Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+1C)
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 8y9y
Q-score: 0.522
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Substrate ftsq-lacy(+1c) (7 kDa, Protein from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Seca-secy complex with the substrate ftsq-lacy(+1c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate HmBRI-3TM
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8y9z
Q-score: 0.453
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Seca-secy complex with the substrate hmbri-3tm (Complex from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Bacteriorhodopsin-i (11 kDa, Protein from Haloarcula marismortui ATCC 43049)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Nanobody (13 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8ya0
Q-score: 0.515
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit seca (87 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Seca-secy complex with the substrate ftsq-lacy(+7c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (7 kDa, Protein from Escherichia coli K-12)
- Nanobody (12 kDa, Protein from Lama glama)
- Protein translocase subunit sece (6 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Nanobody (12 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Green fluorescent protein (25 kDa, Protein from Aequorea victoria)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+20C)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 8ya2
Q-score: 0.343
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (8 kDa, Protein from Escherichia coli K-12)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Seca-secy complex with the substrate ftsq-lacy(+20c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Nanobody (12 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Green fluorescent protein (25 kDa, Protein from Aequorea victoria)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C) treated with DTT
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8ya3
Q-score: 0.48
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (8 kDa, Protein from Escherichia coli K-12)
- Seca-secy complex with the substrate ftsq-lacy(+7c) treated with dtt (Complex from Geobacillus thermodenitrificans NG80-2)
Structure of the SecA-SecY complex with the substrate HmBRI-7TM
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 8yas
Q-score: 0.29
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Seca-secy complex with the substrate hmbri-7tm (Complex from Geobacillus thermodenitrificans NG80-2)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
Cryo-EM structure of the GPR101-Gs complex
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8w8q
Q-score: 0.564
Yang Z, Wang JY, Yang F, Zhu KK, Wang GP, Guan Y, Ning SL, Lu Y, Li Y, Zhang C, Zheng Y, Zhou SH, Wang XW, Wang MW, Xiao P, Yi F, Zhang C, Zhang PJ, Xu F, Liu BH, Zhang H, Yu X, Gao N, Sun JP
Nat Chem Biol (2024) 20 pp. 484-492 [ Pubmed: 37945893 DOI: doi:10.1038/s41589-023-01456-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,probable g-protein coupled receptor 101 (73 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gpr101-gs complex (Complex from Homo sapiens)
- Nb35 (13 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Cryo-EM structure of the AA-14-bound GPR101-Gs complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8w8r
Q-score: 0.558
Yang Z, Wang JY, Yang F, Zhu KK, Wang GP, Guan Y, Ning SL, Lu Y, Li Y, Zhang C, Zheng Y, Zhou SH, Wang XW, Wang MW, Xiao P, Yi F, Zhang C, Zhang PJ, Xu F, Liu BH, Zhang H, Yu X, Gao N, Sun JP
Nat Chem Biol (2024) 20 pp. 484-492 [ Pubmed: 37945893 DOI: doi:10.1038/s41589-023-01456-6 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit (42 kDa, Protein from Homo sapiens)
- Cryo-em structure of the aa14-bound gpr101-gs complex (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 101 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 1-(4-methylpyridin-2-yl)-3-[3-(trifluoromethyl)phenyl]thiourea (311 Da, Ligand)
Cryo-EM structure of the AA14-bound GPR101 complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8w8s
Q-score: 0.471
Cryo-EM structure of Mycobacterium tuberculosis 50S ribosome subunit bound with clarithromycin
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7f0d
Q-score: 0.473
Zhang W, Li Z, Sun Y, Cui P, Liang J, Xing Q, Wu J, Xu Y, Zhang W, Zhang Y, He L, Gao N
Emerg Microbes Infect (2022) 11 pp. 293-305 [ DOI: doi:10.1080/22221751.2021.2022439 Pubmed: 34935599 ]
- 50s ribosomal protein l13 (16 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l14 (13 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l34 (5 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l36 (4 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l20 (14 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l27 (8 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l21 (11 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l23 (10 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l31 (8 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l4 (23 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l18 (13 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l19 (13 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l3 (23 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- Water (18 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l29 (8 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 5s ribosomal rna (37 kDa, RNA from Mycobacterium tuberculosis H37Ra)
- Mycobacterium tuberculosis ribosome 50s subunit (Complex)
- Clarithromycin (747 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l30 (7 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal subunit (Complex from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l24 (11 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l2 (30 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l17 (19 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l6 (19 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l25 (22 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l22 (20 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l5 (21 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 23s rrna (1 MDa, RNA from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l28 (6 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l15 (15 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l33 (6 kDa, Protein from Mycobacterium tuberculosis H37Ra)
- 50s ribosomal protein l9 (16 kDa, Protein from Mycobacterium tuberculosis H37Ra)