HIV-1 B41 SOSIP.v4.1 Env in Complex with N241 Epitope and Anti-Immune Complex Antibodies
Brown S, Antanasijevic A, Sewall LM, Garcia DM, Brouwer PJM, Sanders RW, Ward AB
Sci Immunol (2025) 10 pp. eadp5218-eadp5218 [ Pubmed: 39823319 DOI: doi:10.1126/sciimmunol.adp5218 ]
Brown S, Antanasijevic A, Sewall LM, Garcia DM, Brouwer PJM, Sanders RW, Ward AB
Sci Immunol (2025) 10 pp. eadp5218-eadp5218 [ Pubmed: 39823319 DOI: doi:10.1126/sciimmunol.adp5218 ]
Structure of lineage IV Lassa virus glycoprotein complex (strain Josiah)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8ejd
Q-score: 0.373
Perrett HR, Brouwer PJM, Hurtado J, Newby ML, Liu L, Muller-Krauter H, Muller Aguirre S, Burger JA, Bouhuijs JH, Gibson G, Messmer T, Schieffelin JS, Antanasijevic A, Boons GJ, Strecker T, Crispin M, Sanders RW, Briney B, Ward AB
Cell Rep (2023) 42 pp. 112524-112524 [ Pubmed: 37209096 DOI: doi:10.1016/j.celrep.2023.112524 ]
- Glycoprotein g1 (29 kDa, Protein from Lassa mammarenavirus)
- Lassa mammarenavirus gpc (275 kDa, Complex from Lassa mammarenavirus)
- Glycoprotein g2 (44 kDa, Protein from Lassa mammarenavirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of lineage VII Lassa virus glycoprotein complex (strain Togo/2016/7082)
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8ejg
Q-score: 0.429
Perrett HR, Brouwer PJM, Hurtado J, Newby ML, Liu L, Muller-Krauter H, Muller Aguirre S, Burger JA, Bouhuijs JH, Gibson G, Messmer T, Schieffelin JS, Antanasijevic A, Boons GJ, Strecker T, Crispin M, Sanders RW, Briney B, Ward AB
Cell Rep (2023) 42 pp. 112524-112524 [ Pubmed: 37209096 DOI: doi:10.1016/j.celrep.2023.112524 ]
- Glycoprotein gp1 (22 kDa, Protein from Lassa mammarenavirus)
- Lassa mammarenavirus gpc (275 kDa, Complex from Lassa mammarenavirus)
- Glycoprotein gp2 (19 kDa, Protein from Lassa mammarenavirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Lassa virus glycoprotein complex (Josiah) bound to 12.1F Fab
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 8ejh
Q-score: 0.406
Perrett HR, Brouwer PJM, Hurtado J, Newby ML, Liu L, Muller-Krauter H, Muller Aguirre S, Burger JA, Bouhuijs JH, Gibson G, Messmer T, Schieffelin JS, Antanasijevic A, Boons GJ, Strecker T, Crispin M, Sanders RW, Briney B, Ward AB
Cell Rep (2023) 42 pp. 112524-112524 [ Pubmed: 37209096 DOI: doi:10.1016/j.celrep.2023.112524 ]
- Lassa mammarenavirus gpc (425 kDa, Complex from Lassa mammarenavirus)
- Alpha-d-mannopyranose (180 Da, Ligand)
- 12.1f fab light chain (11 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa mammarenavirus)
- Glycoprotein g2 (44 kDa, Protein from Lassa mammarenavirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 12.1f fab heavy chain (13 kDa, Protein from Homo sapiens)
Lassa virus glycoprotein complex (Josiah) bound to S370.7 Fab
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8ejj
Q-score: 0.506
Perrett HR, Brouwer PJM, Hurtado J, Newby ML, Liu L, Muller-Krauter H, Muller Aguirre S, Burger JA, Bouhuijs JH, Gibson G, Messmer T, Schieffelin JS, Antanasijevic A, Boons GJ, Strecker T, Crispin M, Sanders RW, Briney B, Ward AB
Cell Rep (2023) 42 pp. 112524-112524 [ Pubmed: 37209096 DOI: doi:10.1016/j.celrep.2023.112524 ]
- S370.7 light chain fab (11 kDa, Protein from Homo sapiens)
- Glycoprotein gp1 (22 kDa, Protein from Lassa mammarenavirus)
- Lassa mammarenavirus gpc (425 kDa, Complex from Lassa mammarenavirus)
- Alpha-d-mannopyranose (180 Da, Ligand)
- S370.7 heavy chain fab (14 kDa, Protein from Homo sapiens)
- Glycoprotein gp2 (19 kDa, Protein from Lassa mammarenavirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
De novo designed icosahedral nanoparticle I53_dn5 presenting BG505-SOSIP, Cryo-EM Map
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
- I53_dn5a (Protein from synthetic construct)
- Bg505-sosip-i53_dn5b (Protein from synthetic construct)
- De novo designed icosahedral nanoparticle i53_dn5 displaying 20 copies of bg505-sosip trimer on the surface. nanoparticle consists of 60 copies of both subunits (bg505-sosip-i53_dn5b and i53_dn5a) (Complex from synthetic construct)
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
Resolution: 4.25 Å
EM Method: Single-particle
Fitted PDBs: 6vfk
Q-score: 0.316
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
- T33_dn10b (31 kDa, Protein from synthetic construct)
- De novo designed tetrahedral nanoparticle t33_dn10 displaying 4 copies of bg505-sosip trimer on the surface. nanoparticle consists of 12 copies of both subunits (bg505-sosip-t33_dn10a and t33_dn10b) (Complex from synthetic construct)
- Bg505-sosip-t33_dn10a (88 kDa, Protein from synthetic construct)
Resolution: 4.14 Å
EM Method: Single-particle
Fitted PDBs: 6vfl
Q-score: 0.404
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
- Bg505-sosipv5.2(7s) - gp120 (56 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg505-sosipv5.2(7s) - gp41 (17 kDa, Protein from synthetic construct)
- Bg505-sosip reconstructed from the tetrahedral nanoparticle t33_dn10 by subparticle extraction and refinement (Complex from synthetic construct)