Single particle cryo-EM dataset of Sars-CoV2 Rna dependent RNA polymerase
Jochheim FA, Tegunov D, Hillen HS, Schmitzová J, Kokic G, Dienemann C, Cramer P
Commun Biol (2021) 4 [ Pubmed: 34429502 DOI: 10.1038/s42003-021-02529-9 ]
Image sets:
Dimeric form of SARS-CoV-2 RNA-dependent RNA polymerase
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 7oyg
Q-score: 0.151
Jochheim FA, Tegunov D, Hillen HS, Schmitzova J, Kokic G, Dienemann C, Cramer P
Communications Biology (2021) 4 pp. 999- [ DOI: doi:10.1038/s42003-021-02529-9 ]
- Sars-cov-2 nsp7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*cp*up*ap*cp*gp*cp*ap*gp*up*g)-3') (4 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*up*gp*cp*ap*cp*up*gp*cp*gp*up*ap*g)-3') (18 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Dimeric form of sars-cov-2 rna-dependent rna polymerase complex (nsp12, nsp7, nsp8) (Complex from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 nsp8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Sars-cov-2 rna-dependent rna polymerase (nsp12) (107 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 RdRp with Molnupiravir/ NHC in the template strand base-paired with A
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ozu
Q-score: 0.522
Kabinger F, Stiller C, Schmitzova J, Dienemann C, Kokic G, Hillen HS, Hobartner C, Cramer P
Nat Struct Mol Biol (2021) 28 pp. 740-746 [ Pubmed: 34381216 DOI: doi:10.1038/s41594-021-00651-0 ]
- Template rna (10 kDa, RNA from synthetic construct)
- Rdrp (183 MDa, Complex)
- Replicase polyprotein 1ab (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Product rna (10 kDa, RNA from synthetic construct)
- Non-structural protein 8 (24 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 RdRp with Molnupiravir/ NHC in the template strand base-paired with G
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ozv
Q-score: 0.525
Kabinger F, Stiller C, Schmitzova J, Dienemann C, Kokic G, Hillen HS, Hobartner C, Cramer P
Nat Struct Mol Biol (2021) 28 pp. 740-746 [ Pubmed: 34381216 DOI: doi:10.1038/s41594-021-00651-0 ]
- Template rna (10 kDa, RNA from synthetic construct)
- Rdrp (183 MDa, Complex)
- Product rna (10 kDa, RNA from synthetic construct)
- Replicase polyprotein 1ab (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (24 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Structure of human mitochondrial RNase P in complex with mitochondrial pre-tRNA-Tyr
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7onu
Q-score: 0.585
Bhatta A, Dienemann C, Cramer P, Hillen HS
Nat Struct Mol Biol (2021) 28 pp. 713-723 [ Pubmed: 34489609 DOI: doi:10.1038/s41594-021-00637-y ]
- Magnesium ion (24 Da, Ligand)
- Mitochondrial precursor trna-tyr (41 kDa, RNA from Homo sapiens)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Mitochondrial ribonuclease p catalytic subunit (62 kDa, Protein from Homo sapiens)
- Human mitochondrial rnase p complex with precursor trna-tyr substrate (254 kDa, Complex)
- Zinc ion (65 Da, Ligand)
- Trna methyltransferase 10 homolog c (42 kDa, Protein from Homo sapiens)
- 3-hydroxyacyl-coa dehydrogenase type-2 (26 kDa, Protein from Homo sapiens)
- Human mitochondrial rnase p complex with precursor mitochondrial trna-tyr (Complex from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7b3b
Q-score: 0.501
Kokic G, Hillen HS, Tegunov D, Dienemann C, Seitz F, Schmitzova J, Farnung L, Siewert A, Hobartner C, Cramer P
Nat Commun (2021) 12 pp. 279-279 [ Pubmed: 33436624 DOI: doi:10.1038/s41467-020-20542-0 ]
- Sars-cov-2 nsp7 and nsp8 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Dna/rna (5'-r(p*cp*up*ap*cp*gp*cp*g)-d(p*(rmp))-r(p*up*g)-3') (4 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 rna-dependent rna polymerase complex (nsp12, nsp7, nsp8) with elongation scaffold containing remdesivir at position -3. (Complex)
- Dna and rna (Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase nsp12 (107 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*up*gp*cp*ap*up*cp*gp*cp*gp*up*ap*g)-3') (18 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 rna-dependent rna polymerase (nsp12) (Complex from Severe acute respiratory syndrome coronavirus 2)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7b3c
Q-score: 0.455
Kokic G, Hillen HS, Tegunov D, Dienemann C, Seitz F, Schmitzova J, Farnung L, Siewert A, Hobartner C, Cramer P
Nat Commun (2021) 12 pp. 279-279 [ Pubmed: 33436624 DOI: doi:10.1038/s41467-020-20542-0 ]
- Dna/rna (5'-r(p*cp*up*ap*cp*gp*cp*a)-d(p*(rmp))-r(p*gp*up*g)-3') (4 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 nsp7 and 8 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Dna and rna (Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase nsp12 (107 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*up*cp*ap*cp*up*up*gp*cp*gp*up*ap*g)-3') (18 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 rna-dependent rna polymerase complex (nsp12, nsp7, nsp8) with elongation scaffold containing remdesivir at position -4. (Complex)
- Sars-cov-2 rna-dependent rna polymerase (nsp12) (Complex from Severe acute respiratory syndrome coronavirus 2)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7b3d
Q-score: 0.558
Kokic G, Hillen HS, Tegunov D, Dienemann C, Seitz F, Schmitzova J, Farnung L, Siewert A, Hobartner C, Cramer P
Nat Commun (2021) 12 pp. 279-279 [ Pubmed: 33436624 DOI: doi:10.1038/s41467-020-20542-0 ]
- Sars-cov-2 rna-dependent rna polymerase nsp12 (107 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 nsp7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*cp*up*ap*cp*gp*cp*ap*gp*up*g)-3') (4 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 rna-dependent rna polymerase nsp12 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*up*gp*cp*ap*cp*up*gp*cp*gp*up*ap*g)-3') (18 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 nsp8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 nsp7 and 8 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Dna and rna (Complex from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Sars-cov-2 rna-dependent rna polymerase complex (nsp12, nsp7, nsp8) with elongation scaffold containing amp at position -4. (Complex)
Structure of replicating SARS-CoV-2 polymerase
Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P
Nature (2020) [ Pubmed: 32438371 DOI: 10.1038/s41586-020-2368-8 ]
Image sets:
- Reextraced particles at 1.3 A/px (for Map2 and Map3)
- Warp picked and extracted particles
- Refined particles (Map 1)
- Refined particles (Map 2)
- Refined particles (Map 3)
- Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 2)
- Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 1)
Structure of replicating SARS-CoV-2 polymerase
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6yyt
Q-score: 0.504
Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P
Nature (2020) 584 pp. 154-156 [ DOI: doi:10.1038/s41586-020-2368-8 Pubmed: 32438371 ]
- Rna-dependent rna polymerase complex consisting of nsp12, nsp8, nsp7 and duplex rna. (186 kDa, Complex)
- Duplex rna (Complex from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Rna product (5 kDa, RNA from synthetic construct)
- Nsp8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Nsp12 (107 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Nsp7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Nsp8, nsp7 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Nsp12 (Complex from Severe acute respiratory syndrome coronavirus 2)