Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F
Nat Commun (2023) 14 [ Pubmed: 37620344 DOI: 10.1038/s41467-023-40483-8 ]
Image sets:
Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8tj3
Q-score: 0.25
Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F
Nat Commun (2023) 14 pp. 5151-5151 [ Pubmed: 37620344 DOI: doi:10.1038/s41467-023-40483-8 ]
- Peptidoglycan d,d-transpeptidase mrda (70 kDa, Protein from Escherichia coli)
- Roda-pbp2 (111 kDa, Complex from Escherichia coli)
- Peptidoglycan glycosyltransferase mrdb (40 kDa, Protein from Escherichia coli)
Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F
Nat Commun (2023) 14 pp. 5151-5151 [ Pubmed: 37620344 DOI: doi:10.1038/s41467-023-40483-8 ]
- Roda-pbp2 (111 kDa, Complex from Escherichia coli)
Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F
Nat Commun (2023) 14 pp. 5151-5151 [ Pubmed: 37620344 DOI: doi:10.1038/s41467-023-40483-8 ]
- Roda-pbp2 (111 kDa, Complex from Escherichia coli)
CryoEM Structure of the, UND-PP bound, WaaL O-Antigen Ligase
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: 10.1038/s41586-022-04555-x Pubmed: 35388216 ]
Image sets:
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its ligand bound state
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7tpg
Q-score: 0.497
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Geranyl diphosphate (314 Da, Ligand)
- Ligand bound cmwaal with fab fragment bound (Complex)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its apo state
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7tpj
Q-score: 0.431
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Apo cmwaal with fab fragment bound (Complex)