Engineered disulfide cross-linked closed conformation of the Yeast gamma-TuRC(SS)
Resolution: 3.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2w
Q-score: 0.435
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Engineered disulfide cross-linked closed conformation of the yeast gamma-turc(ss) (Complex from Saccharomyces cerevisiae)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Open conformation of the Yeast wild-type gamma-TuRC
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2x
Q-score: 0.295
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Open conformation of the yeast wild-type gamma-turc (Complex from Saccharomyces cerevisiae)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Closed conformation of the Yeast wild-type gamma-TuRC
Resolution: 4.03 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2y
Q-score: 0.249
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Closed conformation of the yeast wild-type gamma-turc (Complex from Saccharomyces cerevisiae)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Monomeric single-particle reconstruction of the Yeast gamma-TuSC
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7m2z
Q-score: 0.314
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Monomer of the yeast gamma-tusc (300 kDa, Complex from Saccharomyces cerevisiae)
Single-particle reconstruction of a dimer of the Yeast gamma-TuSC
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
Human Tom70 in complex with SARS CoV2 Orf9b
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7kdt
Q-score: 0.473
Gordon DE, Hiatt J, Bouhaddou M, Rezelj VV, Ulferts S, Braberg H, Jureka AS, Obernier K, Guo JZ, Batra J, Kaake RM, Weckstein AR, Owens TW, Gupta M, Pourmal S, Titus EW, Cakir M, Soucheray M, McGregor M, Cakir Z, Jang G, O'Meara MJ, Tummino TA, Zhang Z, Foussard H, Rojc A, Zhou Y, Kuchenov D, Huttenhain R, Xu J, Eckhardt M, Swaney DL, Fabius JM, Ummadi M, Tutuncuoglu B, Rathore U, Modak M, Haas P, Haas KM, Naing ZZC, Pulido EH, Shi Y, Barrio-Hernandez I, Memon D, Petsalaki E, Dunham A, Marrero MC, Burke D, Koh C, Vallet T, Silvas JA, Azumaya CM, Billesbolle C, Brilot AF, Campbell MG, Diallo A, Dickinson MS, Diwanji D, Herrera N, Hoppe N, Kratochvil HT, Liu Y, Merz GE, Moritz M, Nguyen HC, Nowotny C, Puchades C, Rizo AN, Schulze-Gahmen U, Smith AM, Sun M, Young ID, Zhao J, Asarnow D, Biel J, Bowen A, Braxton JR, Chen J, Chio CM, Chio US, Deshpande I, Doan L, Faust B, Flores S, Jin M, Kim K, Lam VL, Li F, Li J, Li YL, Li Y, Liu X, Lo M, Lopez KE, Melo AA, Moss 3rd FR, Nguyen P, Paulino J, Pawar KI, Peters JK, Pospiech Jr TH, Safari M, Sangwan S, Schaefer K, Thomas PV, Thwin AC, Trenker R, Tse E, Tsui TKM, Wang F, Whitis N, Yu Z, Zhang K, Zhang Y, Zhou F, Saltzberg D, Hodder AJ, Shun-Shion AS, Williams DM, White KM, Rosales R, Kehrer T, Miorin L, Moreno E, Patel AH, Rihn S, Khalid MM, Vallejo-Gracia A, Fozouni P, Simoneau CR, Roth TL, Wu D, Karim MA, Ghoussaini M, Dunham I, Berardi F, Weigang S, Chazal M, Park J, Logue J, McGrath M, Weston S, Haupt R, Hastie CJ, Elliott M, Brown F, Burness KA, Reid E, Dorward M, Johnson C, Wilkinson SG, Geyer A, Giesel DM, Baillie C, Raggett S, Leech H, Toth R, Goodman N, Keough KC, Lind AL, Klesh RJ, Hemphill KR, Carlson-Stevermer J, Oki J, Holden K, Maures T, Pollard KS, Sali A, Agard DA, Cheng Y, Fraser JS, Frost A, Jura N, Kortemme T, Manglik A, Southworth DR, Stroud RM, Alessi DR, Davies P, Frieman MB, Ideker T, Abate C, Jouvenet N, Kochs G, Shoichet B, Ott M, Palmarini M, Shokat KM, Garcia-Sastre A, Rassen JA, Grosse R, Rosenberg OS, Verba KA, Basler CF, Vignuzzi M, Peden AA, Beltrao P, Krogan NJ
Science (2020) 370 [ DOI: doi:10.1126/science.abe9403 Pubmed: 33060197 ]
- Orf9b-tom70 complex (Complex from Homo sapiens)
- Mitochondrial import receptor subunit tom70 (56 kDa, Protein from Homo sapiens)
- Orf9b protein (10 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)