Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 8e3f
Q-score: 0.158
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
- T dna (18 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Escherichia coli rho-dependent transcription pre-termination complex containing 18 nt long rna spacer, mg-adp-bef3, and nusg, tec part (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Nt dna (18 kDa, DNA from synthetic construct)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna with 18 nt long spacer (11 kDa, RNA from synthetic construct)
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 8e3h
Q-score: 0.06
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Escherichia coli rho-dependent transcription pre-termination complex containing 18 nt long rna spacer, mg-adp-bef3, and nusg; rho hexamer part (Complex from Escherichia coli)
- Rna with 18 nt long spacer (11 kDa, RNA from synthetic construct)
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8e5k
Q-score: 0.228
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
- T dna (18 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Nt dna (18 kDa, DNA from synthetic construct)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna with 21 nt long spacer (11 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Escherichia coli rho-dependent transcription pre-termination complex containing 21 nt long rna spacer, mg-adp-bef3, and nusg, tec part (Complex from Escherichia coli)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8e5l
Q-score: 0.148
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Escherichia coli rho-dependent transcription pre-termination complex containing 21 nt long rna spacer, mg-adp-bef3, and nusg; rho hexamer part (Complex from Escherichia coli)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Rna with 21 nt long spacer (11 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8e5o
Q-score: 0.151
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
- T dna (18 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Nt dna (18 kDa, DNA from synthetic construct)
- Escherichia coli rho-dependent transcription pre-termination complex containing 24 nt long rna spacer, mg-adp-bef3, and nusg; tec part (Complex from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna with 24 nt long spacer (12 kDa, RNA from synthetic construct)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8e5p
Q-score: 0.13
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Escherichia coli rho-dependent transcription pre-termination complex containing 21 nt long rna spacer, mg-adp-bef3, and nusg; rho hexamer part (Complex from Escherichia coli)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Rna with 24 nt long spacer (12 kDa, RNA from synthetic construct)
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 8e6w
Q-score: 0.151
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ DOI: doi:10.1038/s41586-022-05658-1 Pubmed: 36697824 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Escherichia coli rho-dependent transcription pre-termination complex containing 18 nt long rna spacer, lambda-tr1 rut rna, mg-adp-bef3, and nusg; rho part (Complex from Escherichia coli)
- Lambda-tr1 rut rna (19 kDa, RNA from synthetic construct)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)