Cryo-EM structure of RCMV-E E27 bound to human DDB1 (deltaBPB) and rat STAT2 CCD
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7zn7
Q-score: 0.395
Le-Trilling VTK, Banchenko S, Paydar D, Leipe PM, Binting L, Lauer S, Graziadei A, Klingen R, Gotthold C, Burger J, Bracht T, Sitek B, Jan Lebbink R, Malyshkina A, Mielke T, Rappsilber J, Spahn CM, Voigt S, Trilling M, Schwefel D
EMBO J (2023) 42 pp. e112351-e112351 [ DOI: doi:10.15252/embj.2022112351 Pubmed: 36762436 ]
- Signal transducer and activator of transcription (Complex from Rattus norvegicus)
- Dna damage-binding protein 1 (Complex from Homo sapiens)
- Signal transducer and activator of transcription (97 kDa, Protein from Rattus norvegicus)
- B27a (76 kDa, Protein from Murid betaherpesvirus 8)
- Ternary complex of rcmv-e e27 with human ddb1 (deltabpb) and rat stat2 (100 kDa, Complex from Murid betaherpesvirus 8)
- E27 (Complex from Murid betaherpesvirus 8)
- Zinc ion (65 Da, Ligand)
- Dna damage-binding protein 1 (93 kDa, Protein from Homo sapiens)
Cryo-EM structure of RCMV-E E27 bound to human DDB1 (deltaBPB) and full-length rat STAT2
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7znn
Q-score: 0.139
Le-Trilling VTK, Banchenko S, Paydar D, Leipe PM, Binting L, Lauer S, Graziadei A, Klingen R, Gotthold C, Burger J, Bracht T, Sitek B, Jan Lebbink R, Malyshkina A, Mielke T, Rappsilber J, Spahn CM, Voigt S, Trilling M, Schwefel D
EMBO J (2023) 42 pp. e112351-e112351 [ Pubmed: 36762436 DOI: doi:10.15252/embj.2022112351 ]
- Zinc ion (65 Da, Ligand)
- E27 (Complex from Murid betaherpesvirus 8)
- B27a (76 kDa, Protein from Murid betaherpesvirus 8)
- Signal transducer and activator of transcription (97 kDa, Protein from Rattus norvegicus)
- Dna damage-binding protein 1 (93 kDa, Protein from Homo sapiens)
- Signal transducer and activator of transcription (Complex from Rattus norvegicus)
- Dna damage-binding protein 1 (Complex from Homo sapiens)
- Ternary complex of rcmv-e e27 with human ddb1 (deltabpb) and rat stat2 (100 kDa, Complex from Murid betaherpesvirus 8)
Rabbit 80S ribosome as it decodes the Sec-UGA codon
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7zjw
Q-score: 0.514
Hilal T, Killam BY, Grozdanovic M, Dobosz-Bartoszek M, Loerke J, Burger J, Mielke T, Copeland PR, Simonovic M, Spahn CMT
Science (2022) 376 pp. 1338-1343 [ Pubmed: 35709277 DOI: doi:10.1126/science.abg3875 ]
- 60s ribosomal protein ul22 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el13 (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el20 (20 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l9 (21 kDa, Protein from Oryctolagus cuniculus)
- Eefsec and secis binding protein 2 (Complex from Homo sapiens)
- 40s ribosomal protein us11 (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us9 (19 kDa, Protein from Oryctolagus cuniculus)
- Gpx4 secis element (Complex from Rattus norvegicus)
- 60s ribosomal protein l6 (33 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el28 (15 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l15 (24 kDa, Protein from Oryctolagus cuniculus)
- 18s rrna (603 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein el32 (15 kDa, Protein from Oryctolagus cuniculus)
- 5s rrna (38 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein ul1 (24 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s28 (7 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3a (30 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l14 (23 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein es19 (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s24 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s25 (13 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el30 (12 kDa, Protein from Oryctolagus cuniculus)
- Crpv ires (345 kDa, RNA from Cricket paralysis virus)
- 40s ribosomal protein s6 (28 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el22 (14 kDa, Protein from Oryctolagus cuniculus)
- Sodium ion (22 Da, Ligand)
- 60s ribosomal protein el8 (30 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us15 (17 kDa, Protein from Oryctolagus cuniculus)
- Secis binding protein 2 (95 kDa, Protein from Homo sapiens)
- 60s ribosomal protein el42 (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s5 (22 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l37 (11 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein p0 (34 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein ul23 (17 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s27 (9 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l29 (26 kDa, Protein from Oryctolagus cuniculus)
- Gpx4 secis element (275 kDa, RNA from Rattus norvegicus)
- 40s ribosomal protein s11 (18 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el43 (10 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l40 (14 kDa, Protein from Oryctolagus cuniculus)
- Human ser-trna-sec (28 kDa, RNA from Homo sapiens)
- 40s ribosomal protein sa (32 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein ul13 (23 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el38 (8 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein ul29 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3 (31 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein ul2 (28 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l34 (13 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein es4 (29 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l10 (24 kDa, Protein from Oryctolagus cuniculus)
- 5.8s rrna (50 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s7 (47 kDa, Protein from Oryctolagus cuniculus)
- Water (18 Da, Ligand)
- 60s ribosomal protein l36 (12 kDa, Protein from Oryctolagus cuniculus)
- 80s-selenosome (3 MDa, Complex)
- 60s ribosomal protein el39 (6 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein ul30 (29 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l26 (17 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l19 (23 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27 (15 kDa, Protein from Oryctolagus cuniculus)
- Eefsec (67 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s23 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s27a (18 kDa, Protein from Oryctolagus cuniculus)
- Serine (105 Da, Ligand)
- 60s ribosomal protein el31 (14 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el33 (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein es26 (13 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal_l18_c domain-containing protein (34 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el18 (21 kDa, Protein from Oryctolagus cuniculus)
- Crpv ires (Complex from Cricket paralysis virus)
- 28s rrna (1 MDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l23 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s12 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s15a (14 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el21 (18 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s8 (24 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein es17 (15 kDa, Protein from Oryctolagus cuniculus)
- Potassium ion (39 Da, Ligand)
- 80s ribosome (Complex from Oryctolagus cuniculus)
- 40s ribosomal protein es21 (9 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s9 (22 kDa, Protein from Oryctolagus cuniculus)
- Ser-trna-sec (Complex from Homo sapiens)
- 40s ribosomal protein s30 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein es10 (18 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us14 (6 kDa, Protein from Oryctolagus cuniculus)
