Transcription termination intermediate complex 3 delta NusG
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7adc
Q-score: 0.259
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ Pubmed: 33243850 DOI: doi:10.1126/science.abd1673 ]
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Transcription termination intermediate complex 3 delta nusg (800 kDa, Complex)
- Transcription termination intermediate complex 3 delta nusg (Complex from Escherichia coli)
- Ntdna, tdna and rut rna (Complex from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Water (18 Da, Ligand)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli)
- Ntdna (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (15 kDa, DNA from synthetic construct)
- Rut rna (31 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
Transcription termination intermediate complex IIIa
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7add
Q-score: 0.217
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ Pubmed: 33243850 DOI: doi:10.1126/science.abd1673 ]
- Transcription termination intermediate complex iiia (800 kDa, Complex)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Ntdna, tdna and rut rna (Complex from synthetic construct)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Transcription termination intermediate complex iiia (Complex from Escherichia coli)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli)
- Ntdna (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (15 kDa, DNA from synthetic construct)
- Rut rna (31 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
Transcription termination complex IVa
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7ade
Q-score: 0.235
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ Pubmed: 33243850 DOI: doi:10.1126/science.abd1673 ]
- Ntdna (15 kDa, DNA from synthetic construct)
- Beryllium trifluoride ion (66 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Ntdna, tdna and rut rna (Complex from synthetic construct)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription termination complex iva (Complex from Escherichia coli)
- Transcription termination complex iva (800 kDa, Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (15 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Rut rna (31 kDa, RNA from synthetic construct)
Transcription termination intermediate complex 1 delta NusG
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7adb
Q-score: 0.186
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ Pubmed: 33243850 DOI: doi:10.1126/science.abd1673 ]
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Rna, dna (Complex from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Transcription termination intermediate complex 1 delta nusg (Complex from Escherichia coli)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli)
- Ntdna (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (15 kDa, DNA from synthetic construct)
- Rut rna (31 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Transcription termination intermediate complex 1 delta nusg (800 kDa, Complex)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
map of the RNA polymerase lambda-based antitermination complex solved by cryo-EM
Resolution: 9.8 Å
EM Method: Single-particle
Fitted PDBs: 5ms0
Q-score: 0.069
Said N, Krupp F, Anedchenko E, Santos KF, Dybkov O, Huang YH, Lee CT, Loll B, Behrmann E, Burger J, Mielke T, Loerke J, Urlaub H, Spahn CMT, Weber G, Wahl MC
Nat Microbiol (2017) 2 pp. 17062-17062 [ DOI: doi:10.1038/nmicrobiol.2017.62 Pubmed: 28452979 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Rna transcription bubble (4 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Antitermination protein n (9 kDa, Protein from Escherichia phage lambda)
- Dnaii (11 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription antitermination protein nusb (15 kDa, Protein from Escherichia coli K-12)
- Nascent rna (9 kDa, RNA from Escherichia coli)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli K-12)
- Lambdan-dependent antitermination complex (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dnai (8 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)