Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9arv
Q-score: 0.311
Xiang Y, Xu J, McGovern BL, Ranzenigo A, Huang W, Sang Z, Shen J, Diaz-Tapia R, Pham ND, Teunissen AJP, Rodriguez ML, Benjamin J, Taylor DJ, van Leent MMT, White KM, Garcia-Sastre A, Zhang P, Shi Y
Cell (2024) 187 pp. 6966-6980.e23 [ Pubmed: 39447570 DOI: doi:10.1016/j.cell.2024.09.043 ]
- Immunoglobulin j chain (19 kDa, Protein from Homo sapiens)
- Isoform 1 of immunoglobulin heavy constant mu (41 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Adaptive multi-epitope targeting and avidity-enhanced (ameta) nanobody platform (Complex from Homo sapiens)
CryoEM map of anchor 222-1C06 Fab and lateral patch 2B05 Fab binding H1 HA
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 7t3d
Q-score: 0.464
Guthmiller JJ, Han J, Utset HA, Li L, Lan LY, Henry C, Stamper CT, McMahon M, O'Dell G, Fernandez-Quintero ML, Freyn AW, Amanat F, Stovicek O, Gentles L, Richey ST, de la Pena AT, Rosado V, Dugan HL, Zheng NY, Tepora ME, Bitar DJ, Changrob S, Strohmeier S, Huang M, Garcia-Sastre A, Liedl KR, Bloom JD, Nachbagauer R, Palese P, Krammer F, Coughlan L, Ward AB, Wilson PC
Nature (2022) 602 pp. 314-320 [ Pubmed: 34942633 DOI: doi:10.1038/s41586-021-04356-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hemagglutinin (Complex from Influenza A virus (A/California/04/2009(H1N1)))
- Cryoem map of anchor 222-1c06 fab and lateral patch 2b05 fab binding h1 ha (Complex from Homo sapiens)
- Hemagglutinin ha2 chain (19 kDa, Protein from Influenza A virus (A/California/04/2009(H1N1)))
- Hemagglutinin ha1 chain (36 kDa, Protein from Influenza A virus (A/California/04/2009(H1N1)))
- Monoclonal antibodies 222-1c06 and 2b05 (Complex from Homo sapiens)
- 222-1c06 mab light chain (11 kDa, Protein from Homo sapiens)
- 222-1c06 mab heavy chain (13 kDa, Protein from Homo sapiens)
- 2b05 mab light chain (11 kDa, Protein from Homo sapiens)
- 2b05 mab heavy chain (12 kDa, Protein from Homo sapiens)
SARS-CoV-2 Spike bound to Nb6 in closed conformation
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7kkk
Q-score: 0.463
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]
- Synthetic nanobody nb6 (12 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Synthetic nanobody nb6 (Complex from synthetic construct)
- Complex of spike s2p glycoprotein with synthetic nanobody nb6 (Complex)
- Spike s2p glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 Spike bound to mNb6 in closed conformation
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7kkl
Q-score: 0.514
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Synthetic nanobody mnb6 (13 kDa, Protein from synthetic construct)
- Complex of spike s2p glycoprotein with synthetic nanobody mnb6 (Complex)
- Synthetic nanobody mnb6 (Complex from synthetic construct)
- Spike s2p glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)