Wiechert F, Unbehaun A, Sprink T, Seibel H, Burger J, Loerke J, Mielke T, Diebolder CA, Schacherl M, Spahn CMT
Nucleic Acids Res (2025) 53 [ Pubmed: 39658079 DOI: doi:10.1093/nar/gkae1191 ]
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- Spermine (202 Da, Ligand)
- Large ribosomal subunit protein el40 (14 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul2 (28 kDa, Protein from Homo sapiens)
- Spermidine (145 Da, Ligand)
- Large ribosomal subunit protein ul30 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 1,4-diaminobutane (88 Da, Ligand)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- Human mature large ribosomal subunit (Complex from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
Lassa GPC (strain Josiah) bound to rabbit polyclonal base-targeting antibody Base-1
Brouwer PJM, Perrett HR, Nijhuis H, Lee W-H, Burger J, Lloyd NM, Himansu S, Beaumont T, Carfi A, Bukreyev A, Sanders RW, van Gils MJ, Ward AB
To Be Published
- Polyclonal antibody base-1 heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus)
- Glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal antibody base-1 heavy chain, polyclonal antibody base-1 light chain (Complex from Oryctolagus cuniculus)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Polyclonal antibody base-1 light chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal base-targeting antibody base-1 (Complex)
Escherichia coli paused disome complex (Non-rotated disome interface class 1)
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8rcl
Q-score: 0.307
Flugel T, Schacherl M, Unbehaun A, Schroeer B, Dabrowski M, Burger J, Mielke T, Sprink T, Diebolder CA, Guillen Schlippe YV, Spahn CMT
Nat Commun (2024) 15 pp. 1756-1756 [ DOI: doi:10.1038/s41467-024-46092-3 Pubmed: 38409277 ]
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- Trna-phe (p-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
- Trna-arg (e-site) (24 kDa, RNA from Escherichia coli)
- Nascent chain (1 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Messenger rna (20 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
- Trna-ala (a-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Alanine (89 Da, Ligand)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Trna-val (a-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- Escherichia coli pause disome complex (2 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Trna-trp (p-site) (24 kDa, RNA from Escherichia coli)
- Spermidine (145 Da, Ligand)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 1,4-diaminobutane (88 Da, Ligand)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
Escherichia coli paused disome complex (queueing 70S non-rotated closed PRE state)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8peg
Q-score: 0.363
Flugel T, Schacherl M, Unbehaun A, Schroeer B, Dabrowski M, Burger J, Mielke T, Sprink T, Diebolder CA, Guillen Schlippe YV, Spahn CMT
Nat Commun (2024) 15 pp. 1756-1756 [ DOI: doi:10.1038/s41467-024-46092-3 Pubmed: 38409277 ]
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul10 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- Nascent chain (1 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- Trna-ser (e-site) (25 kDa, RNA from Escherichia coli)
- Trna-val (a-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Trna-phe (p-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Mrna (9 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- Escherichia coli paused disome complex (2 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Escherichia coli paused disome complex (leading 70S non-rotated closed PRE state)
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8pkl
Q-score: 0.414
Flugel T, Schacherl M, Unbehaun A, Schroeer B, Dabrowski M, Burger J, Mielke T, Sprink T, Diebolder CA, Guillen Schlippe YV, Spahn CMT
Nat Commun (2024) 15 pp. 1756-1756 [ DOI: doi:10.1038/s41467-024-46092-3 Pubmed: 38409277 ]
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- Trna-ala (a-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (947 kDa, RNA from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Alanine (89 Da, Ligand)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- Spermidine (145 Da, Ligand)
- Large ribosomal subunit protein ul10 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- Nascent peptide (3 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Mrna (7 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (497 kDa, RNA from Escherichia coli)
- 1,4-diaminobutane (88 Da, Ligand)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- Escherichia coli paused disome complex (2 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- Trna-arg (e-site) (24 kDa, RNA from Escherichia coli)
- Trna-trp (p-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Escherichia coli paused disome complex (non-rotated disome interface)
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8r3v
Q-score: 0.351
Flugel T, Schacherl M, Unbehaun A, Schroeer B, Dabrowski M, Burger J, Mielke T, Sprink T, Diebolder CA, Guillen Schlippe YV, Spahn CMT
Nat Commun (2024) 15 pp. 1756-1756 [ DOI: doi:10.1038/s41467-024-46092-3 Pubmed: 38409277 ]
