Cryo-EM structure of FLVCR2 in the inward-facing state with choline bound
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8vzn
Q-score: 0.583
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ Pubmed: 38693257 DOI: doi:10.1038/s41586-024-07326-y ]
- Flvcr2 in the inward-facing state complexed with choline and fab flv9 (110 kDa, Complex from Mus musculus)
- Fab flv9 light chain (23 kDa, Protein from synthetic construct)
- Choline ion (104 Da, Ligand)
- Fab flv9 heavy chain (25 kDa, Protein from synthetic construct)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)
Cryo-EM structure of FLVCR2 in the outward-facing state with choline bound
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8vzo
Q-score: 0.637
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ Pubmed: 38693257 DOI: doi:10.1038/s41586-024-07326-y ]
- Fab flv23 heavy chain (25 kDa, Protein from synthetic construct)
- Flvcr2 in the outward-facing state complexed with choline and fab flv23 (110 kDa, Complex from Mus musculus)
- Fab flv23 light chain (23 kDa, Protein from synthetic construct)
- Choline ion (104 Da, Ligand)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)
Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8tj3
Q-score: 0.25
Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F
Nat Commun (2023) 14 pp. 5151-5151 [ Pubmed: 37620344 DOI: doi:10.1038/s41467-023-40483-8 ]
- Peptidoglycan d,d-transpeptidase mrda (70 kDa, Protein from Escherichia coli)
- Roda-pbp2 (111 kDa, Complex from Escherichia coli)
- Peptidoglycan glycosyltransferase mrdb (40 kDa, Protein from Escherichia coli)
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its ligand bound state
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7tpg
Q-score: 0.497
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Geranyl diphosphate (314 Da, Ligand)
- Ligand bound cmwaal with fab fragment bound (Complex)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its apo state
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7tpj
Q-score: 0.431
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Apo cmwaal with fab fragment bound (Complex)
Structure of positive allosteric modulator-bound active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sil
Q-score: 0.562
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of positive allosteric modulator-free active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sim
Q-score: 0.555
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of negative allosteric modulator-bound inactive human calcium-sensing receptor
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 7sin
Q-score: 0.19
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
cAMP-free rabbit HCN4 stabilized in LMNG-CHS detergent mixture
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7np3
Q-score: 0.419
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929-2943.e6 [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]
cAMP-bound rabbit HCN4 stabilized in LMNG-CHS detergent mixture
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7np4
Q-score: 0.451
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929-2943.e6 [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]