Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+1C)
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 8y9y
Q-score: 0.522
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Substrate ftsq-lacy(+1c) (7 kDa, Protein from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Seca-secy complex with the substrate ftsq-lacy(+1c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate HmBRI-3TM
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8y9z
Q-score: 0.453
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Seca-secy complex with the substrate hmbri-3tm (Complex from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Bacteriorhodopsin-i (11 kDa, Protein from Haloarcula marismortui ATCC 43049)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Nanobody (13 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8ya0
Q-score: 0.515
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit seca (87 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Seca-secy complex with the substrate ftsq-lacy(+7c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (7 kDa, Protein from Escherichia coli K-12)
- Nanobody (12 kDa, Protein from Lama glama)
- Protein translocase subunit sece (6 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Nanobody (12 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Green fluorescent protein (25 kDa, Protein from Aequorea victoria)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+20C)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 8ya2
Q-score: 0.343
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (8 kDa, Protein from Escherichia coli K-12)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Seca-secy complex with the substrate ftsq-lacy(+20c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Nanobody (12 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Green fluorescent protein (25 kDa, Protein from Aequorea victoria)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C) treated with DTT
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8ya3
Q-score: 0.48
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (8 kDa, Protein from Escherichia coli K-12)
- Seca-secy complex with the substrate ftsq-lacy(+7c) treated with dtt (Complex from Geobacillus thermodenitrificans NG80-2)
Structure of the SecA-SecY complex with the substrate HmBRI-7TM
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 8yas
Q-score: 0.29
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Seca-secy complex with the substrate hmbri-7tm (Complex from Geobacillus thermodenitrificans NG80-2)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
