Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 9es7
Q-score: 0.682
Pintscher S, Pietras R, Mielecki B, Szwalec M, Wojcik-Augustyn A, Indyka P, Rawski M, Koziej L, Jaciuk M, Wazny G, Glatt S, Osyczka A
Nat Plants (2024) 10 pp. 1814-1825 [ DOI: doi:10.1038/s41477-024-01804-x Pubmed: 39362993 ]
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from Spinacia oleracea)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Spinacia oleracea)
- Heme c (618 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Undecyl-maltoside (496 Da, Ligand)
- Cytochrome f (35 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Spinacia oleracea)
- Cytochrome b6f complex incubated with decylplastoquinone (220 kDa, Complex from Spinacia oleracea)
- Water (18 Da, Ligand)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from Spinacia oleracea)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from Spinacia oleracea)
- Cytochrome b6 (24 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Spinacia oleracea)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
Resolution: 2.24 Å
EM Method: Single-particle
Fitted PDBs: 9es8
Q-score: 0.626
Pintscher S, Pietras R, Mielecki B, Szwalec M, Wojcik-Augustyn A, Indyka P, Rawski M, Koziej L, Jaciuk M, Wazny G, Glatt S, Osyczka A
Nat Plants (2024) 10 pp. 1814-1825 [ DOI: doi:10.1038/s41477-024-01804-x Pubmed: 39362993 ]
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from Spinacia oleracea)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Spinacia oleracea)
- Heme c (618 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Undecyl-maltoside (496 Da, Ligand)
- Cytochrome f (35 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Spinacia oleracea)
- Water (18 Da, Ligand)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from Spinacia oleracea)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from Spinacia oleracea)
- Cytochrome b6f complex with decylplastoquinol bound at quinone reduction site (220 kDa, Complex from Spinacia oleracea)
- Cytochrome b6 (24 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Spinacia oleracea)
- Decylplastoquinone (276 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 9es9
Q-score: 0.618
Pintscher S, Pietras R, Mielecki B, Szwalec M, Wojcik-Augustyn A, Indyka P, Rawski M, Koziej L, Jaciuk M, Wazny G, Glatt S, Osyczka A
Nat Plants (2024) 10 pp. 1814-1825 [ DOI: doi:10.1038/s41477-024-01804-x Pubmed: 39362993 ]
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from Spinacia oleracea)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Spinacia oleracea)
- Heme c (618 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Cytochrome b6f complex inhibited with dbmib (2,5-dibromo-3-isopropyl-6-methylbenzo-1,4-quinone) (220 kDa, Complex from Spinacia oleracea)
- Undecyl-maltoside (496 Da, Ligand)
- Cytochrome f (35 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Spinacia oleracea)
- Water (18 Da, Ligand)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from Spinacia oleracea)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- 2,5-dibromo-3-isopropyl-6-methylbenzo-1,4-quinone (321 Da, Ligand)
- Cytochrome b6 (24 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Spinacia oleracea)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
Human pseudouridine synthase 3 and tRNA-Gln
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8okd
Q-score: 0.455
Lin TY, Kleemann L, Jezowski J, Dobosz D, Rawski M, Indyka P, Wazny G, Mehta R, Chramiec-Glabik A, Koziej L, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S
Mol Cell (2024) 84 pp. 2472-2489.e8 [ DOI: doi:10.1016/j.molcel.2024.06.013 Pubmed: 38996458 ]
- Human trna-gln (70-mer) (24 kDa, RNA from Homo sapiens)
- Trna pseudouridine(38/39) synthase (55 kDa, Protein from Homo sapiens)
- Pseudouridine synthase 3 (pus3) (Complex from Homo sapiens)
- Homodimer of pus3 and trna-gln complex (110 kDa, Complex)
- Trna-gln (Complex from Homo sapiens)
Human pseudouridine synthase 3 (PUS3 R116A mutant) and two tRNA-Gln
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 9enb
Q-score: 0.576
Lin TY, Kleemann L, Jezowski J, Dobosz D, Rawski M, Indyka P, Wazny G, Mehta R, Chramiec-Glabik A, Koziej L, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S
