Cryo-EM structure of the DHA bound FFA1-Gi complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9k1c
Q-score: 0.462
Ke Y, Huang Y, Yi C, Ma L, Chu X, Wu B, Zhao Q, Han S
Cell Rep (2024) 43 pp. 115024-115024 [ DOI: doi:10.1016/j.celrep.2024.115024 Pubmed: 39616615 ]
- Docosa-4,7,10,13,16,19-hexaenoic acid (328 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Dha bound ffa1-gi complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Free fatty acid receptor 1 (37 kDa, Protein from Homo sapiens)
Cryo-EM structure of the butyrate bound FFA2-Gi complex
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9k1d
Q-score: 0.478
Ke Y, Huang Y, Yi C, Ma L, Chu X, Wu B, Zhao Q, Han S
Cell Rep (2024) 43 pp. 115024-115024 [ DOI: doi:10.1016/j.celrep.2024.115024 Pubmed: 39616615 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Free fatty acid receptor 2 (42 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Butanoic acid (88 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cryo-em structure of the butyrate bound ffa2-gi complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the glucagon receptor bound to beta-arrestin 1 in ligand-free state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8jru
Q-score: 0.438
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ Pubmed: 37558880 DOI: doi:10.1038/s41586-023-06420-x ]
- The glucagon receptor bound to beta-arrestin 1 (Complex from Homo sapiens)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Bos taurus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
Cryo-EM structure of the glucagon receptor bound to glucagon and beta-arrestin 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jrv
Q-score: 0.431
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ Pubmed: 37558880 DOI: doi:10.1038/s41586-023-06420-x ]
- The glucagon receptor bound to glucagon and beta-arrestin 1 (Complex from Homo sapiens)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Homo sapiens)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
Cryo-EM structure of the adhesion GPCR ADGRD1 in complex with miniGs
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7wu2
Q-score: 0.53
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature (2022) 604 pp. 779-785 [ DOI: doi:10.1038/s41586-022-04580-w Pubmed: 35418679 ]
- Nanobody nb35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor d1 (38 kDa, Protein from Homo sapiens)
- Nanobody nb35 (Complex from Lama glama)
- The adhesion gpcr adgrd1 in complex with minigs (Complex from Homo sapiens)
Cryo-EM structure of the adhesion GPCR ADGRF1 in complex with miniGs
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7wu3
Q-score: 0.501
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature (2022) 604 pp. 779-785 [ DOI: doi:10.1038/s41586-022-04580-w Pubmed: 35418679 ]
- Nanobody nb35 (17 kDa, Protein from Lama glama)
- Nanobody nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (74 kDa, Protein from Homo sapiens)
- [(2~{r})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate (496 Da, Ligand)
- The adhesion gpcr adgrf1 in complex with minigs (Complex from Homo sapiens)
Cryo-EM structure of the adhesion GPCR ADGRF1 in complex with miniGi
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7wu4
Q-score: 0.428
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature (2022) 604 pp. 779-785 [ DOI: doi:10.1038/s41586-022-04580-w Pubmed: 35418679 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- The adhesion gpcr adgrf1 in complex with minigi (Complex from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (74 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- [(2~{r})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate (496 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the adhesion GPCR ADGRF1(H565A/T567A) in complex with miniGi
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7wu5
Q-score: 0.492
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature (2022) 604 pp. 779-785 [ DOI: doi:10.1038/s41586-022-04580-w Pubmed: 35418679 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- The adhesion gpcr adgrf1(h565a/t567a) in complex with minigi (Complex from Homo sapiens)
- [(2~{r})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate (496 Da, Ligand)
- Adhesion g-protein coupled receptor f1 (74 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the human formyl peptide receptor 1 in complex with fMLF and Gi1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7wvu
Q-score: 0.434
Zhu Y, Lin X, Zong X, Han S, Wang M, Su Y, Ma L, Chu X, Yi C, Zhao Q, Wu B
Nat Commun (2022) 13 pp. 1775-1775 [ DOI: doi:10.1038/s41467-022-29361-x Pubmed: 35365641 ]
- Formyl peptide receptor 1 in complex with fmlf and gi1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Fme-leu-phe (437 Da, Protein from Homo sapiens)
- Fmet-leu-phe receptor (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human formyl peptide receptor 2 in complex with fMLFII and Gi2
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7wvv
Q-score: 0.498
Zhu Y, Lin X, Zong X, Han S, Wang M, Su Y, Ma L, Chu X, Yi C, Zhao Q, Wu B
Nat Commun (2022) 13 pp. 1775-1775 [ DOI: doi:10.1038/s41467-022-29361-x Pubmed: 35365641 ]
- Fme-leu-phe-ile-ile (663 Da, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,n-formyl peptide receptor 2 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-2 (40 kDa, Protein from Homo sapiens)
- Formyl peptide receptor 2 in complex with fmlfii and gi2 (Complex from Homo sapiens)