Structure of the membrane-bound human mitoribosome
Englmeier R, Pfeffer S, Forster F
Structure (2017) 25 pp. 1574-1581.e2 [ Pubmed: 28867615 DOI: doi:10.1016/j.str.2017.07.011 ]
In situ subtomogram average of Rubisco within the Chlamydomonas pyrenoid
Freeman Rosenzweig ES, Xu B, Kuhn Cuellar L, Martinez-Sanchez A, Schaffer M, Strauss M, Cartwright HN, Ronceray P, Plitzko JM, Forster F, Wingreen NS, Engel BD, Mackinder LCM, Jonikas MC
Cell (2017) 171 pp. 148-162.e19 [ Pubmed: 28938114 DOI: doi:10.1016/j.cell.2017.08.008 ]
- In situ rubisco holoenzyme (540 kDa, Complex from Chlamydomonas reinhardtii)
- Rubisco holoenzyme (Protein)
KaiCB circadian clock backbone model based on a Cryo-EM density
Snijder J, Schuller JM, Wiegard A, Lossl P, Schmelling N, Axmann IM, Plitzko JM, Forster F, Heck AJ
Science (2017) 355 pp. 1181-1184 [ Pubmed: 28302852 DOI: doi:10.1126/science.aag3218 ]
KaiCBA circadian clock backbone model based on a Cryo-EM density
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 5n8y
Q-score: 0.286
Snijder J, Schuller JM, Wiegard A, Lossl P, Schmelling N, Axmann IM, Plitzko JM, Forster F, Heck AJ
Science (2017) 355 pp. 1181-1184 [ Pubmed: 28302852 DOI: doi:10.1126/science.aag3218 ]
- Circadian clock protein kinase kaic (58 kDa, Protein from Synechococcus elongatus)
- Circadian clock protein kaia (32 kDa, Protein from Synechococcus elongatus)
- Kaicba circadian clock complex (Complex from Synechococcus elongatus)
- Circadian clock protein kaib (11 kDa, Protein from Synechococcus elongatus)
26S proteasome in presence of ATP (s1)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5mp9, 5mpd
Q-score: 0.2815
Wehmer M, Rudack T, Beck F, Aufderheide A, Pfeifer G, Plitzko JM, Forster F, Schulten K, Baumeister W, Sakata E
PNAS (2017) 114 pp. 1305-1310 [ Pubmed: 28115689 DOI: doi:10.1073/pnas.1621129114 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-6 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease subunit rpt4 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 7 homolog (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-5 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 6b homolog (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-1 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 26s protease regulatory subunit 4 homolog (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome of saccharomyces cerevisiae in presence of atp (s1) (1 MDa, Complex from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-2 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 6a (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-7 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26S proteasome in presence of ATP (s2)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5mpa, 5mpe
Q-score: 0.2305
Wehmer M, Rudack T, Beck F, Aufderheide A, Pfeifer G, Plitzko JM, Forster F, Schulten K, Baumeister W, Sakata E
PNAS (2017) 114 pp. 1305-1310 [ Pubmed: 28115689 DOI: doi:10.1073/pnas.1621129114 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-6 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome of saccharomyces cerevisiae in presence of atp (s2) (1 MDa, Complex from Saccharomyces cerevisiae S288c)
- 26s protease subunit rpt4 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 7 homolog (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-5 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 6b homolog (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-1 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 26s protease regulatory subunit 4 homolog (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-2 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 6a (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-7 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26S proteasome in presence of AMP-PNP (s3)
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 5mpb
Q-score: 0.132
Wehmer M, Rudack T, Beck F, Aufderheide A, Pfeifer G, Plitzko JM, Forster F, Schulten K, Baumeister W, Sakata E
PNAS (2017) 114 pp. 1305-1310 [ Pubmed: 28115689 DOI: doi:10.1073/pnas.1621129114 ]
- 26s proteasome regulatory subunit rpn1 (109 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-3 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn10 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn2 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-6 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease subunit rpt4 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn3 (60 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 7 homolog (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome complex subunit sem1 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome of saccharomyces cerevisiae in presence of amp-pnp (1 MDa, Complex from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-5 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn12 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 6b homolog (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-1 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ubiquitin carboxyl-terminal hydrolase rpn11 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 4 homolog (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-2 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn7 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn8 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- 26s protease regulatory subunit 6a (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-7 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26S proteasome in presence of BeFx (s4)
Resolution: 7.7 Å
EM Method: Single-particle
Fitted PDBs: 5mpc
Q-score: 0.135
Wehmer M, Rudack T, Beck F, Aufderheide A, Pfeifer G, Plitzko JM, Forster F, Schulten K, Baumeister W, Sakata E
PNAS (2017) 114 pp. 1305-1310 [ Pubmed: 28115689 DOI: doi:10.1073/pnas.1621129114 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome regulatory subunit rpn1 (109 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome of saccharomyces cerevisiae in presence of befx (s4) (1 MDa, Complex from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn10 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn2 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-6 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease subunit rpt4 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn3 (60 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 7 homolog (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome complex subunit sem1 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-5 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn12 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 6b homolog (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin carboxyl-terminal hydrolase 6 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-1 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ubiquitin carboxyl-terminal hydrolase rpn11 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 4 homolog (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-2 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn7 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn8 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s protease regulatory subunit 6a (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-7 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pfeffer S, Dudek J, Schaffer M, Ng BG, Albert S, Plitzko JM, Baumeister W, Zimmermann R, Freeze HH, Engel BD, Forster F
Nat Commun (2017) 8 pp. 14516-14516 [ Pubmed: 28218252 DOI: doi:10.1038/ncomms14516 ]
Pfeffer S, Dudek J, Schaffer M, Ng BG, Albert S, Plitzko JM, Baumeister W, Zimmermann R, Freeze HH, Engel BD, Forster F
Nat Commun (2017) 8 pp. 14516-14516 [ Pubmed: 28218252 DOI: doi:10.1038/ncomms14516 ]