The 20S supercomplex engaging the SNAP-25 N-terminus (class 1)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6mdm
Q-score: 0.207
White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT
eLife (2018) 7 [ DOI: doi:10.7554/eLife.38888 Pubmed: 30198481 ]
- N-ethylmaleimide sensitive factor (Complex from Cricetulus griseus)
- Syntaxin-1a (29 kDa, Protein from Rattus norvegicus)
- Vesicle-associated membrane protein 2 (12 kDa, Protein from Rattus norvegicus)
- Alpha-soluble nsf attachment protein (35 kDa, Protein from Rattus norvegicus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Vesicle-fusing atpase (85 kDa, Protein from Cricetulus griseus)
- Alpha-soluble nsf attachment protein (Complex from Rattus norvegicus)
- Syntaxin-1a (Complex from Rattus norvegicus)
- Synaptosomal-associated protein 25 (23 kDa, Protein from Rattus norvegicus)
- 20s supercomplex consisting of soluble neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (33 kDa, Complex)
- Vesicle-associated membrane protein 2 (Complex from Rattus norvegicus)
- Synaptosomal-associated protein 25 (Complex from Rattus norvegicus)
The 20S supercomplex engaging the SNAP-25 N-terminus (class 2)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6mdn
Q-score: 0.213
White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT
eLife (2018) 7 [ DOI: doi:10.7554/eLife.38888 Pubmed: 30198481 ]
- N-ethylmaleimide sensitive factor (496 kDa, Complex from Cricetulus griseus)
- Syntaxin-1a (29 kDa, Protein from Rattus norvegicus)
- Syntaxin-1a (30 kDa, Complex from Rattus norvegicus)
- Vesicle-associated membrane protein 2 (12 kDa, Protein from Rattus norvegicus)
- Alpha-soluble nsf attachment protein (35 kDa, Protein from Rattus norvegicus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Synaptosomal-associated protein 25 (23 kDa, Complex from Rattus norvegicus)
- Vesicle-fusing atpase (85 kDa, Protein from Cricetulus griseus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 20s supercomplex consisting of soluble neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (628 kDa, Complex)
- Alpha-soluble nsf attachment protein (33 kDa, Complex from Rattus norvegicus)
- Vesicle-associated membrane protein 2 (10 kDa, Complex from Rattus norvegicus)
- Synaptosomal-associated protein 25 (23 kDa, Protein from Rattus norvegicus)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6mdo
Q-score: 0.32
White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT
eLife (2018) 7 [ DOI: doi:10.7554/eLife.38888 Pubmed: 30198481 ]
- 20s supercomplex consisting of soluble neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (23 kDa, Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Vesicle-fusing atpase (85 kDa, Protein from Cricetulus griseus)
- N-ethylmaleimide sensitive factor (Complex from Rattus norvegicus)
- Synaptosomal-associated protein 25 (23 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (Complex from Rattus norvegicus)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6mdp
Q-score: 0.316
White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT
eLife (2018) 7 [ DOI: doi:10.7554/eLife.38888 Pubmed: 30198481 ]
- 20s supercomplex consisting of soluble neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (23 kDa, Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Vesicle-fusing atpase (85 kDa, Protein from Cricetulus griseus)
- N-ethylmaleimide sensitive factor (Complex from Rattus norvegicus)
- Synaptosomal-associated protein 25 (23 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (Complex from Rattus norvegicus)
Choi UB, Zhao M, White KI, Pfuetzner RA, Esquivies L, Zhou Q, Brunger AT
eLife (2018) 7 [ DOI: doi:10.7554/eLife.36497 Pubmed: 29985126 ]
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 3j94
Q-score: 0.255
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- Atp-bound n-ethylmaleimide sensitive factor (500 kDa, Sample)
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
Resolution: 7.6 Å
EM Method: Single-particle
Fitted PDBs: 3j95
Q-score: 0.159
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- Adp-bound n-ethylmaleimide sensitive factor (500 kDa, Sample)
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state I
Resolution: 7.6 Å
EM Method: Single-particle
Fitted PDBs: 3j96
Q-score: 0.14
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
- 20s supercomplex consisting of truncated neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (660 kDa, Sample)
- Synaptobrevin-2 (8 kDa, Protein from Rattus norvegicus)
- Syntaxin-1a (8 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (16 kDa, Protein from Rattus norvegicus)
- Alpha soluble nsf attachment protein (33 kDa, Protein from Rattus norvegicus)
Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state II
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 3j97
Q-score: 0.136
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
- 20s supercomplex consisting of truncated neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (660 kDa, Sample)
- Synaptobrevin-2 (8 kDa, Protein from Rattus norvegicus)
- Syntaxin-1a (8 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (16 kDa, Protein from Rattus norvegicus)
- Alpha soluble nsf attachment protein (33 kDa, Protein from Rattus norvegicus)
Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state IIIa
Resolution: 8.4 Å
EM Method: Single-particle
Fitted PDBs: 3j98
Q-score: 0.124
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
- 20s supercomplex consisting of truncated neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (660 kDa, Sample)
- Synaptobrevin-2 (8 kDa, Protein from Rattus norvegicus)
- Syntaxin-1a (8 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (16 kDa, Protein from Rattus norvegicus)
- Alpha soluble nsf attachment protein (33 kDa, Protein from Rattus norvegicus)