Mol Cell (2024) 84 pp. 2472-2489.e8 [ DOI: doi:10.1016/j.molcel.2024.06.013 Pubmed: 38996458 ]
- Trna-gln (24 kDa, RNA from Homo sapiens)
- Trna pseudouridine(38/39) synthase (55 kDa, Protein from Homo sapiens)
- Homodimer of human pus3 and two trna-gln (160 kDa, Complex)
- Pseudouridine synthase 3 (pus3) (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Trna-gln (Complex from Homo sapiens)
Human pseudouridine synthase 3 (PUS3 R116A mutant) and one tRNA-Gln
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 9enc
Q-score: 0.464
Lin TY, Kleemann L, Jezowski J, Dobosz D, Rawski M, Indyka P, Wazny G, Mehta R, Chramiec-Glabik A, Koziej L, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S
Mol Cell (2024) 84 pp. 2472-2489.e8 [ DOI: doi:10.1016/j.molcel.2024.06.013 Pubmed: 38996458 ]
- Trna-gln (24 kDa, RNA from Homo sapiens)
- Trna pseudouridine(38/39) synthase (55 kDa, Protein from Homo sapiens)
- Pseudouridine synthase 3 (pus3) (Complex from Homo sapiens)
- Homodimer of human pus3 and one trna-gln (140 kDa, Complex)
- Trna-gln (Complex from Homo sapiens)
Human pseudouridine synthase 3 (PUS3 D118A mutant) and two tRNA-Arg
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 9ene
Q-score: 0.488
Lin TY, Kleemann L, Jezowski J, Dobosz D, Rawski M, Indyka P, Wazny G, Mehta R, Chramiec-Glabik A, Koziej L, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S
Mol Cell (2024) 84 pp. 2472-2489.e8 [ DOI: doi:10.1016/j.molcel.2024.06.013 Pubmed: 38996458 ]
- Trna-arg (24 kDa, RNA from Homo sapiens)
- Pseudouridine synthase 3 (pus3) (Complex from Homo sapiens)
- Trna pseudouridine(38/39) synthase (55 kDa, Protein from Homo sapiens)
- Trna-arg (Complex from Homo sapiens)
- Homodimer of human pus3 and two trna-arg (160 kDa, Complex)
Human pseudouridine synthase 3 (PUS3 D118A mutant) and two pre-tRNA-Arg
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 9enf
Q-score: 0.356
Lin TY, Kleemann L, Jezowski J, Dobosz D, Rawski M, Indyka P, Wazny G, Mehta R, Chramiec-Glabik A, Koziej L, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S
Mol Cell (2024) 84 pp. 2472-2489.e8 [ DOI: doi:10.1016/j.molcel.2024.06.013 Pubmed: 38996458 ]
- Homodimer of human pus3 and two pre-trna-arg (180 kDa, Complex)
- Pseudouridine synthase 3 (pus3) (Complex from Homo sapiens)
- Trna pseudouridine(38/39) synthase (55 kDa, Protein from Homo sapiens)
- Trna-arg (Complex from Homo sapiens)
- Pre-trna-arg (30 kDa, RNA from Homo sapiens)
Cryo-EM structure of human Elp123 in complex with tRNA, acetyl-CoA, 5'-deoxyadenosine and methionine
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8ptx
Q-score: 0.441
Abbassi NE, Jaciuk M, Scherf D, Bohnert P, Rau A, Hammermeister A, Rawski M, Indyka P, Wazny G, Chramiec-Glabik A, Dobosz D, Skupien-Rabian B, Jankowska U, Rappsilber J, Schaffrath R, Lin TY, Glatt S
Nat Commun (2024) 15 pp. 4094-4094 [ Pubmed: 38750017 DOI: doi:10.1038/s41467-024-48251-y ]
- Elongator complex protein 2 (92 kDa, Protein from Homo sapiens)
- 5'-deoxyadenosine (251 Da, Ligand)
- Acetyl coenzyme *a (809 Da, Ligand)
- Elongator complex protein 1 (150 kDa, Protein from Homo sapiens)
- Methionine (149 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Elongator complex protein 3 (65 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Trna gln (24 kDa, RNA from Homo sapiens)
- Human elp123 in complex with glutamine trna, acetyl-coa, 5'-deoxyadenosine and methionine (635 kDa, Complex from Homo sapiens)
Cryo-EM structure of human Elp123 in complex with 5'-deoxyadenosine and methionine
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 8pty
Q-score: 0.487
Abbassi NE, Jaciuk M, Scherf D, Bohnert P, Rau A, Hammermeister A, Rawski M, Indyka P, Wazny G, Chramiec-Glabik A, Dobosz D, Skupien-Rabian B, Jankowska U, Rappsilber J, Schaffrath R, Lin TY, Glatt S
Nat Commun (2024) 15 pp. 4094-4094 [ Pubmed: 38750017 DOI: doi:10.1038/s41467-024-48251-y ]
- Elongator complex protein 2 (92 kDa, Protein from Homo sapiens)
- 5'-deoxyadenosine (251 Da, Ligand)
- Elongator complex protein 1 (150 kDa, Protein from Homo sapiens)
- Methionine (149 Da, Ligand)
- Human elp123 in complex with 5'-deoxyadenosine and methionine (610 kDa, Complex from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Elongator complex protein 3 (65 kDa, Protein from Homo sapiens)