Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state IIIa
Resolution: 8.4 Å
EM Method: Single-particle
Fitted PDBs: 3j98
Q-score: 0.124
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
- 20s supercomplex consisting of truncated neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (660 kDa, Sample)
- Synaptobrevin-2 (8 kDa, Protein from Rattus norvegicus)
- Syntaxin-1a (8 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (16 kDa, Protein from Rattus norvegicus)
- Alpha soluble nsf attachment protein (33 kDa, Protein from Rattus norvegicus)
Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state IIIb
Resolution: 8.2 Å
EM Method: Single-particle
Fitted PDBs: 3j99
Q-score: 0.133
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
- 20s supercomplex consisting of truncated neuronal snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (660 kDa, Sample)
- Synaptobrevin-2 (8 kDa, Protein from Rattus norvegicus)
- Syntaxin-1a (8 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (16 kDa, Protein from Rattus norvegicus)
- Alpha soluble nsf attachment protein (33 kDa, Protein from Rattus norvegicus)
Structure of 20S supercomplex with V7-SNARE determined by single particle cryoelectron microscopy
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature (2015) 518 pp. 61-67 [ Pubmed: 25581794 DOI: doi:10.1038/nature14148 ]
- Vesicle-associated membrane protein 7 (22 kDa, Protein from Rattus norvegicus)
- N-ethylmaleimide sensitive factor (83 kDa, Protein from Cricetulus griseus)
- Alpha soluble nsf attachment protein (33 kDa, Protein from Rattus norvegicus)
- Synaptosomal-associated protein 25 (25 kDa, Protein from Rattus norvegicus)
- Syntaxin-1a (30 kDa, Protein from Rattus norvegicus)
- 20s supercomplex consisting of vamp-7 snare complex, alpha-snap, and n-ethylmaleimide sensitive factor (nsf) (Sample)
Electron cryomicroscopy structure of N-ethyl maleimide sensitive factor at 11 A resolution.
Furst J, Sutton RB, Chen J, Brunger AT, Grigorieff N
EMBO J (2003) 22 pp. 4365-4374 [ DOI: doi:10.1093/emboj/cdg420 Pubmed: 12941689 ]
- Soluble nsf attachment protein (33 kDa, Protein from Bos taurus)
- Nsf alpha-snap snare 20s (680 kDa, Sample)
- N-ethyl maleimide sensitive factor (82 kDa, Protein from Cricetulus griseus)
- Soluble nsf attachment protein receptor (82 kDa, Protein from unidentified)