Mechanosensitive channel MscS solubilized with LMNG in open conformation
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7onj
Q-score: 0.637
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Water (18 Da, Ligand)
- Homoheptameric complex of mscs coordinated with lipids and detergents ddm and lmng (224 kDa, Complex from Escherichia coli)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Mechanosensitive channel of small conductance (mscs) (31 kDa, Protein from Escherichia coli)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Mechanosensitive channel MscS solubilized with DDM in closed conformation
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7onl
Q-score: 0.427
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
Mechanosensitive channel MscS solubilized with DDM in open conformation
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7oo0
Q-score: 0.559
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Mechanosensitive channel mscs (31 kDa, Protein from Escherichia coli)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Homoheptameric complex of mscs coordinated with lipids and detergent ddm (224 kDa, Complex from Escherichia coli)
Mechanosensitive channel MscS solubilized with DDM in closed conformation with added lipid
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7oo6
Q-score: 0.513
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Homoheptameric complex of mscs coordinated with lipids and detergent ddm (224 kDa, Complex from Escherichia coli)
- Mechanosensitive channel of small conductance (mscs) (31 kDa, Protein from Escherichia coli)
Mechanosensitive channel MscS solubilized with LMNG in closed conformation with added lipid
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7oo8
Q-score: 0.458
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
Mechanosensitive channel MscS solubilized with LMNG in open conformation with added lipid
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7ooa
Q-score: 0.622
Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B
PNAS (2021) 118 [ Pubmed: 34376558 DOI: doi:10.1073/pnas.2107095118 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Mechanosensitive channel of small conductance (mscs) (31 kDa, Protein from Escherichia coli SE11)
- Homoheptameric complex of mscs coordinated with lipids and detergents ddm and lmng (224 kDa, Complex from Escherichia coli SE11)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zyd
Q-score: 0.438
Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Bottcher B
PNAS (2020) 117 pp. 28754-28762 [ Pubmed: 33148804 DOI: doi:10.1073/pnas.2005641117 ]
YnaI in an open-like conformation
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6zye
Q-score: 0.388
Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Bottcher B
PNAS (2020) 117 pp. 28754-28762 [ Pubmed: 33148804 DOI: doi:10.1073/pnas.2005641117 ]
small conductance mechanosensitive channel YnaI mutant G149A/G152A
Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Bottcher B
PNAS (2020) 117 pp. 28754-28762 [ Pubmed: 33148804 DOI: doi:10.1073/pnas.2005641117 ]
mechanosensitive channel YnaI in amphipol A8-35
Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Bottcher B
PNAS (2020) 117 pp. 28754-28762 [ Pubmed: 33148804 DOI: doi:10.1073/pnas.2005641117 ]
- Ynai in amphipol a8-35 (278 kDa, Complex from Escherichia coli)
- Ynai (Protein from Escherichia coli)