Resolution: 42.2 Å
EM Method: Single-particle
Fitted PDBs: 6zig
Q-score: 0.028
Spinelli S, Tremblay D, Moineau S, Cambillau C, Goulet A
Viruses (2020) 12 [ Pubmed: 32796652 DOI: doi:10.3390/v12080878 ]
- Distal tail protein (34 kDa, Protein from Lactococcus phage p2)
- Receptor binding protein (28 kDa, Protein from Lactococcus phage p2)
- Lactococcus virus p2 (Virus from Lactococcus virus P2)
- Baseplate protein gp16 (42 kDa, Protein from Lactococcus phage p2)
Negative staining 3D reconstruction of p2 virion baseplate in activated conformation
Resolution: 28.7 Å
EM Method: Single-particle
Fitted PDBs: 6zih
Q-score: 0.033
Spinelli S, Tremblay D, Moineau S, Cambillau C, Goulet A
Viruses (2020) 12 [ Pubmed: 32796652 DOI: doi:10.3390/v12080878 ]
- Distal tail protein (34 kDa, Protein from Lactococcus phage p2)
- Receptor binding protein (28 kDa, Protein from Lactococcus phage p2)
- Lactococcus virus p2 (Virus from Lactococcus virus P2)
- Baseplate protein gp16 (42 kDa, Protein from Lactococcus phage p2)
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rj9
Q-score: 0.542
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Ntpam (Complex from Streptococcus virus 2972)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 monomeric assembly. (Complex)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Acriia6 (Complex from Streptococcus phage D1811)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
- Sgrna (Complex from Streptococcus thermophilus)
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rja
Q-score: 0.508
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 dimeric assembly. (Complex)
- Tdna20 (Complex from Streptococcus virus 2972)
- Acriia6 (Complex from Streptococcus phage D1811)
- Rna (78-mer) (Complex from Streptococcus thermophilus)
- Rna (78-mer) (37 kDa, RNA from Streptococcus thermophilus)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
Cryo-EM structure of St1Cas9-sgRNA-AcrIIA6-tDNA59-ntPAM complex.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rjg
Q-score: 0.522
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Ntpam (Complex from Streptococcus virus 2972)
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Ntpam (7 kDa, DNA from Streptococcus virus 2972)
- Cas 9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Cas 9 (Complex from Streptococcus thermophilus DGCC 7710)
- Tdna59 (18 kDa, DNA from Streptococcus virus 2972)
- Tdna59 (Complex from Streptococcus virus 2972)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Acriia6 (Complex from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-acriia6-tdna59-ntpam complex. (Complex)
- Sgrna (Complex from Streptococcus thermophilus)
Electron microscopy map of the T6SS TssK component
Zoued A, Durand E, Bebeacua C, Brunet YR, Douzi B, Cambillau C, Cascales E, Journet L
J Biol Chem (2013) 288 pp. 27031-27041 [ Pubmed: 23921384 DOI: doi:10.1074/jbc.M113.499772 ]
- Type vi secretion system protein tssk (160 kDa, Protein from Escherichia coli)
- Purified tssk sample (160 kDa, Sample)