Structures of positive allosteric modulator-bound and unbound active human calcium-sensing receptor
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
Proc Natl Acad Sci U S A (2021) 118 [ DOI: 10.1073/pnas.2115849118 Pubmed: 34916296 ]
Image sets:
Structure of negative allosteric modulator-bound inactive human calcium-sensing receptor
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
Proc Natl Acad Sci U S A (2021) 118 [ DOI: 10.1073/pnas.2115849118 Pubmed: 34916296 ]
Image sets:
Structure of positive allosteric modulator-bound active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sil
Q-score: 0.562
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of positive allosteric modulator-free active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sim
Q-score: 0.555
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of negative allosteric modulator-bound inactive human calcium-sensing receptor
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 7sin
Q-score: 0.19
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol. Cell (2020) [ Pubmed: 32386575 DOI: 10.1016/j.molcel.2020.04.014 ]
Image sets:
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Wild-type
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol. Cell (2020) [ Pubmed: 32386575 DOI: 10.1016/j.molcel.2020.04.014 ]
Image sets:
Single-Particle Cryo-EM Structure of Arabinofuranosyltransferase AftD from Mycobacteria
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6w98
Q-score: 0.535
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]
- Water (18 Da, Ligand)
- Acyl carrier protein (Cellular component from Escherichia coli (strain K12))
- Acyl carrier protein (8 kDa, Protein from Escherichia coli (strain K12))
- F5/8 type c domain-containing protein (149 kDa, Protein from Mycobacteroides abscessus subsp. abscessus)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- F5/8 type c domain-containing protein (Complex from Mycobacteroides abscessus subsp. abscessus)
- Calcium ion (40 Da, Ligand)
- Wild-type mycobacterial arabinofuranosyltransferase aftd complexed with acyl carrier protein (50 kDa, Cellular component)
- 4'-phosphopantetheine (358 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6wbx
Q-score: 0.518
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6wby
Q-score: 0.533
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]