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- Trna-ala (a-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Nascent chain (1 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
- Alanine (89 Da, Ligand)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- Spermidine (145 Da, Ligand)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- Messenger rna (20 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Escherichia coli paused disome complex (2 MDa, Complex from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- Trna-val (a-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Trna-phe (p-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- 1,4-diaminobutane (88 Da, Ligand)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- Trna-arg (e-site) (24 kDa, RNA from Escherichia coli)
- Trna-trp (p-site) (24 kDa, RNA from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Escherichia coli paused disome complex (Non-rotated disome interface class 2)
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 8rcm
Q-score: 0.294
Flugel T, Schacherl M, Unbehaun A, Schroeer B, Dabrowski M, Burger J, Mielke T, Sprink T, Diebolder CA, Guillen Schlippe YV, Spahn CMT
Nat Commun (2024) 15 pp. 1756-1756 [ DOI: doi:10.1038/s41467-024-46092-3 Pubmed: 38409277 ]
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- Trna-phe (p-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
- Trna-arg (e-site) (24 kDa, RNA from Escherichia coli)
- Nascent chain (1 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Messenger rna (20 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
- Trna-ala (a-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Alanine (89 Da, Ligand)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Trna-val (a-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Escherichia coli paused disome complex (2 MDa, Complex from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- Trna-trp (p-site) (24 kDa, RNA from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 1,4-diaminobutane (88 Da, Ligand)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
Escherichia coli paused disome complex (Rotated disome interface class 1)
Resolution: 4.46 Å
EM Method: Single-particle
Fitted PDBs: 8rcs
Q-score: 0.218
Flugel T, Schacherl M, Unbehaun A, Schroeer B, Dabrowski M, Burger J, Mielke T, Sprink T, Diebolder CA, Guillen Schlippe YV, Spahn CMT
Nat Commun (2024) 15 pp. 1756-1756 [ DOI: doi:10.1038/s41467-024-46092-3 Pubmed: 38409277 ]
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Nascent chain (1 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- Trna-phe (p/e-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- Spermidine (145 Da, Ligand)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Trna-val (a/p-site) (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- Trna-trp (p/e-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- Trna-ala (a/p-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Messenger rna (19 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- 1,4-diaminobutane (88 Da, Ligand)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- Escherichia coli paused disome complex (2 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Escherichia coli paused disome complex (Rotated disome interface class 2)
Resolution: 5.32 Å
EM Method: Single-particle
Fitted PDBs: 8rct
Q-score: 0.181
Flugel T, Schacherl M, Unbehaun A, Schroeer B, Dabrowski M, Burger J, Mielke T, Sprink T, Diebolder CA, Guillen Schlippe YV, Spahn CMT
Nat Commun (2024) 15 pp. 1756-1756 [ DOI: doi:10.1038/s41467-024-46092-3 Pubmed: 38409277 ]
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Nascent chain (1 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- Trna-phe (p/e-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- Spermidine (145 Da, Ligand)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Trna-val (a/p-site) (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- Trna-trp (p/e-site) (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- Trna-ala (a/p-site) (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Messenger rna (19 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- 1,4-diaminobutane (88 Da, Ligand)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- Escherichia coli paused disome complex (2 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Cryo-EM structure of the plant 80S ribosome
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8b2l
Q-score: 0.639
Smirnova J, Loerke J, Kleinau G, Schmidt A, Burger J, Meyer EH, Mielke T, Scheerer P, Bock R, Spahn CMT, Zoschke R
Nat Plants (2023) 9 pp. 987-1000 [ DOI: doi:10.1038/s41477-023-01407-y Pubmed: 37156858 ]
- 40s ribosomal protein s26 (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s25 (11 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l41 (3 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l7a (29 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l34 (13 kDa, Protein from Nicotiana tabacum)
- El31 (60s ribosomal protein l31) (13 kDa, Protein from Nicotiana tabacum)
