In situ cryo-electron tomogram of a multi-lamellar vesicle in a NPC2-/- HeLa cell. #1
Kraus F, He Y, Swarup S, Overmyer KA, Jiang Y, Brenner J, Capitanio C, Bieber A, Jen A, Nightingale NM, Anderson BJ, Lee C, Paulo JA, Smith IR, Plitzko JM, Gygi SP, Schulman BA, Wilfling F, Coon JJ, Harper JW
Sci Adv (2025) 11 pp. eadu5787-eadu5787 [ DOI: doi:10.1126/sciadv.adu5787 Pubmed: 39841834 ]
In situ cryo-electron tomogram of a multi-lamellar vesicle in a NPC2-/- HeLa cell. #2
Kraus F, He Y, Swarup S, Overmyer KA, Jiang Y, Brenner J, Capitanio C, Bieber A, Jen A, Nightingale NM, Anderson BJ, Lee C, Paulo JA, Smith IR, Plitzko JM, Gygi SP, Schulman BA, Wilfling F, Coon JJ, Harper JW
Sci Adv (2025) 11 pp. eadu5787-eadu5787 [ DOI: doi:10.1126/sciadv.adu5787 Pubmed: 39841834 ]
Porcine epidemic diarrhea virus complete core polymerase complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8urb
Q-score: 0.49
Anderson TK, Hoferle PJ, Lee KW, Coon JJ, Kirchdoerfer RN
bioRxiv (2023) [ DOI: doi:10.1101/2023.03.15.532841 Pubmed: 36993498 ]
- Nsp7 (9 kDa, Protein from Porcine epidemic diarrhea virus)
- Zinc ion (65 Da, Ligand)
- Nsp12 (108 kDa, Protein from Porcine epidemic diarrhea virus)
- Core polymerase complex of the porcine epidemic diarrhea virus composed of nsp12, nsp7, nsp8, and a short rna substrate (185 kDa, Complex from Porcine epidemic diarrhea virus)
- Rna (55-mer) (17 kDa, RNA from synthetic construct)
- Nsp8 (21 kDa, Protein from Porcine epidemic diarrhea virus)
- Rna (33-mer) (10 kDa, RNA from synthetic construct)
Porcine epidemic diarrhea virus core polymerase complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8g6r
Q-score: 0.435
Anderson TK, Hoferle PJ, Lee KW, Coon JJ, Kirchdoerfer RN
bioRxiv (2023) [ DOI: doi:10.1101/2023.03.15.532841 Pubmed: 36993498 ]
- Zinc ion (65 Da, Ligand)
- Nsp12 (104 kDa, Protein from Porcine epidemic diarrhea virus)
- Nsp7 (6 kDa, Protein from Porcine epidemic diarrhea virus)
- Rna (5'-r(p*gp*gp*up*up*gp*up*gp*ap*up*up*up*up*ap*ap*up*ap*gp*cp*up*u)-3') (6 kDa, RNA from synthetic construct)
- Core replication complex of porcine epidemic diarrhea virus composed of nsp12, nsp7, nsp8, and a short rna substrate (185 kDa, Complex from Porcine epidemic diarrhea virus)
- Nsp8 (12 kDa, Protein from Porcine epidemic diarrhea virus)
- Rna (5'-r(p*ap*ap*gp*ap*ap*gp*cp*up*ap*up*up*ap*ap*ap*ap*up*cp*ap*cp*a)-3') (6 kDa, RNA from synthetic construct)
3D Reconstruction of PPG Matrix-Landed 20S Proteasome Core Particle Protein Complexes
Salome AZ, Lee KW, Grant T, Westphall MS, Coon JJ
Anal Chem (2022) 94 pp. 17616-17624 [ DOI: doi:10.1021/acs.analchem.2c04263 Pubmed: 36475605 ]
3D Reconstruction of PPG Matrix-Landed Beta-Galactosidase Protein Complexes
Salome AZ, Lee KW, Grant T, Westphall MS, Coon JJ
Anal Chem (2022) 94 pp. 17616-17624 [ DOI: doi:10.1021/acs.analchem.2c04263 Pubmed: 36475605 ]
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ssp
Q-score: 0.481
Manicki M, Aydin H, Abriata LA, Overmyer KA, Guerra RM, Coon JJ, Dal Peraro M, Frost A, Pagliarini DJ
Mol Cell (2022) 82 pp. 4307-4323.e10 [ Pubmed: 36306796 DOI: doi:10.1016/j.molcel.2022.10.003 ]
- Ubiquinone biosynthesis protein coq9, mitochondrial (35 kDa, Protein from Homo sapiens)
- 5-demethoxyubiquinone hydroxylase, mitochondrial (24 kDa, Protein from Homo sapiens)
- Human coq7:coq9 complex (Complex from Homo sapiens)
Structure of the NADH-bound human COQ7:COQ9 complex by single-particle electron cryo-microscopy
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7sss
Q-score: 0.599
Manicki M, Aydin H, Abriata LA, Overmyer KA, Guerra RM, Coon JJ, Dal Peraro M, Frost A, Pagliarini DJ
Mol Cell (2022) 82 pp. 4307-4323.e10 [ Pubmed: 36306796 DOI: doi:10.1016/j.molcel.2022.10.003 ]
- 5-demethoxyubiquinone hydroxylase, mitochondrial (24 kDa, Protein from Homo sapiens)
- Human coq7:coq9 complex (Complex from Homo sapiens)
- 2-[(2e,6e,10e,14e,18e,22e,26e)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaenyl]phenol (639 Da, Ligand)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Ubiquinone biosynthesis protein coq9, mitochondrial (35 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
3D Structure Determination of GroEL Protein Complexes using Matrix-Landing Mass Spectrometry
Westphall MS, Lee KW, Salome AZ, Lodge JM, Grant T, Coon JJ
Nat Commun (2022) 13 pp. 2276-2276 [ DOI: doi:10.1038/s41467-022-29964-4 Pubmed: 35478194 ]
- Groel (802 kDa, Complex from Escherichia)