MicroED structure of Proteinase K from lamellae milled from multiple plasma sources
Resolution: 1.39 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyo
Q-score: 0.911
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ Pubmed: 36841804 DOI: doi:10.1038/s41467-023-36733-4 ]
- Calcium ion (40 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Nitrate ion (62 Da, Ligand)
- Water (18 Da, Ligand)
MicroED structure of Proteinase K from xenon milled lamellae
Resolution: 1.45 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyp
Q-score: 0.898
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ Pubmed: 36841804 DOI: doi:10.1038/s41467-023-36733-4 ]
- Calcium ion (40 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Nitrate ion (62 Da, Ligand)
- Water (18 Da, Ligand)
MicroED structure of Proteinase K from argon milled lamellae
Resolution: 1.4 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyq
Q-score: 0.905
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ Pubmed: 36841804 DOI: doi:10.1038/s41467-023-36733-4 ]
- Calcium ion (40 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Nitrate ion (62 Da, Ligand)
- Water (18 Da, Ligand)
MicroED structure of Proteinase K from oxygen milled lamellae
Resolution: 1.5 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyr
Q-score: 0.894
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ Pubmed: 36841804 DOI: doi:10.1038/s41467-023-36733-4 ]
- Calcium ion (40 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Nitrate ion (62 Da, Ligand)
- Water (18 Da, Ligand)
MicroED structure of Proteinase K from nitrogen milled lamellae
Resolution: 1.8 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fys
Q-score: 0.833
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ Pubmed: 36841804 DOI: doi:10.1038/s41467-023-36733-4 ]
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
MicroED structure of lysozyme from milled crystals at 1.75A
Resolution: 1.75 Å
EM Method: Electron Crystallography
Fitted PDBs: 7mrp
Q-score: 0.823
To Be Published
- Lysozyme (14 kDa, Complex from Gallus gallus)
- Sodium ion (22 Da, Ligand)
- Lysozyme c (14 kDa, Protein from Gallus gallus)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Proteinase K soaked with I3C determined by MicroED from a single milled microcrystal
Resolution: 1.78 Å
EM Method: Electron Crystallography
Fitted PDBs: 7jsy
Q-score: 0.84
Structure (2021) 29 pp. 88- [ Pubmed: 33007196 DOI: doi:10.1016/j.str.2020.09.003 ]
- 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid (558 Da, Ligand)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
1.4A low-dose structure of GSNQNNF determined from initial phases generated using radiation damage
Resolution: 1.4 Å
EM Method: Electron Crystallography
Fitted PDBs: 6vhb
Q-score: 0.832
Martynowycz MW, Hattne J, Gonen T
Structure (2020) 28 pp. 458- [ Pubmed: 32023481 DOI: doi:10.1016/j.str.2020.01.008 ]
- Zinc ion (65 Da, Ligand)
- Acetate ion (59 Da, Ligand)
- Gsnqnnf (779 Da, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Synthetic proto-filament (899 Da, Complex from synthetic construct)
MicroED structure of an oxindole product from modified trpB at 0.9A resolution
Dick M, Sarai NS, Martynowycz MW, Gonen T, Arnold FH
J Am Chem Soc (2019) 141 pp. 19817-19822 [ Pubmed: 31747522 DOI: doi:10.1021/jacs.9b09864 ]
MicroED structure of a ketone product from modified trpB at 0.9A resolution
Dick M, Sarai NS, Martynowycz MW, Gonen T, Arnold FH
J Am Chem Soc (2019) 141 pp. 19817-19822 [ Pubmed: 31747522 DOI: doi:10.1021/jacs.9b09864 ]