MC2 155)