- Rack1 (35 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l4 (46 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us10 (13 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el24 (17 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27a (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us19 (17 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s18 (17 kDa, Protein from Oryctolagus cuniculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l11 (20 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us5 (31 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l3 (46 kDa, Protein from Oryctolagus cuniculus)
Rabbit 80S ribosome programmed with SECIS and SBP2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7zjx
Q-score: 0.428
Hilal T, Killam BY, Grozdanovic M, Dobosz-Bartoszek M, Loerke J, Burger J, Mielke T, Copeland PR, Simonovic M, Spahn CMT
Science (2022) 376 pp. 1338-1343 [ Pubmed: 35709277 DOI: doi:10.1126/science.abg3875 ]
- 60s ribosomal protein ul22 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el13 (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l32 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein es26 (13 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el20 (20 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s13 (17 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l9 (21 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us11 (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s17 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us9 (19 kDa, Protein from Oryctolagus cuniculus)
- Gpx4 secis element (Complex from Rattus norvegicus)
- 60s ribosomal protein l6 (33 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s20 (13 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el28 (15 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l26 (17 kDa, Protein from Oryctolagus cuniculus)
- 18s rrna (603 kDa, RNA from Oryctolagus cuniculus)
- Ribosomal protein l15 (24 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s28 (7 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3a (30 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l14 (23 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein es19 (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s24 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s25 (13 kDa, Protein from Oryctolagus cuniculus)
- Selenocysteine insertion sequence-binding protein 2 (95 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s6 (28 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el22 (14 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el8 (30 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s29 (6 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l31 (14 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l38 (8 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l35a (12 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el42 (12 kDa, Protein from Oryctolagus cuniculus)
- S10_plectin domain-containing protein (18 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s5 (22 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l30 (12 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s27 (9 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l29 (26 kDa, Protein from Oryctolagus cuniculus)
- Gpx4 secis element (275 kDa, RNA from Rattus norvegicus)
- 40s ribosomal protein s11 (18 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el43 (10 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l40 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein sa (32 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein ul13 (23 kDa, Protein from Oryctolagus cuniculus)
- 80s-sbp2-secis (3 MDa, Complex)
- Ubiquitin carboxyl extension protein 80 (18 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3 (31 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein ul2 (28 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l34 (13 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l24 (17 kDa, Protein from Oryctolagus cuniculus)
- Crpv ires (52 kDa, RNA from Cricket paralysis virus)
- 5s rrna (38 kDa, RNA from Oryctolagus cuniculus)
- 5.8s rrna (50 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s7 (47 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l36 (12 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el39 (6 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein ul30 (29 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l19 (23 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s4 (29 kDa, Protein from Oryctolagus cuniculus)
- Selenocysteine insertion sequence-binding protein 2 (Complex from Homo sapiens)
- 40s ribosomal protein s23 (15 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l37 (11 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein ul3 (46 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal_l18_c domain-containing protein (34 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein el18 (21 kDa, Protein from Oryctolagus cuniculus)
- Crpv ires (Complex from Cricket paralysis virus)
- 28s rrna (1 MDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l23 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s12 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s15a (14 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l35 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s8 (24 kDa, Protein from Oryctolagus cuniculus)
- Guanine nucleotide-binding protein subunit beta-2-like 1 (35 kDa, Protein from Oryctolagus cuniculus)
- 80s ribosome (Complex from Oryctolagus cuniculus)
- 40s ribosomal protein es21 (9 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s9 (22 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s30 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s15 (17 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal_l23en domain-containing protein (17 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l4 (46 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27a (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s18 (17 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l11 (20 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l21 (18 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us5 (31 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l10 (24 kDa, Protein from Oryctolagus cuniculus)
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7piu
Q-score: 0.6
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ DOI: doi:10.1038/s41422-021-00569-8 Pubmed: 34561620 ]
- Camelid antibody fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Ternary complex of setmelanotide-activated melanocortin 4 receptor with heterotrimeric gs, further stabilized by addition of nanobody 35 (144 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Water (18 Da, Ligand)
- Setmelanotide (other names rm-493; bim-22493; irc-022493; imcivree) (1 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 7piv
Q-score: 0.539
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ Pubmed: 34561620 DOI: doi:10.1038/s41422-021-00569-8 ]
- Calcium ion (40 Da, Ligand)
- Ndp-alpha-msh (other names afamelanotide; scenesse) (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Camelid antibody vhh fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
- Ternary complex of the ndp-alpha-msh activated melanocortin 4 receptor (mc4r) gs protein complex stabilized by nanobody 35 (144 MDa, Complex from Homo sapiens)