Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
Lineage IV Lassa virus glycoprotein (Josiah) in complex with monoclonal antibody 8.9F
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cj7
Q-score: 0.512
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Lineage iv lassa virus glycoprotein (josiah) in complex with monoclonal antibody 8.9f (Complex from Oryctolagus cuniculus)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Fv region of 8.9f heavy chain (31 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
- Fv region of 8.9f light chain (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Lassa GPC (strain Josiah) bound to rabbit polyclonal base-targeting antibody Base-1
Brouwer PJM, Perrett HR, Nijhuis H, Lee W-H, Burger J, Lloyd NM, Himansu S, Beaumont T, Carfi A, Bukreyev A, Sanders RW, van Gils MJ, Ward AB
To Be Published
- Glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Polyclonal antibody base-1 heavy chain, polyclonal antibody base-1 light chain (Complex from Oryctolagus cuniculus)
- Polyclonal antibody base-1 heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- Polyclonal antibody base-1 light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus)
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal base-targeting antibody base-1 (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 7sgd
Q-score: 0.366
Brouwer PJM, Antanasijevic A, Ronk AJ, Muller-Krauter H, Watanabe Y, Claireaux M, Perrett HR, Bijl TPL, Grobben M, Umotoy JC, Schriek AI, Burger JA, Tejjani K, Lloyd NM, Steijaert TH, van Haaren MM, Sliepen K, de Taeye SW, van Gils MJ, Crispin M, Strecker T, Bukreyev A, Ward AB, Sanders RW
Cell Host Microbe (2022) 30 pp. 1759- [ DOI: doi:10.1016/j.chom.2022.10.018 Pubmed: 36400021 ]
I53-50 nanoparticle core reconstructed from GPC-I53-50NP by focused refinement
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 7sge
Q-score: 0.479
Brouwer PJM, Antanasijevic A, Ronk AJ, Muller-Krauter H, Watanabe Y, Claireaux M, Perrett HR, Bijl TPL, Grobben M, Umotoy JC, Schriek AI, Burger JA, Tejjani K, Lloyd NM, Steijaert TH, van Haaren MM, Sliepen K, de Taeye SW, van Gils MJ, Crispin M, Strecker T, Bukreyev A, Ward AB, Sanders RW
Cell Host Microbe (2022) 30 pp. 1759- [ DOI: doi:10.1016/j.chom.2022.10.018 Pubmed: 36400021 ]
Lassa virus glycoprotein construct (Josiah GPC-I53-50A) in complex with LAVA01 antibody
Resolution: 4.41 Å
EM Method: Single-particle
Fitted PDBs: 7sgf
Q-score: 0.292
Brouwer PJM, Antanasijevic A, Ronk AJ, Muller-Krauter H, Watanabe Y, Claireaux M, Perrett HR, Bijl TPL, Grobben M, Umotoy JC, Schriek AI, Burger JA, Tejjani K, Lloyd NM, Steijaert TH, van Haaren MM, Sliepen K, de Taeye SW, van Gils MJ, Crispin M, Strecker T, Bukreyev A, Ward AB, Sanders RW
Cell Host Microbe (2022) 30 pp. 1759- [ DOI: doi:10.1016/j.chom.2022.10.018 Pubmed: 36400021 ]
- Gpc-i53-50a (73 kDa, Protein from Lassa mammarenavirus)
- Lava01 mab - heavy chain (15 kDa, Protein from Oryctolagus cuniculus)
- Lava01 mab - light chain (12 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lassa virus glycoprotein construct(josiah gpc-i53-50a) in complex with lava01 antibody (as fab fragment) (Complex from Lassa mammarenavirus)
Single Particle Electron Cryo-Microscopy Reconstruction of BG505 SOSIP-I53-50NP
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6p6f
Q-score: 0.301
Brouwer PJM, Antanasijevic A, Berndsen Z, Yasmeen A, Fiala B, Bijl TPL, Bontjer I, Bale JB, Sheffler W, Allen JD, Schorcht A, Burger JA, Camacho M, Ellis D, Cottrell CA, Behrens AJ, Catalano M, Del Moral-Sanchez I, Ketas TJ, LaBranche C, van Gils MJ, Sliepen K, Stewart LJ, Crispin M, Montefiori DC, Baker D, Moore JP, Klasse PJ, Ward AB, King NP, Sanders RW
Nat Commun (2019) 10 pp. 4272-4272 [ DOI: doi:10.1038/s41467-019-12080-1 Pubmed: 31537780 ]
- I53-50b.4pt1 (18 kDa, Protein from synthetic construct)
- I53-50b.4pt1 (Complex from synthetic construct)
- Two-component self-assembling nanoparticle consisting of bg505 sosip-i53-50a.1nt1 and i53-50b.4pt1 (Complex)
- I53-50a.1nt1 (99 kDa, Protein from synthetic construct)
- I53-50a.1nt1 (Complex from synthetic construct)