- El28 (60s ribosomal protein l28) (15 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l39-3 (6 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s24 (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s5-like (23 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s9-2-like (23 kDa, Protein from Nicotiana tabacum)
- Ribosomal protein l15 (24 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s27 (9 kDa, Protein from Nicotiana tabacum)
- 50s ribosomal protein l22, chloroplastic (20 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s3a (29 kDa, Protein from Nicotiana tabacum)
- 5s rrna (38 kDa, RNA from Nicotiana tabacum)
- 40s ribosomal protein s16 (16 kDa, Protein from Nicotiana tabacum)
- 18s rrna (583 kDa, RNA from Nicotiana tabacum)
- Ul16 (60s ribosomal protein l10) (25 kDa, Protein from Nicotiana tabacum)
- El6 (60s ribosomal protein l6) (25 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s3-2-like (27 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l13 (23 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s15-like (17 kDa, Protein from Nicotiana tabacum)
- Ul5 (60s ribosomal protein l11) (20 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l38 (8 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l14-1 (15 kDa, Protein from Nicotiana tabacum)
- Ul15 (60s ribosomal protein l27a) (16 kDa, Protein from Nicotiana tabacum)
- Spermine (202 Da, Ligand)
- 40s ribosomal protein s4 (29 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s6 (28 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l44 (12 kDa, Protein from Nicotiana tabacum)
- Zinc ion (65 Da, Ligand)
- Spermidine (145 Da, Ligand)
- 40s ribosomal protein s8 (25 kDa, Protein from Nicotiana tabacum)
- Nascent chain (2 kDa, Protein from Nicotiana tabacum)
- Ribosomal protein l19 (25 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l32-1 (15 kDa, Protein from Nicotiana tabacum)
- El36 (60s ribosomal protein l36) (12 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l24-like (18 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l23a (17 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s20-2 (13 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein sa (35 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s10-1-like (23 kDa, Protein from Nicotiana tabacum)
- Ul2 (60s ribosomal protein l8) (28 kDa, Protein from Nicotiana tabacum)
- Ubiquitin-40s ribosomal protein s27a-like (17 kDa, Protein from Nicotiana tabacum)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l23 (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s23 (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s28 (7 kDa, Protein from Nicotiana tabacum)
- 25s rrna (1 MDa, RNA from Nicotiana tabacum)
- 40s ribosomal protein s21 (9 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l37a (10 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l29 (7 kDa, Protein from Nicotiana tabacum)
- Water (18 Da, Ligand)
- 60s ribosomal protein l5-like (34 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l30-like (12 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l18-2-like (20 kDa, Protein from Nicotiana tabacum)
- Ul24 (60s ribosomal protein l26) (16 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s30 (6 kDa, Protein from Nicotiana tabacum)
- Guanine nucleotide-binding protein subunit beta-like protein (36 kDa, Protein from Nicotiana tabacum)
- Ul4 (60s ribosomal protein l4) (44 kDa, Protein from Nicotiana tabacum)
- Ul13 (60s ribosomal protein l13a) (23 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l7-4-like (28 kDa, Protein from Nicotiana tabacum)
- Magnesium ion (24 Da, Ligand)
- El33 (60s ribosomal protein l35a) (12 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l18a (21 kDa, Protein from Nicotiana tabacum)
- 30s ribosomal protein s15, chloroplastic (17 kDa, Protein from Nicotiana tabacum)
- Potassium ion (39 Da, Ligand)
- Trna (24 kDa, RNA from Nicotiana tabacum)
- Ul3 (60s ribosomal protein l3) (44 kDa, Protein from Nicotiana tabacum)
- 40s subunit of the cytosolic 80s ribosome from tobacco (Complex from Nicotiana tabacum)
- 40s ribosomal protein s11-like (17 kDa, Protein from Nicotiana tabacum)
- Mrna (3 kDa, RNA from Nicotiana tabacum)
- 60s ribosomal protein l22-2-like (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s18 (17 kDa, Protein from Nicotiana tabacum)
- Ul6 (60s ribosomal protein l9) (22 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s19-3 (16 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l21-1-like (18 kDa, Protein from Nicotiana tabacum)
- Ribosomal protein l37 (10 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s14-2 (16 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l35-like (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s29 (6 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s2-3-like (30 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s12 (15 kDa, Protein from Nicotiana tabacum)
- 5.8s rrna (52 kDa, RNA from Nicotiana tabacum)
- 40s ribosomal protein s15a-1 (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s17-like (16 kDa, Protein from Nicotiana tabacum)
- El27 (60s ribosomal protein l27) (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s7 (22 kDa, Protein from Nicotiana tabacum)