High-resolution Cryo-EM of Fab-labeled human parechovirus 3
Domanska A, Flatt JW, Jukonen JJJ, Geraets JA, Butcher SJ
J. Virol. (2019) 93 [ Pubmed: 30463974 DOI: 10.1128/JVI.01597-18 ]
Image sets:
Flatt JW, Domanska A, Seppälä AL, Butcher SJ
Commun Biol (2021) 4 [ DOI: 10.1038/s42003-021-01779-x Pubmed: 33637854 ]
Image sets:
- Un-aligned multiframe micrographs of CVB4 control
- Aligned non dose weighted micrographs
- Aligned multi-frame micrographs dose weighted
- Unaligned multiframe micrographs of CVB4 in complex with CP48
Coxsackievirus B4 in complex with capsid binder compound 48
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6zck
Q-score: 0.624
Flatt JW, Domanska A, Seppala AL, Butcher SJ
Commun Biol (2021) 4 pp. 250-250 [ Pubmed: 33637854 DOI: doi:10.1038/s42003-021-01779-x ]
- 4-[(6-propoxynaphthalen-2-yl)sulfonylamino]benzoic acid (385 Da, Ligand)
- Capsid protein vp4 (7 kDa, Protein from Coxsackievirus B4 (strain E2))
- Capsid protein vp2 (27 kDa, Protein from Coxsackievirus B4 (strain E2))
- Capsid protein vp3 (26 kDa, Protein from Coxsackievirus B4 (strain E2))
- Coxsackievirus b4 (strain e2) (Virus from Coxsackievirus B4 (strain E2))
- Capsid protein vp1 (30 kDa, Protein from Coxsackievirus B4 (strain E2))
Coxsackievirus B3 in complex with capsid binder compound 17
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6zcl
Q-score: 0.62
Flatt JW, Domanska A, Seppala AL, Butcher SJ
Commun Biol (2021) 4 pp. 250-250 [ Pubmed: 33637854 DOI: doi:10.1038/s42003-021-01779-x ]
- Capsid protein vp1 (30 kDa, Protein from Coxsackievirus B3 (strain Nancy))
- Capsid protein vp3 (26 kDa, Protein from Coxsackievirus B3 (strain Nancy))
- Myristic acid (228 Da, Ligand)
- 4-[[4-[1,3-bis(oxidanylidene)isoindol-2-yl]phenyl]sulfonylamino]benzoic acid (422 Da, Ligand)
- Capsid protein vp2 (27 kDa, Protein from Coxsackievirus B3 (strain Nancy))
- Coxsackievirus b3 (strain nancy) (Virus from Coxsackievirus B3 (strain Nancy))
- Capsid protein vp4 (7 kDa, Protein from Coxsackievirus B3 (strain Nancy))
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6zms
Q-score: 0.451
Flatt JW, Domanska A, Seppala AL, Butcher SJ
Commun Biol (2021) 4 pp. 250-250 [ Pubmed: 33637854 DOI: doi:10.1038/s42003-021-01779-x ]
- Capsid protein vp4 (7 kDa, Protein from Coxsackievirus B4 (strain E2))
- Capsid protein vp2 (27 kDa, Protein from Coxsackievirus B4 (strain E2))
- Capsid protein vp3 (26 kDa, Protein from Coxsackievirus B4 (strain E2))
- Coxsackievirus b4 (strain e2) (Virus from Coxsackievirus B4 (strain E2))
- Capsid protein vp1 (30 kDa, Protein from Coxsackievirus B4 (strain E2))
Identification of a druggable VP1-VP3 interprotomer pocket in the capsid of enteroviruses
Abdelnabi R, Geraets JA, Ma Y, Mirabelli C, Flatt JW, Domanska A, Delang L, Jochmans D, Kumar TA, Jayaprakash V, Sinha BN, Leyssen P, Butcher SJ, Neyts J
PLoS Biol. (2019) 17 [ DOI: 10.1371/journal.pbio.3000281 Pubmed: 31185007 ]
Image sets:
Identification of a druggable VP1-VP3 interprotomer pocket in the capsid of enteroviruses
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6gzv
Q-score: 0.454
Abdelnabi R, Geraets JA, Ma Y, Mirabelli C, Flatt JW, Domanska A, Delang L, Jochmans D, Kumar TA, Jayaprakash V, Sinha BN, Leyssen P, Butcher SJ, Neyts J
PLoS Biol (2019) 17 pp. e3000281-e3000281 [ DOI: doi:10.1371/journal.pbio.3000281 Pubmed: 31185007 ]
- Capsid protein vp3 (26 kDa, Protein from Coxsackievirus B3 (strain Nancy))
- Capsid protein vp4 (7 kDa, Protein from Coxsackievirus B3 (strain Nancy))
- Capsid protein vp1 (31 kDa, Protein from Coxsackievirus B3 (strain Nancy))
- Capsid protein vp2 (28 kDa, Protein from Coxsackievirus B3 (strain Nancy))
- 4-[[4-[1,3-bis(oxidanylidene)isoindol-2-yl]phenyl]sulfonylamino]benzoic acid (422 Da, Ligand)
- Coxsackievirus b3 (strain nancy) (Virus from Coxsackievirus B3 (strain Nancy))
High-resolution Cryo-EM of Fab-labeled human parechovirus 3
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6gv4
Q-score: 0.434
Domanska A, Flatt JW, Jukonen JJJ, Geraets JA, Butcher SJ
J Virol (2019) 93 [ Pubmed: 30463974 DOI: doi:10.1128/JVI.01597-18 ]
- Vp1 (25 kDa, Protein from Human parechovirus 3)
- Human parechovirus type 3 in complex with fabs from at12-015 (7 MDa, Complex)
- Vp3 (28 kDa, Protein from Human parechovirus 3)
- Rna (5'-r(*up*gp*gp*up*ap*up*up*u)-3') (2 kDa, RNA from Human parechovirus 3)
- At12-015 antibody variable heavy (13 kDa, Protein from Homo sapiens)
- Fabs from at12-015 (Complex from Homo sapiens)
- Human parechovirus 3 (Virus from Human parechovirus 3)
- Vp0 (31 kDa, Protein from Human parechovirus 3)
- At12-015 antibody variable light (12 kDa, Protein from Homo sapiens)