Structure of a substrate engaged SecA-SecY protein translocation machine
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 6itc
Q-score: 0.436
Ma C, Wu X, Sun D, Park E, Catipovic MA, Rapoport TA, Gao N, Li L
Nat Commun (2019) 10 pp. 2872-2872 [ DOI: doi:10.1038/s41467-019-10918-2 Pubmed: 31253804 ]
- Green fluorescent protein (26 kDa, Protein from Aequorea victoria)
- Magnesium ion (24 Da, Ligand)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis (strain 168))
- Beryllium trifluoride ion (66 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nanobody (12 kDa, Protein from Lama glama)
- Seca-secy complex (Complex from Bacillus subtilis)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans (strain NG80-2))
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Nanobody (12 kDa, Protein from Lama glama)
- Translocating peptide (6 kDa, Protein from Escherichia coli)
- Protein translocase subunit secy (46 kDa, Protein from Geobacillus thermodenitrificans (strain NG80-2))
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 5zr1
Q-score: 0.547
Li N, Lam WH, Zhai Y, Cheng J, Cheng E, Zhao Y, Gao N, Tye BK
Nature (2018) 559 pp. 217-222 [ Pubmed: 29973722 DOI: doi:10.1038/s41586-018-0293-x ]
- 72bp-oring dna, acs305, t-rich (22 kDa, DNA from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Saccharomyces cerevisiae origin recognition complex bound to a 72-bp origin dna containing acs and b1 element (Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 72bp-oring dna, acs305, a-rich (22 kDa, DNA from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Cryo-EM structure of the Cdt1-MCM2-7 complex in AMPPNP state
Resolution: 7.1 Å
EM Method: Single-particle
Fitted PDBs: 5xf8
Q-score: 0.257
Zhai Y, Cheng E, Wu H, Li N, Yung PY, Gao N, Tye BK
Nat Struct Mol Biol (2017) 24 pp. 300-308 [ DOI: doi:10.1038/nsmb.3374 Pubmed: 28191894 ]
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cdt1-mcm2-7 (Complex from Saccharomyces cerevisiae)
