Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9asb
Q-score: 0.524
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ DOI: doi:10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
- Phosphate ion (94 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Chimeric mini guanine nucleotide-binding protein g(i)(s)(q) subunit alpha (7 kDa, Protein from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Chimeric mini guanine nucleotide-binding protein g(i)(s)(q) subunit alpha (28 kDa, Protein from Homo sapiens)
- Human casr in complex with chimeric gq (minigisq) protein (276 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (102 kDa, Protein from Homo sapiens)
- (19r,22s,25r)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9z)-octadec-9-enoate (749 Da, Ligand)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9avg
Q-score: 0.509
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ DOI: doi:10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
- Phosphate ion (94 Da, Ligand)
- Chimeric mini guanine nucleotide-binding protein g(i)(s) subunit alpha (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Human casr in complex with chimeric gs (minigis) protein (276 kDa, Complex from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (102 kDa, Protein from Homo sapiens)
- (19r,22s,25r)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9z)-octadec-9-enoate (749 Da, Ligand)
Structure of human calcium-sensing receptor in complex with Gi3 protein in nanodiscs
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 9avl
Q-score: 0.532
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ DOI: doi:10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
- Phosphate ion (94 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-3 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Human casr in complex with gi3 protein (288 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-2 (38 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (102 kDa, Protein from Homo sapiens)
- (19r,22s,25r)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9z)-octadec-9-enoate (749 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9axf
Q-score: 0.56
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ DOI: doi:10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
- Phosphate ion (94 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Human casr in complex with chimeric gq (minigisq) protein (319 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,adenylate cyclase-stimulating g alpha protein (28 kDa, Protein from Homo sapiens)
- Extracellular calcium-sensing receptor (102 kDa, Protein from Homo sapiens)
- Single-chain antibody fragment scfv16 (31 kDa, Protein from Mus musculus)
- Cyclomethyltryptophan (216 Da, Ligand)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Nanobody nb-35 (17 kDa, Protein from Lama glama)
Structure of human calcium-sensing receptor in complex with Gi1 (miniGi1) protein in detergent
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9ayf
Q-score: 0.529
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ DOI: doi:10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
- Phosphate ion (94 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Human casr in complex with minigi1 protein in detergent (301 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (25 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Single-chain antibody fragment scfv16 (31 kDa, Protein from Mus musculus)
- Cyclomethyltryptophan (216 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (102 kDa, Protein from Homo sapiens)
Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8tj3
Q-score: 0.25
Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F
Nat Commun (2023) 14 pp. 5151-5151 [ Pubmed: 37620344 DOI: doi:10.1038/s41467-023-40483-8 ]
- Peptidoglycan d,d-transpeptidase mrda (70 kDa, Protein from Escherichia coli)
- Roda-pbp2 (111 kDa, Complex from Escherichia coli)
- Peptidoglycan glycosyltransferase mrdb (40 kDa, Protein from Escherichia coli)
Structure of positive allosteric modulator-bound active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sil
Q-score: 0.562
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of positive allosteric modulator-free active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sim
Q-score: 0.555
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of negative allosteric modulator-bound inactive human calcium-sensing receptor
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 7sin
Q-score: 0.19
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
cAMP-free rabbit HCN4 stabilized in LMNG-CHS detergent mixture
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7np3
Q-score: 0.419
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929-2943.e6 [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]