Energy barriers and driving forces of tRNA translocation through the ribosome
Resolution: 12.0 Å
EM Method: Single-particle
Fitted PDBs: 4v6z
Q-score: 0.015
Bock LV, Blau C, Schroeder GF, Davydov II, Fischer N, Stark H, Rodnina MV, Vaiana AC, Grubmueller H
Nat Struct Mol Biol (2013) 20 pp. 1390-1396 [ DOI: doi:10.1038/nsmb.2690 Pubmed: 24186064 ]
- M022 mrna (RNA from synthetic construct)
- Ribosome (2 MDa, Complex from Escherichia coli)
- Fmetval-trnaval (25 kDa, RNA from Escherichia coli)
- Substate of e. coli 70s-fmetval-trnaval-trnafmet complex in classic pre-translocation state (pre1b) (2 MDa, Sample)
- Trnafmet (25 kDa, RNA from Escherichia coli)
Energy barriers and driving forces of tRNA translocation through the ribosome
Resolution: 15.0 Å
EM Method: Single-particle
Fitted PDBs: 4v73
Q-score: 0.061
Bock LV, Blau C, Schroeder GF, Davydov II, Fischer N, Stark H, Rodnina MV, Vaiana AC, Grubmueller H
Nat Struct Mol Biol (2013) 20 pp. 1390-1396 [ DOI: doi:10.1038/nsmb.2690 Pubmed: 24186064 ]
- Substrate of e. coli 70s-fmetval-trnaval-trnafmet complex in hybrid pre-translocation state (pre5a) (2 MDa, Sample)
- M022 mrna (RNA from synthetic construct)
- Ribosome (2 MDa, Complex from Escherichia coli)
- Fmetval-trnaval (25 kDa, RNA from Escherichia coli)
- Trnafmet (25 kDa, RNA from Escherichia coli)
Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy
Resolution: 12.0 Å
EM Method: Single-particle
Fitted PDBs: 4v6y
Q-score: 0.018
Fischer N, Konevega AL, Wintermeyer W, Rodnina MV, Stark H
Nature (2010) 466 pp. 329-333 [ Pubmed: 20631791 DOI: doi:10.1038/nature09206 ]
- M022 mrna (RNA from synthetic construct)
- Fmetval-trnaval (25 kDa, RNA from Escherichia coli)
- E.coli 70s (2 MDa, Complex from Escherichia coli)
- Substate of e. coli 70s-fmetval-trnaval-trnafmet complex in classic pre-translocation state (pre1) (2 MDa, Sample)
- Trnafmet (25 kDa, RNA from Escherichia coli)
Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy
Resolution: 13.0 Å
EM Method: Single-particle
Fitted PDBs: 4v72
Q-score: 0.072
Fischer N, Konevega AL, Wintermeyer W, Rodnina MV, Stark H
Nature (2010) 466 pp. 329-333 [ Pubmed: 20631791 DOI: doi:10.1038/nature09206 ]
- M022 mrna (RNA from synthetic construct)
- Ribosome (2 MDa, Complex from Escherichia coli)
- Substate of e. coli 70s-fmetval-trnaval-trnafmet complex in hybrid pre-translocation state (pre4) (2 MDa, Sample)
- Fmetval-trnaval (25 kDa, RNA from Escherichia coli)
- Trnafmet (25 kDa, RNA from Escherichia coli)
Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy
Resolution: 12.0 Å
EM Method: Single-particle
Fitted PDBs: 4v75
Q-score: 0.072
Fischer N, Konevega AL, Wintermeyer W, Rodnina MV, Stark H
Nature (2010) 466 pp. 329-333 [ Pubmed: 20631791 DOI: doi:10.1038/nature09206 ]
- M022 mrna (RNA from synthetic construct)
- Ribosome (2 MDa, Complex from Escherichia coli)
- Substate of e. coli 70s-fmetval-trnaval-trnafmet complex in classic post-translocation state (post1) (2 MDa, Sample)
- Fmetval-trnaval (25 kDa, RNA from Escherichia coli)
- Trnafmet (25 kDa, RNA from Escherichia coli)
Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy
Resolution: 15.0 Å
EM Method: Single-particle
Fitted PDBs: 4v79
Q-score: 0.051
Fischer N, Konevega AL, Wintermeyer W, Rodnina MV, Stark H
Nature (2010) 466 pp. 329-333 [ Pubmed: 20631791 DOI: doi:10.1038/nature09206 ]
- M022 mrna (RNA from synthetic construct)
- Ribosome (2 MDa, Complex from Escherichia coli)
- Substate of e. coli 70s-fmetval-trnaval-trnafmet complex in intermediate post-translocation state (post3) (2 MDa, Sample)
- Fmetval-trnaval (25 kDa, RNA from Escherichia coli)
- Trnafmet (25 kDa, RNA from Escherichia coli)
Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy
Fischer N, Konevega AL, Wintermeyer W, Rodnina MV, Stark H
Nature (2010) 466 pp. 329-333 [ Pubmed: 20631791 DOI: doi:10.1038/nature09206 ]
- E. coli 70s-fmetval-trnaval post-translocation complex at 18 degrees c (2 MDa, Sample)
- M022 mrna (RNA from synthetic construct)
- Fmetval-trnaval (25 kDa, RNA from Escherichia coli)
- Ribosome (2 MDa, Complex from Escherichia coli)
Spontaneous reverse movement of mRNA-bound tRNA through the ribosome.
Konevega AL, Fischer N, Semenkov YP, Stark H, Wintermeyer W, Rodnina MV
Nat Struct Mol Biol (2007) 14 pp. 318-324 [ DOI: doi:10.1038/nsmb1221 Pubmed: 17369838 ]