Anti-CRISPR protein AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ Pubmed: 28574055 DOI: doi:10.1038/cr.2017.79 ]
- Csy2 (Protein from Pseudomonas aeruginosa)
- Csy1 (Protein from Pseudomonas aeruginosa)
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 20nt spacer crrna (300 kDa, Complex from Pseudomonas aeruginosa)
- Acrf2 (Protein from Pseudomonas phage 73)
- Csy3 (Protein from Pseudomonas aeruginosa)
- Acrf1 (Protein from Pseudomonas phage D3)
- Csy4 (Protein from Pseudomonas aeruginosa)
- Crrna with 20nt spacer (RNA from Pseudomonas aeruginosa)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5xlo
Q-score: 0.337
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ Pubmed: 28574055 DOI: doi:10.1038/cr.2017.79 ]
- Acrf1 (Complex)
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 32nt spacer crrna backbone region (400 kDa, Complex from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crrna with 32nt spacer sequence (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (Complex)
- Crrna with 20nt spacer sequence (Complex)
- Uncharacterized protein acrf1 (8 kDa, Protein from Pseudomonas phage JBD30)
Anti-CRISPR protein AcrF1 bound to Csy surveillance complex with 32nt spacer crRNA in binding mode B
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ Pubmed: 28574055 DOI: doi:10.1038/cr.2017.79 ]
- Csy2 (Protein from Pseudomonas aeruginosa)
- Csy1 (Protein from Pseudomonas aeruginosa)
- Anti-crispr protein acrf1 bound to csy surveillance complex with 32nt spacer crrna in binding mode b (400 kDa, Complex from Pseudomonas aeruginosa)
- Crrna with 32nt spacer (RNA from Pseudomonas aeruginosa)
- Acrf1 (Protein from Pseudomonas aeruginosa)
- Csy3 (Protein from Pseudomonas aeruginosa)
- Csy4 (Protein from Pseudomonas aeruginosa)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5xlp
Q-score: 0.26
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ Pubmed: 28574055 DOI: doi:10.1038/cr.2017.79 ]
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 20nt spacer sequence backbone region (300 kDa, Complex from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crrna with 20nt spacer sequence (15 kDa, RNA from Pseudomonas aeruginosa)
- Acrf1 (Complex)
- Man crispr-associated protein csy3 (Complex)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Uncharacterized protein acrf1 (8 kDa, Protein from Pseudomonas phage JBD30)
- Rna (Complex)
Resolution: 9.0 Å
EM Method: Single-particle
Fitted PDBs: 3j3t
Q-score: 0.077
Liu J, Mei Z, Li N, Qi Y, Xu Y, Shi Y, Wang F, Lei J, Gao N
J Biol Chem (2013) 288 pp. 17597-17608 [ DOI: doi:10.1074/jbc.M113.458752 Pubmed: 23595989 ]
- Meca (Protein from Bacillus subtilis)
- Clpc (Protein from Bacillus subtilis)
- Meca-clpc(e280a) (600 kDa, Sample)
Resolution: 11.0 Å
EM Method: Single-particle
Fitted PDBs: 3j3s
Q-score: 0.104
Liu J, Mei Z, Li N, Qi Y, Xu Y, Shi Y, Wang F, Lei J, Gao N
J Biol Chem (2013) 288 pp. 17597-17608 [ DOI: doi:10.1074/jbc.M113.458752 Pubmed: 23595989 ]
- Meca (Protein from Bacillus subtilis)
- Clpc (Protein from Bacillus subtilis)
- Meca-clpc(e618a) (600 kDa, Sample)
Resolution: 10.0 Å
EM Method: Single-particle
Fitted PDBs: 3j3u
Q-score: 0.086
Liu J, Mei Z, Li N, Qi Y, Xu Y, Shi Y, Wang F, Lei J, Gao N
J Biol Chem (2013) 288 pp. 17597-17608 [ DOI: doi:10.1074/jbc.M113.458752 Pubmed: 23595989 ]
- Meca-clpc (e280a,e618a) with atp (600 kDa, Sample)
- Meca (Protein from Bacillus subtilis)
- Clpc (Protein from Bacillus subtilis)
Resolution: 9.4 Å
EM Method: Single-particle
Fitted PDBs: 3j3r
Q-score: 0.079
Liu J, Mei Z, Li N, Qi Y, Xu Y, Shi Y, Wang F, Lei J, Gao N
J Biol Chem (2013) 288 pp. 17597-17608 [ DOI: doi:10.1074/jbc.M113.458752 Pubmed: 23595989 ]
- Meca (Protein from Bacillus subtilis)
- Meca-clpc(e280a,e618a)with adp (600 kDa, Sample)
- Clpc (Protein from Bacillus subtilis)