Qureshi BM, Behrmann E, Schoneberg J, Loerke J, Burger J, Mielke T, Giesebrecht J, Noe F, Lamb TD, Hofmann KP, Spahn CMT, Heck M
Open Biol (2018) 8 [ Pubmed: 30068566 DOI: doi:10.1098/rsob.180075 ]
map of the RNA polymerase lambda-based antitermination complex solved by cryo-EM
Resolution: 9.8 Å
EM Method: Single-particle
Fitted PDBs: 5ms0
Q-score: 0.069
Said N, Krupp F, Anedchenko E, Santos KF, Dybkov O, Huang YH, Lee CT, Loll B, Behrmann E, Burger J, Mielke T, Loerke J, Urlaub H, Spahn CMT, Weber G, Wahl MC
Nat Microbiol (2017) 2 pp. 17062-17062 [ DOI: doi:10.1038/nmicrobiol.2017.62 Pubmed: 28452979 ]
- Dnaii (11 kDa, DNA from Escherichia coli)
- Rna transcription bubble (4 kDa, RNA from Escherichia coli)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli K-12)
- Transcription antitermination protein nusb (15 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Lambdan-dependent antitermination complex (Complex from Escherichia coli)
- Nascent rna (9 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Antitermination protein n (9 kDa, Protein from Escherichia phage lambda)
- Dnai (8 kDa, DNA from Escherichia coli)
Cryo electron microscopy of actively translating human polysomes (POST state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]
- 80s ribosomal complex (4 MDa, Complex from Homo sapiens)
- Native ribosomal complex from polysomes - post state (4 MDa, Sample)
Cryo electron microscopy of actively translating human polysomes (POST-i2 state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]
- Native ribosomal complex from polysomes - post-i2 state (4 MDa, Sample)
- 80s ribosomal complex (4 MDa, Complex from Homo sapiens)
Cryo electron microscopy of actively translating human polysomes (POST-i3 state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]
- Native ribosomal complex from polysomes - post-i3 state (4 MDa, Sample)
- 80s ribosomal complex (4 MDa, Complex from Homo sapiens)
Cryo electron microscopy of actively translating human polysomes (rotated-1 PRE state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]
- Native ribosomal complex from polysomes - rotated-1 pre state (4 MDa, Sample)
- 80s ribosomal complex (4 MDa, Complex from Homo sapiens)
Cryo electron microscopy of actively translating human polysomes (rotated-2 PRE state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]
- 80s ribosomal complex (4 MDa, Complex from Homo sapiens)
- Native ribosomal complex from polysomes - rotated-2 pre state (4 MDa, Sample)
Cryo electron microscopy of actively translating human polysomes (PRE* state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]
- Native ribosomal complex from polysomes - pre* state (4 MDa, Sample)
- 80s ribosomal complex (4 MDa, Complex from Homo sapiens)
Cryo electron microscopy of actively translating human polysomes (classical iPRE state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]
- 80s ribosomal complex (4 MDa, Complex from Homo sapiens)
- Native ribosomal complex from polysomes - classical ipre state (4 MDa, Sample)
Cryo electron microscopy of actively translating human polysomes (decoding/post-hydrolysis state).
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CMT
Cell (2015) 161 pp. 845-857 [ Pubmed: 25957688 DOI: doi:10.1016/j.cell.2015.03.052 ]