HIV BG505.v5.2 (N289/N241) SOSIP Env in Complex with V5 pAb from Rh.33203
Brown S, Antanasijevic A, Sewall LM, Garcia DM, Brouwer PJM, Sanders RW, Ward AB
Sci Immunol (2025) 10 pp. eadp5218-eadp5218 [ Pubmed: 39823319 DOI: doi:10.1126/sciimmunol.adp5218 ]
- Hiv-1 bg505 sosip env in complex with nhp polyclonal antibody - v5 epitope (Complex from Macaca mulatta)
- Nhp polyclonal antibody v5 epitope - predicted light chain (8 kDa, Protein from Macaca mulatta)
- Nhp polyclonal antibody v5 epitope - predicted heavy chain (9 kDa, Protein from Macaca mulatta)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Transmembrane protein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Surface protein gp120 (57 kDa, Protein from Human immunodeficiency virus 1)
Brown S, Antanasijevic A, Sewall LM, Garcia DM, Brouwer PJM, Sanders RW, Ward AB
Sci Immunol (2025) 10 pp. eadp5218-eadp5218 [ Pubmed: 39823319 DOI: doi:10.1126/sciimmunol.adp5218 ]
- Nhp v5 epitope pab - predicted light chain (8 kDa, Protein from Macaca mulatta)
- Nhp v5 epitope pab - predicted heavy chain (9 kDa, Protein from Macaca mulatta)
- Hiv-1 bg505 sosip env in complex with nhp polyclonal antibody - v5, gp120-interface, and anti-immune complex epitopes (Complex from Macaca mulatta)
- Nhp anti-immune complex pab - predicted heavy chain (8 kDa, Protein from Macaca mulatta)
- Nhp gp120-interface epitope pab - predicted light chain (8 kDa, Protein from Macaca mulatta)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Transmembrane protein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Surface protein gp120 (57 kDa, Protein from Human immunodeficiency virus 1)
- Nhp gp120-interface epitope pab - predicted heavy chain (10 kDa, Protein from Macaca mulatta)
- Nhp anti-immune complex pab - predicted light chain (8 kDa, Protein from Macaca mulatta)
Brown S, Antanasijevic A, Sewall LM, Garcia DM, Brouwer PJM, Sanders RW, Ward AB
Sci Immunol (2025) 10 pp. eadp5218-eadp5218 [ Pubmed: 39823319 DOI: doi:10.1126/sciimmunol.adp5218 ]
- Rabbit v1/v3 epitope pab - predicted heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- Surface protein gp120 (56 kDa, Protein from Human immunodeficiency virus 1)
- Rabbit anti-immune complex pab - predicted light chain (8 kDa, Protein from Oryctolagus cuniculus)
- Rabbit v1/v3 epitope pab - predicted light chain (8 kDa, Protein from Oryctolagus cuniculus)
- Transmembrane protein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Hiv ch505/bg505 sosip env in complex with v1/v3 and anti-immune complex pabs (Complex from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Lassa virus glycoprotein construct (Josiah GPC-I53-50A) in complex with LAVA01 antibody
Resolution: 4.41 Å
EM Method: Single-particle
Fitted PDBs: 7sgf
Q-score: 0.292
Brouwer PJM, Antanasijevic A, Ronk AJ, Muller-Krauter H, Watanabe Y, Claireaux M, Perrett HR, Bijl TPL, Grobben M, Umotoy JC, Schriek AI, Burger JA, Tejjani K, Lloyd NM, Steijaert TH, van Haaren MM, Sliepen K, de Taeye SW, van Gils MJ, Crispin M, Strecker T, Bukreyev A, Ward AB, Sanders RW
Cell Host Microbe (2022) 30 pp. 1759- [ DOI: doi:10.1016/j.chom.2022.10.018 Pubmed: 36400021 ]
- Gpc-i53-50a (73 kDa, Protein from Lassa mammarenavirus)
- Lava01 mab - heavy chain (15 kDa, Protein from Oryctolagus cuniculus)
- Lava01 mab - light chain (12 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lassa virus glycoprotein construct(josiah gpc-i53-50a) in complex with lava01 antibody (as fab fragment) (Complex from Lassa mammarenavirus)
Resolution: 4.25 Å
EM Method: Single-particle
Fitted PDBs: 6vfk
Q-score: 0.316
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
- T33_dn10b (31 kDa, Protein from synthetic construct)
- De novo designed tetrahedral nanoparticle t33_dn10 displaying 4 copies of bg505-sosip trimer on the surface. nanoparticle consists of 12 copies of both subunits (bg505-sosip-t33_dn10a and t33_dn10b) (Complex from synthetic construct)
- Bg505-sosip-t33_dn10a (88 kDa, Protein from synthetic construct)
Resolution: 4.14 Å
EM Method: Single-particle
Fitted PDBs: 6vfl
Q-score: 0.404
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
- Bg505-sosipv5.2(7s) - gp120 (56 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg505-sosipv5.2(7s) - gp41 (17 kDa, Protein from synthetic construct)
- Bg505-sosip reconstructed from the tetrahedral nanoparticle t33_dn10 by subparticle extraction and refinement (Complex from synthetic construct)
Single Particle Electron Cryo-Microscopy Reconstruction of BG505 SOSIP-I53-50NP
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6p6f
Q-score: 0.301
Brouwer PJM, Antanasijevic A, Berndsen Z, Yasmeen A, Fiala B, Bijl TPL, Bontjer I, Bale JB, Sheffler W, Allen JD, Schorcht A, Burger JA, Camacho M, Ellis D, Cottrell CA, Behrens AJ, Catalano M, Del Moral-Sanchez I, Ketas TJ, LaBranche C, van Gils MJ, Sliepen K, Stewart LJ, Crispin M, Montefiori DC, Baker D, Moore JP, Klasse PJ, Ward AB, King NP, Sanders RW
Nat Commun (2019) 10 pp. 4272-4272 [ DOI: doi:10.1038/s41467-019-12080-1 Pubmed: 31537780 ]
- I53-50b.4pt1 (18 kDa, Protein from synthetic construct)
- I53-50b.4pt1 (Complex from synthetic construct)
- Two-component self-assembling nanoparticle consisting of bg505 sosip-i53-50a.1nt1 and i53-50b.4pt1 (Complex)
- I53-50a.1nt1 (99 kDa, Protein from synthetic construct)
- I53-50a.1nt1 (Complex from synthetic construct)