CryoEM Structure of a FtsH Helical Assembly in the Presence of ATP
Resolution: 2.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 8vw9
Q-score: 0.659
Li Y, Zhu J, Zhang Z, Wei J, Wang F, Meisl G, Knowles TPJ, Egelman EH, Tezcan FA
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01811-1 Pubmed: 39806067 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cryo-em structure of ftsh helical assembly (Complex from Thermotoga maritima)
- Atp-dependent zinc metalloprotease ftsh (51 kDa, Protein from Thermotoga maritima)
CryoEM Structure of a FtsH Helical Assembly in the Presence of ATP
Resolution: 2.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 8vwa
Q-score: 0.665
Li Y, Zhu J, Zhang Z, Wei J, Wang F, Meisl G, Knowles TPJ, Egelman EH, Tezcan FA
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01811-1 Pubmed: 39806067 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Atp-dependent zinc metalloprotease ftsh (51 kDa, Protein from Thermotoga maritima)
- Cryoem structure of ftsh helical assembly (Complex from Thermotoga maritima)
CryoEM Structure of a FtsH Helical Assembly in the Aged State
Resolution: 2.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 8vwb
Q-score: 0.649
Li Y, Zhu J, Zhang Z, Wei J, Wang F, Meisl G, Knowles TPJ, Egelman EH, Tezcan FA
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01811-1 Pubmed: 39806067 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cryoem structure of a ftsh helical assembly in the aged state (Complex from Thermotoga maritima)
- Zinc ion (65 Da, Ligand)
- Atp-dependent zinc metalloprotease ftsh (51 kDa, Protein from Thermotoga maritima)
CryoEM Structure of a FtsH Helical Assembly in the Aged State
Resolution: 2.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 8vwc
Q-score: 0.664
Li Y, Zhu J, Zhang Z, Wei J, Wang F, Meisl G, Knowles TPJ, Egelman EH, Tezcan FA
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01811-1 Pubmed: 39806067 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Atp-dependent zinc metalloprotease ftsh (51 kDa, Protein from Thermotoga maritima)
- Cryo-em structure of ftsh helical assembly in the aged state (Complex from Thermotoga maritima)
Ex vivo cannulae fiber structure of Pyrodictium abyssi
Resolution: 2.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 9h8b
Q-score: 0.647
Sleutel M, Sonani RR, Miller JG, Wang F, Socorro AG, Chen Y, Martin R, Demeler B, Rudolph MJ, Alva V, Remaut H, Egelman EH, Conticello VP
bioRxiv (2024) [ Pubmed: 39803462 DOI: doi:10.1101/2024.12.30.630787 ]
- Canx (18 kDa, Protein from Pyrodictium abyssi DSM 6158)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Ex vivo canx fibers from pyrodictium abyssi (Complex from Pyrodictium abyssi DSM 6158)
Cryo-EM of tail-tip of bacteriophage Chi
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8vjh
Q-score: 0.341
Sonani RR, Esteves NC, Scharf BE, Egelman EH
Structure (2024) 32 pp. 856-865.e3 [ DOI: doi:10.1016/j.str.2024.03.011 Pubmed: 38614087 ]
- Minor tail protein (143 kDa, Protein from Chivirus chi)
- Chivirus chi (Virus from Chivirus chi)
- Distal tail protein (63 kDa, Protein from Chivirus chi)
- Tail tube (40 kDa, Protein from Chivirus chi)
- Baseplate hub 1 protein, gp27 (29 kDa, Protein from Chivirus chi)
- Tape measure (153 kDa, Protein from Chivirus chi)
- Tail assembly protein 1 (8 kDa, Protein from Chivirus chi)
- Fe (iii) ion (55 Da, Ligand)
Structure of baseplate with receptor binding complex of Agrobacterium phage Milano
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8fqc
Q-score: 0.498
Sonani RR, Palmer LK, Esteves NC, Horton AA, Sebastian AL, Kelly RJ, Wang F, Kreutzberger MAB, Russell WK, Leiman PG, Scharf BE, Egelman EH
Nat Commun (2024) 15 pp. 756-756 [ DOI: doi:10.1038/s41467-024-44959-z Pubmed: 38272938 ]
- Tail-tube, gp21 (14 kDa, Protein from Agrobacterium phage Milano)
- Baseplate centerpiece, gp25 (43 kDa, Protein from Agrobacterium phage Milano)
- Tail sheath protein, gp20 (53 kDa, Protein from Agrobacterium phage Milano)
- Tail spike protein, gp124 (61 kDa, Protein from Agrobacterium phage Milano)
- Baseplate hub protein, gp26 (49 kDa, Protein from Agrobacterium phage Milano)
- Baseplate wedge 1, gp28 (19 kDa, Protein from Agrobacterium phage Milano)
- Baseplate central spike, gp27 (20 kDa, Protein from Agrobacterium phage Milano)
- Agrobacterium phage milano (Virus from Agrobacterium phage Milano)
- Baseplate wedge 3 protein, gp30 (31 kDa, Protein from Agrobacterium phage Milano)
- Baseplate wedge 2 protein, gp29 (43 kDa, Protein from Agrobacterium phage Milano)
- Short tail fibers, gp31 (31 kDa, Protein from Agrobacterium phage Milano)
- Fe (iii) ion (55 Da, Ligand)
Atomic structure of Salmonella SipA/F-actin complex by cryo-EM
Resolution: 3.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 8uee
Q-score: 0.506
Niedzialkowska E, Runyan LA, Kudryashova E, Egelman EH, Kudryashov DS
Structure (2024) 32 pp. 725-738.e8 [ DOI: doi:10.1016/j.str.2024.02.022 Pubmed: 38518780 ]
- Phosphate ion (94 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Cell invasion protein sipa (28 kDa, Protein from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Sipa (Complex)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Actin, alpha skeletal muscle (Complex)
- Salmonella sipa/f-actin complex (Complex from Oryctolagus cuniculus)
Cryo-EM of self-assembled cannula CanA
Resolution: 2.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7uii
Q-score: 0.642
Wang F, Miller JG, Egelman EH, Conticello VP
To Be Published
- Cana (Complex from Pyrodictium abyssi)
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
- Cana (19 kDa, Protein from Pyrodictium abyssi)
Cryo-EM structure of F-actin/Plastin2-ABD2 complex
Resolution: 3.9 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vec
Q-score: 0.385
Schwebach CL, Kudryashova E, Zheng W, Orchard M, Smith H, Runyan LA, Egelman EH, Kudryashov DS
Bone Res (2020) 8 pp. 21-21 [ Pubmed: 32509377 DOI: doi:10.1038/s41413-020-0095-2 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Lcp1 (47 kDa, Protein from Homo sapiens)
- Plastin2-abd2 (Complex from Homo sapiens)
- F-actin (Complex from Oryctolagus cuniculus)
- Complex of plastin2-abd2 with f-actin (Complex)