Cryo-EM structure of the DNA polymerase holoenzyme E9-A20-D4 of vaccinia virus
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8q3r
Q-score: 0.26
Burmeister WP, Boutin L, Balestra AC, Groger H, Ballandras-Colas A, Hutin S, Kraft C, Grimm C, Bottcher B, Fischer U, Tarbouriech N, Iseni F
PLoS Pathog (2024) 20 pp. e1011652-e1011652 [ Pubmed: 38768256 DOI: doi:10.1371/journal.ppat.1011652 ]
- Dna polymerase (120 kDa, Protein from Vaccinia virus Copenhagen)
- Uracil-dna glycosylase (27 kDa, Protein from Vaccinia virus Copenhagen)
- Dna polymerase processivity factor component opg148 (49 kDa, Protein from Vaccinia virus Copenhagen)
- E9-a20-d4 dna polymerase holoenzyme (196 MDa, Complex from Vaccinia virus)
Structure of the complete Vaccinia DNA-dependent RNA polymerase complex
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 6rfl
Q-score: 0.572
Grimm C, Hillen HS, Bedenk K, Bartuli J, Neyer S, Zhang Q, Huttenhofer A, Erlacher M, Dienemann C, Schlosser A, Urlaub H, Bottcher B, Szalay AA, Cramer P, Fischer U
Cell (2019) 179 pp. 1537-1550.e19 [ Pubmed: 31835032 DOI: doi:10.1016/j.cell.2019.11.024 ]
- Large subunit of mrna capping enzyme (96 kDa, Protein from Vaccinia virus GLV-1h68)
- Virion core protein (14 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo147 (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Complete vaccinia dna-dependent rna polymerase complex (Complex)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Chr17.trna16-glnttg (23 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Rna (Complex from Homo sapiens)
- Nucleoside triphosphate phosphohydrolase-i (72 kDa, Protein from Vaccinia virus GLV-1h68)
- Transcription factor vetf 82kda large subunit (82 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo132 (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia dna-dependent rna polymerase complex (Complex from Vaccinia virus GLV-1H68)
- Zinc ion (65 Da, Ligand)
- Putative h4l rna polymerase-associated transcription factor rap94 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo18 (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Water (18 Da, Ligand)
- Dna-dependent rna polymerase subunit rpo7 (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (30 kDa, Protein from Vaccinia virus GLV-1h68)
- Small subunit of mrna capping enzyme (33 kDa, Protein from Vaccinia virus GLV-1h68)
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Bottcher B, Norman DG, Owen-Hughes T
eLife (2017) 6 [ Pubmed: 28332978 DOI: doi:10.7554/eLife.22510 ]
- Chd1-nucleosome complex (400 kDa, Complex)
- Nucleosome (Complex from Xenopus laevis)
- Chromodomain-helicase-dna containing protein (Macromolecule)
- Chd1 (Complex from Saccharomyces cerevisiae)
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Bottcher B, Norman DG, Owen-Hughes T
eLife (2017) 6 [ Pubmed: 28332978 DOI: doi:10.7554/eLife.22510 ]
- Chd1 (Complex from Saccharomyces cerevisiae)
- Chd1-nucleosome complex (400 kDa, Complex)
- Chromodomain helicase dna binding protein (Macromolecule)
- Nucleosome (Complex from Xenopus laevis)