Engineered disulfide cross-linked closed conformation of the Yeast gamma-TuRC(SS)
Resolution: 3.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2w
Q-score: 0.435
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Engineered disulfide cross-linked closed conformation of the yeast gamma-turc(ss) (Complex from Saccharomyces cerevisiae)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Open conformation of the Yeast wild-type gamma-TuRC
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2x
Q-score: 0.295
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Open conformation of the yeast wild-type gamma-turc (Complex from Saccharomyces cerevisiae)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Closed conformation of the Yeast wild-type gamma-TuRC
Resolution: 4.03 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2y
Q-score: 0.249
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Closed conformation of the yeast wild-type gamma-turc (Complex from Saccharomyces cerevisiae)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Monomeric single-particle reconstruction of the Yeast gamma-TuSC
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7m2z
Q-score: 0.314
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Monomer of the yeast gamma-tusc (300 kDa, Complex from Saccharomyces cerevisiae)